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Brain Tisue Response to Implanted SU-8

Trobäck, Anette LU (2011) EEM820 20111
Department of Biomedical Engineering
Abstract
Brain-machine interfaces are expected to play an important role in medicine – if a sustainable solution can be found – and so this study was performed to determine the biocompatibility of the photoresist SU-8 as well as a thin film needle design.This was done by histochemical staining with a collection of antibodies and mass spectrometry. The staining was performed on the perfused brains of four rats that had had cortical, contralateral implants for six weeks and of four rats with six week old stabwounds where the needle was retracted after two minutes. Histology showed a marked damage response in the controls as well as the implanted animals, implying that SU-8 affects the tissue in an unfavorable way, possibly by leaking into the... (More)
Brain-machine interfaces are expected to play an important role in medicine – if a sustainable solution can be found – and so this study was performed to determine the biocompatibility of the photoresist SU-8 as well as a thin film needle design.This was done by histochemical staining with a collection of antibodies and mass spectrometry. The staining was performed on the perfused brains of four rats that had had cortical, contralateral implants for six weeks and of four rats with six week old stabwounds where the needle was retracted after two minutes. Histology showed a marked damage response in the controls as well as the implanted animals, implying that SU-8 affects the tissue in an unfavorable way, possibly by leaking into the surrounds of the implant. It may also be as a result of fragmentation of the material at retraction causing an implantation-like control series. The hypothesis of leakage is supported by mass spectrometry using MALDI-TOF MS that shows a noticeable amount of material leakage with masses separate from the used matrix. The amplification of GFAP in the controls on the other hand implythat the needle design causes a greater damage than expected. Gathered results heavily suggest that SU-8 needs a more extensive study to pinpoint wherein the trouble lies before one would be able to discard it as a possibility for future implants. (Less)
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author
Trobäck, Anette LU
supervisor
organization
course
EEM820 20111
year
type
H2 - Master's Degree (Two Years)
subject
language
English
additional info
2011-06
id
2187834
date added to LUP
2011-10-24 13:21:38
date last changed
2014-10-08 14:47:20
@misc{2187834,
  abstract     = {Brain-machine interfaces are expected to play an important role in medicine – if a sustainable solution can be found – and so this study was performed to determine the biocompatibility of the photoresist SU-8 as well as a thin film needle design.This was done by histochemical staining with a collection of antibodies and mass spectrometry. The staining was performed on the perfused brains of four rats that had had cortical, contralateral implants for six weeks and of four rats with six week old stabwounds where the needle was retracted after two minutes. Histology showed a marked damage response in the controls as well as the implanted animals, implying that SU-8 affects the tissue in an unfavorable way, possibly by leaking into the surrounds of the implant. It may also be as a result of fragmentation of the material at retraction causing an implantation-like control series. The hypothesis of leakage is supported by mass spectrometry using MALDI-TOF MS that shows a noticeable amount of material leakage with masses separate from the used matrix. The amplification of GFAP in the controls on the other hand implythat the needle design causes a greater damage than expected. Gathered results heavily suggest that SU-8 needs a more extensive study to pinpoint wherein the trouble lies before one would be able to discard it as a possibility for future implants.},
  author       = {Trobäck, Anette},
  language     = {eng},
  note         = {Student Paper},
  title        = {Brain Tisue Response to Implanted SU-8},
  year         = {2011},
}