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14-3-3 expression in denervated hippocampus after entorhinal cortex lesion assessed by culture-derived isotope tags in quantitative proteomics

Sihlbom, Carina; Wilhelmsson, Ulrika; Li, Lizhen; Nilsson, Carol L LU and Pekny, Milos (2007) In Journal of Proteome Research 6(9). p.3491-3500
Abstract

Activation of astrocytes accompanies many brain pathologies. Reactive astrocytes have a beneficial role in acute neurotrauma but later on might inhibit regeneration. 2D-gel electrophoresis and mass spectrometry were applied to study the proteome difference in denervated hippocampus in wildtype mice and mice lacking intermediate filament proteins glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) that show attenuated reactive gliosis and enhanced posttraumatic regeneration. Proteomic data and immunohistochemical analyses showed upregulation of the adapter protein 14-3-3 four days postlesion and suggested that 14-3-3 upregulation after injury is triggered by reactive gliosis. Culture-derived isotope tags (CDIT) and mass... (More)

Activation of astrocytes accompanies many brain pathologies. Reactive astrocytes have a beneficial role in acute neurotrauma but later on might inhibit regeneration. 2D-gel electrophoresis and mass spectrometry were applied to study the proteome difference in denervated hippocampus in wildtype mice and mice lacking intermediate filament proteins glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) that show attenuated reactive gliosis and enhanced posttraumatic regeneration. Proteomic data and immunohistochemical analyses showed upregulation of the adapter protein 14-3-3 four days postlesion and suggested that 14-3-3 upregulation after injury is triggered by reactive gliosis. Culture-derived isotope tags (CDIT) and mass spectrometry demonstrated that 14-3-3 epsilon was the major isoform upregulated in denervated hippocampus and that its upregulation was attenuated in GFAP-/-Vim-/- mice and thus most likely connected to reactive gliosis.

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publishing date
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publication status
published
keywords
14-3-3 Proteins, Animals, Astrocytes, Cerebral Cortex, Electrophoresis, Gel, Two-Dimensional, Entorhinal Cortex, Glial Fibrillary Acidic Protein, Hippocampus, Mass Spectrometry, Mice, Mice, Inbred C57BL, Mice, Transgenic, Neurodegenerative Diseases, Proteomics, Vimentin, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Proteome Research
volume
6
issue
9
pages
10 pages
publisher
The American Chemical Society
external identifiers
  • scopus:34948836783
ISSN
1535-3893
DOI
10.1021/pr070108e
language
English
LU publication?
no
id
ff8e3c89-5e64-401a-98ef-f8ebaedc1697
date added to LUP
2017-05-16 10:33:35
date last changed
2017-05-21 05:01:37
@article{ff8e3c89-5e64-401a-98ef-f8ebaedc1697,
  abstract     = {<p>Activation of astrocytes accompanies many brain pathologies. Reactive astrocytes have a beneficial role in acute neurotrauma but later on might inhibit regeneration. 2D-gel electrophoresis and mass spectrometry were applied to study the proteome difference in denervated hippocampus in wildtype mice and mice lacking intermediate filament proteins glial fibrillary acidic protein (GFAP) and vimentin (GFAP-/-Vim-/-) that show attenuated reactive gliosis and enhanced posttraumatic regeneration. Proteomic data and immunohistochemical analyses showed upregulation of the adapter protein 14-3-3 four days postlesion and suggested that 14-3-3 upregulation after injury is triggered by reactive gliosis. Culture-derived isotope tags (CDIT) and mass spectrometry demonstrated that 14-3-3 epsilon was the major isoform upregulated in denervated hippocampus and that its upregulation was attenuated in GFAP-/-Vim-/- mice and thus most likely connected to reactive gliosis.</p>},
  author       = {Sihlbom, Carina and Wilhelmsson, Ulrika and Li, Lizhen and Nilsson, Carol L and Pekny, Milos},
  issn         = {1535-3893},
  keyword      = {14-3-3 Proteins,Animals,Astrocytes,Cerebral Cortex,Electrophoresis, Gel, Two-Dimensional,Entorhinal Cortex,Glial Fibrillary Acidic Protein,Hippocampus,Mass Spectrometry,Mice,Mice, Inbred C57BL,Mice, Transgenic,Neurodegenerative Diseases,Proteomics,Vimentin,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {9},
  pages        = {3491--3500},
  publisher    = {The American Chemical Society},
  series       = {Journal of Proteome Research},
  title        = {14-3-3 expression in denervated hippocampus after entorhinal cortex lesion assessed by culture-derived isotope tags in quantitative proteomics},
  url          = {http://dx.doi.org/10.1021/pr070108e},
  volume       = {6},
  year         = {2007},
}