Selective nucleotide-release from dense-core granules in insulin-secreting cells.
(2005) In Journal of Cell Science 118(Pt 18). p.4271-4282- Abstract
- Secretory granules of insulin-secreting cells are used to store and release peptide hormones as well as low-molecular-weight compounds such as nucleotides. Here we have compared the rate of exocytosis with the time courses of nucleotide and peptide release by a combination of capacitance measurements, electrophysiological detection of ATP release and single-granule imaging. We demonstrate that the release of nucleotides and peptides is delayed by similar to 0.1 and similar to 2 seconds with respect to membrane fusion, respectively. We further show that in up to 70% of the cases exocytosis does not result in significant release of the peptide cargo, likely because of a mechanism that leads to premature closure of the fusion pore. Release of... (More)
- Secretory granules of insulin-secreting cells are used to store and release peptide hormones as well as low-molecular-weight compounds such as nucleotides. Here we have compared the rate of exocytosis with the time courses of nucleotide and peptide release by a combination of capacitance measurements, electrophysiological detection of ATP release and single-granule imaging. We demonstrate that the release of nucleotides and peptides is delayed by similar to 0.1 and similar to 2 seconds with respect to membrane fusion, respectively. We further show that in up to 70% of the cases exocytosis does not result in significant release of the peptide cargo, likely because of a mechanism that leads to premature closure of the fusion pore. Release of nucleotides and protons occurred regardless of whether peptides were secreted or not. These observations suggest that insulin-secreting cells are able to use the same secretory vesicles to release small molecules either alone or together with the peptide hormone. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/143866
- author
- Obermüller, Stefanie LU ; Lindqvist, Anders LU ; Karanauskaite, Jovita LU ; Galvanovskis, Juris ; Rorsman, Patrik LU and Barg, Sebastian LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- insulin, hormone, fusion pore, exocytosis, endocytosis, kiss-and-run, secretion
- in
- Journal of Cell Science
- volume
- 118
- issue
- Pt 18
- pages
- 4271 - 4282
- publisher
- The Company of Biologists Ltd
- external identifiers
-
- wos:000232549000020
- pmid:16141231
- scopus:27144434554
- ISSN
- 0021-9533
- DOI
- 10.1242/jcs.02549
- language
- English
- LU publication?
- yes
- id
- ff77c11a-e395-46b9-8c7b-1bdeba8bef94 (old id 143866)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16141231&dopt=Abstract
- date added to LUP
- 2016-04-01 12:08:54
- date last changed
- 2022-04-13 06:43:40
@article{ff77c11a-e395-46b9-8c7b-1bdeba8bef94,
abstract = {{Secretory granules of insulin-secreting cells are used to store and release peptide hormones as well as low-molecular-weight compounds such as nucleotides. Here we have compared the rate of exocytosis with the time courses of nucleotide and peptide release by a combination of capacitance measurements, electrophysiological detection of ATP release and single-granule imaging. We demonstrate that the release of nucleotides and peptides is delayed by similar to 0.1 and similar to 2 seconds with respect to membrane fusion, respectively. We further show that in up to 70% of the cases exocytosis does not result in significant release of the peptide cargo, likely because of a mechanism that leads to premature closure of the fusion pore. Release of nucleotides and protons occurred regardless of whether peptides were secreted or not. These observations suggest that insulin-secreting cells are able to use the same secretory vesicles to release small molecules either alone or together with the peptide hormone.}},
author = {{Obermüller, Stefanie and Lindqvist, Anders and Karanauskaite, Jovita and Galvanovskis, Juris and Rorsman, Patrik and Barg, Sebastian}},
issn = {{0021-9533}},
keywords = {{insulin; hormone; fusion pore; exocytosis; endocytosis; kiss-and-run; secretion}},
language = {{eng}},
number = {{Pt 18}},
pages = {{4271--4282}},
publisher = {{The Company of Biologists Ltd}},
series = {{Journal of Cell Science}},
title = {{Selective nucleotide-release from dense-core granules in insulin-secreting cells.}},
url = {{http://dx.doi.org/10.1242/jcs.02549}},
doi = {{10.1242/jcs.02549}},
volume = {{118}},
year = {{2005}},
}