Характеристика імунофенотипів нормального ендометрію, еутопічного та ектопічного ендометрію жінок із ендометріозом і безпліддям.
(2018) In Morphologia 12(2). p.46-54- Abstract
- Background: Endometriosis is one of the most common causes of infertility in women. Despite extensive research on the subject, early-stage diagnosis remains challenging. Histologically, normal endometriosis does not differ from the eutopic endometrium, shifting the focus of investigation to the molecular level.
Objective: To assess the immunohistochemical markers ER, PGR, Ki-67, BCL-2, and MMP-9 in normal endometrial, eutopic, and ectopic endometrial tissue in women with endometriosis and infertility.
Methods: The study divided samples into three groups based on the expression of the investigated markers in glands (ER, PGR, Ki-67, BCL-2, MMP-9) and stroma (ER, PGR, Ki-67). Strict inclusion and exclusion criteria (clinical and/or... (More) - Background: Endometriosis is one of the most common causes of infertility in women. Despite extensive research on the subject, early-stage diagnosis remains challenging. Histologically, normal endometriosis does not differ from the eutopic endometrium, shifting the focus of investigation to the molecular level.
Objective: To assess the immunohistochemical markers ER, PGR, Ki-67, BCL-2, and MMP-9 in normal endometrial, eutopic, and ectopic endometrial tissue in women with endometriosis and infertility.
Methods: The study divided samples into three groups based on the expression of the investigated markers in glands (ER, PGR, Ki-67, BCL-2, MMP-9) and stroma (ER, PGR, Ki-67). Strict inclusion and exclusion criteria (clinical and/or morphological) were applied to organize these groups. Immunohistochemical staining was assessed in at least 10 fields of view per sample. Statistical analysis included nonparametric tests.
Results: Significant differences were observed between normal endometrium and both eutopic and ectopic endometrium for ER, PGR, and MMP-9 expression. Additionally, BCL-2 and Ki-67 showed significant differences between normal and eutopic endometrium (p < 0.05 for all markers). However, no statistically significant differences were found between eutopic and ectopic endometrium.
Conclusion: The combined use of ER, PGR, and MMP-9 immunohistochemical markers can aid in diagnosing endometriosis in women with infertility. The inclusion of BCL-2 and Ki-67 may further enhance diagnostic accuracy. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/ffeb76db-dcbe-4212-ad20-bf406e6a1dfa
- author
- Medvedev, Michail ; Shponka, Ihor ; Altanets, Olena and Skoryk, Valeriia LU
- alternative title
- Characteristic of immunophenotypes of normal endo-metrial, eutopic and ectopic endometrial tissue in women with endometriosis and infertility
- publishing date
- 2018-08-08
- type
- Contribution to journal
- publication status
- published
- keywords
- Eutopic and ectopic endometrium, Endometriosis, Infertility
- in
- Morphologia
- volume
- 12
- issue
- 2
- pages
- 9 pages
- publisher
- SE Dnipropetrovsk Medical Academy of Health Ministry of Ukraine
- ISSN
- 1997-9665
- DOI
- 10.26641/1997-9665.2018.2.46-54
- language
- Ukranian
- LU publication?
- no
- id
- ffeb76db-dcbe-4212-ad20-bf406e6a1dfa
- date added to LUP
- 2025-02-02 18:54:20
- date last changed
- 2025-02-03 10:01:11
@article{ffeb76db-dcbe-4212-ad20-bf406e6a1dfa, abstract = {{Background: Endometriosis is one of the most common causes of infertility in women. Despite extensive research on the subject, early-stage diagnosis remains challenging. Histologically, normal endometriosis does not differ from the eutopic endometrium, shifting the focus of investigation to the molecular level.<br/>Objective: To assess the immunohistochemical markers ER, PGR, Ki-67, BCL-2, and MMP-9 in normal endometrial, eutopic, and ectopic endometrial tissue in women with endometriosis and infertility.<br/>Methods: The study divided samples into three groups based on the expression of the investigated markers in glands (ER, PGR, Ki-67, BCL-2, MMP-9) and stroma (ER, PGR, Ki-67). Strict inclusion and exclusion criteria (clinical and/or morphological) were applied to organize these groups. Immunohistochemical staining was assessed in at least 10 fields of view per sample. Statistical analysis included nonparametric tests.<br/>Results: Significant differences were observed between normal endometrium and both eutopic and ectopic endometrium for ER, PGR, and MMP-9 expression. Additionally, BCL-2 and Ki-67 showed significant differences between normal and eutopic endometrium (p < 0.05 for all markers). However, no statistically significant differences were found between eutopic and ectopic endometrium.<br/>Conclusion: The combined use of ER, PGR, and MMP-9 immunohistochemical markers can aid in diagnosing endometriosis in women with infertility. The inclusion of BCL-2 and Ki-67 may further enhance diagnostic accuracy.}}, author = {{Medvedev, Michail and Shponka, Ihor and Altanets, Olena and Skoryk, Valeriia}}, issn = {{1997-9665}}, keywords = {{Eutopic and ectopic endometrium; Endometriosis; Infertility}}, language = {{ukr}}, month = {{08}}, number = {{2}}, pages = {{46--54}}, publisher = {{SE Dnipropetrovsk Medical Academy of Health Ministry of Ukraine}}, series = {{Morphologia}}, title = {{Характеристика імунофенотипів нормального ендометрію, еутопічного та ектопічного ендометрію жінок із ендометріозом і безпліддям.}}, url = {{http://dx.doi.org/10.26641/1997-9665.2018.2.46-54}}, doi = {{10.26641/1997-9665.2018.2.46-54}}, volume = {{12}}, year = {{2018}}, }