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Effect of Complement on HIV-2 Plasma Antiviral Activity Is Intratype Specific and Potent

Özkaya Sahin, Gülsen LU ; Holmgren, Birgitta G LU ; Sheik-Khalil, Enas LU ; da Silva, Zacarias; Nielsen, Jens; Nowroozalizadeh, Salma LU ; Månsson, Fredrik LU ; Norrgren, Hans LU ; Aaby, Peter and Fenyö, Eva Maria LU , et al. (2013) In Journal of Virology 87(1). p.273-281
Abstract
Human immunodeficiency virus type-2 (HIV-2) infected individuals develop immunodeficiency with a considerable delay and transmit the virus at a lower rate as compared to HIV-1 infected. Conceivably, comparative studies on immune responsiveness of the HIV-1 and HIV-2 infected hosts may help to explain differences in pathogenesis and transmission between the two types of infection. Previous studies have shown that the neutralizing antibody response is more potent and broader in HIV-2 than HIV-1 infection. In the present study we have further examined the function of the humoral immune response and studied the potentiating effect of complement (C') on antiviral activity of plasma from singly HIV-1 or HIV-2 infected, as well as HIV-1/HIV-2... (More)
Human immunodeficiency virus type-2 (HIV-2) infected individuals develop immunodeficiency with a considerable delay and transmit the virus at a lower rate as compared to HIV-1 infected. Conceivably, comparative studies on immune responsiveness of the HIV-1 and HIV-2 infected hosts may help to explain differences in pathogenesis and transmission between the two types of infection. Previous studies have shown that the neutralizing antibody response is more potent and broader in HIV-2 than HIV-1 infection. In the present study we have further examined the function of the humoral immune response and studied the potentiating effect of complement (C') on antiviral activity of plasma from singly HIV-1 or HIV-2 infected, as well as HIV-1/HIV-2 dually infected individuals. Neutralization and antibody-dependent complement-mediated inactivation of HIV-1 and HIV-2 isolates were tested in a plaque reduction assay using U87.CD4-CCR5 cells. Results showed that addition of C' increased intra-type antiviral activity of both HIV-1 and HIV-2 plasma, although the C' effect was more pronounced with HIV-2 than HIV-1 plasma. Using the area-under-curve (AUC)-based readout, multivariate statistical analysis confirmed that type of HIV infection was independently associated with the magnitude of the C' effect. Analysis carried out with purified IgG indicated that the C' effect was largely exerted through the classical C' pathway involving IgG in both HIV-1 and HIV-2 infections. In summary, these findings suggest that antibody binding to HIV-2 structures facilitates efficient use of C', and may thereby be one factor contributing to a strong antiviral activity present in HIV-2 infection. (Less)
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Journal of Virology
volume
87
issue
1
pages
273 - 281
publisher
American Society for Microbiology
external identifiers
  • wos:000312455500022
  • pmid:23077299
  • scopus:84871939861
ISSN
1098-5514
DOI
10.1128/JVI.01640-12
language
English
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yes
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ff4f8a23-6ca4-4f67-9971-6842888c6b56 (old id 3160590)
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http://www.ncbi.nlm.nih.gov/pubmed/23077299?dopt=Abstract
date added to LUP
2012-11-01 13:26:13
date last changed
2017-07-02 04:02:09
@article{ff4f8a23-6ca4-4f67-9971-6842888c6b56,
  abstract     = {Human immunodeficiency virus type-2 (HIV-2) infected individuals develop immunodeficiency with a considerable delay and transmit the virus at a lower rate as compared to HIV-1 infected. Conceivably, comparative studies on immune responsiveness of the HIV-1 and HIV-2 infected hosts may help to explain differences in pathogenesis and transmission between the two types of infection. Previous studies have shown that the neutralizing antibody response is more potent and broader in HIV-2 than HIV-1 infection. In the present study we have further examined the function of the humoral immune response and studied the potentiating effect of complement (C') on antiviral activity of plasma from singly HIV-1 or HIV-2 infected, as well as HIV-1/HIV-2 dually infected individuals. Neutralization and antibody-dependent complement-mediated inactivation of HIV-1 and HIV-2 isolates were tested in a plaque reduction assay using U87.CD4-CCR5 cells. Results showed that addition of C' increased intra-type antiviral activity of both HIV-1 and HIV-2 plasma, although the C' effect was more pronounced with HIV-2 than HIV-1 plasma. Using the area-under-curve (AUC)-based readout, multivariate statistical analysis confirmed that type of HIV infection was independently associated with the magnitude of the C' effect. Analysis carried out with purified IgG indicated that the C' effect was largely exerted through the classical C' pathway involving IgG in both HIV-1 and HIV-2 infections. In summary, these findings suggest that antibody binding to HIV-2 structures facilitates efficient use of C', and may thereby be one factor contributing to a strong antiviral activity present in HIV-2 infection.},
  author       = {Özkaya Sahin, Gülsen and Holmgren, Birgitta G and Sheik-Khalil, Enas and da Silva, Zacarias and Nielsen, Jens and Nowroozalizadeh, Salma and Månsson, Fredrik and Norrgren, Hans and Aaby, Peter and Fenyö, Eva Maria and Jansson, Marianne},
  issn         = {1098-5514},
  language     = {eng},
  number       = {1},
  pages        = {273--281},
  publisher    = {American Society for Microbiology},
  series       = {Journal of Virology},
  title        = {Effect of Complement on HIV-2 Plasma Antiviral Activity Is Intratype Specific and Potent},
  url          = {http://dx.doi.org/10.1128/JVI.01640-12},
  volume       = {87},
  year         = {2013},
}