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Influence of gut microbiota on mouse B2 B cell ontogeny and function

Hansson, Jenny LU ; Bosco, Nabil; Favre, Laurent; Raymond, Frederic; Oliveira, Manuel; Metairon, Sylviane; Mansourian, Robert; Blum, Stephanie; Kussmann, Martin and Benyacoub, Jalil (2011) In Molecular Immunology 48(9-10). p.101-1091
Abstract

A complex interplay between the microbiota and the host immune system is evidenced to shape the immune system throughout life, but little is known about the microbial effect on key players of the adaptive immune system, the B2 B cells. In the presented study, we have evaluated the effect of commensal bacteria on B cell ontogeny and function, with the focus on B2 B cells of spleen and Peyer's patches. We have compared germ-free mice to mice that are exposed to a normal complex bacterial community from the day of birth and combined classical immunological assessment with advanced genome-wide expression profiling. Despite a preservation of all B cell subsets and phenotype, our results show that microbiota strongly impact mucosal B cell... (More)

A complex interplay between the microbiota and the host immune system is evidenced to shape the immune system throughout life, but little is known about the microbial effect on key players of the adaptive immune system, the B2 B cells. In the presented study, we have evaluated the effect of commensal bacteria on B cell ontogeny and function, with the focus on B2 B cells of spleen and Peyer's patches. We have compared germ-free mice to mice that are exposed to a normal complex bacterial community from the day of birth and combined classical immunological assessment with advanced genome-wide expression profiling. Despite a preservation of all B cell subsets and phenotype, our results show that microbiota strongly impact mucosal B cell physiology and lead to higher serum Ig concentrations. We show that this microbial influence comprises downregulation of transcription factors involved in early B cell activation steps and upregulation of genes and proteins involved in later stages of B cell response. In summary, we show an influence of the gut microbiota on function of mucosal B2 B cells, involving mechanisms downstream of B cell activation and proliferation.

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author
publishing date
type
Contribution to journal
publication status
published
keywords
Animals, B-Lymphocytes, Biomarkers, Cell Proliferation, Colony Count, Microbial, Gastrointestinal Tract, Gene Expression Profiling, Gene Expression Regulation, Immunoglobulins, Intestinal Mucosa, Lymphocyte Activation, Metagenome, Mice, Peyer's Patches, Reproducibility of Results, Spleen
in
Molecular Immunology
volume
48
issue
9-10
pages
11 pages
publisher
Pergamon
external identifiers
  • Scopus:79954474577
ISSN
1872-9142
DOI
10.1016/j.molimm.2011.02.002
language
English
LU publication?
no
id
ffb2e16b-0b7d-4469-9740-3b5713810cc3
date added to LUP
2016-08-25 09:52:58
date last changed
2017-02-19 04:40:17
@article{ffb2e16b-0b7d-4469-9740-3b5713810cc3,
  abstract     = {<p>A complex interplay between the microbiota and the host immune system is evidenced to shape the immune system throughout life, but little is known about the microbial effect on key players of the adaptive immune system, the B2 B cells. In the presented study, we have evaluated the effect of commensal bacteria on B cell ontogeny and function, with the focus on B2 B cells of spleen and Peyer's patches. We have compared germ-free mice to mice that are exposed to a normal complex bacterial community from the day of birth and combined classical immunological assessment with advanced genome-wide expression profiling. Despite a preservation of all B cell subsets and phenotype, our results show that microbiota strongly impact mucosal B cell physiology and lead to higher serum Ig concentrations. We show that this microbial influence comprises downregulation of transcription factors involved in early B cell activation steps and upregulation of genes and proteins involved in later stages of B cell response. In summary, we show an influence of the gut microbiota on function of mucosal B2 B cells, involving mechanisms downstream of B cell activation and proliferation.</p>},
  author       = {Hansson, Jenny and Bosco, Nabil and Favre, Laurent and Raymond, Frederic and Oliveira, Manuel and Metairon, Sylviane and Mansourian, Robert and Blum, Stephanie and Kussmann, Martin and Benyacoub, Jalil},
  issn         = {1872-9142},
  keyword      = {Animals,B-Lymphocytes,Biomarkers,Cell Proliferation,Colony Count, Microbial,Gastrointestinal Tract,Gene Expression Profiling,Gene Expression Regulation,Immunoglobulins,Intestinal Mucosa,Lymphocyte Activation,Metagenome,Mice,Peyer's Patches,Reproducibility of Results,Spleen},
  language     = {eng},
  number       = {9-10},
  pages        = {101--1091},
  publisher    = {Pergamon},
  series       = {Molecular Immunology},
  title        = {Influence of gut microbiota on mouse B2 B cell ontogeny and function},
  url          = {http://dx.doi.org/10.1016/j.molimm.2011.02.002},
  volume       = {48},
  year         = {2011},
}