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Direct hollow fibre liquid phase membrane extraction and LC-MS determination of selective serotonin reuptake inhibitors in fish tissue

Engström, Henrik LU (2013) KEMZ06 20131
Department of Chemistry
Abstract
Selective serotonin reuptake inhibitors (SSRIs) have been found in waste water treatment plant effluents and surface water at detectable concentrations. Although the medical effects and side effects of pharmaceuticals and personal care products are investigated through safety and toxicology studies the potential environmental impacts are less studied, and information concerning ecotoxicological risks and the distribution in sludge, surface water and water living organisms is rather scarce.

In this thesis, sertraline (SER), fluoxetine (FLU) and its N-desmethyl metabolite norfluoxetine (norFLU) were chosen as model substances to develop a method for detection. Fish was chosen as the model matrix because fish is a top predator with... (More)
Selective serotonin reuptake inhibitors (SSRIs) have been found in waste water treatment plant effluents and surface water at detectable concentrations. Although the medical effects and side effects of pharmaceuticals and personal care products are investigated through safety and toxicology studies the potential environmental impacts are less studied, and information concerning ecotoxicological risks and the distribution in sludge, surface water and water living organisms is rather scarce.

In this thesis, sertraline (SER), fluoxetine (FLU) and its N-desmethyl metabolite norfluoxetine (norFLU) were chosen as model substances to develop a method for detection. Fish was chosen as the model matrix because fish is a top predator with possible high analyte concentrations because of biomagnification. Hollow fibre liquid-phase membrane extraction (HF-LPME) was used as the extraction, clean-up and enrichment technique and LC-MS was used to detect the SSRIs in fish muscle tissue.

This analytical method showed enrichment factors ranging 1500-1800 for fish samples and 3000-6300 for water samples. The R2-values of the linearity were 0.936, 0.990, 0.966 for norFLU, FLU and SER, respectively. The detection limits of the method for norFLU, FLU and SER were in the range of 130-280 ng L-1. The method was successfully applied to detect the analytes in exposed crucian carp; 1.7 μg g-1 FLU and 2.8 μg g-1 SER were found after exposure to a 51 μg L-1-mixture for 3 days. The FLU metabolite norFLU was not added in the exposure solution and it was not formed in detectable concentrations during the exposure. In unexposed crucian carp, none of the analytes were detected. The developed analytical method might be extended to estimate the distribution or the fate of norFLU, FLU and SER in other biota or human beings. (Less)
Popular Abstract (Swedish)
Spår av antidepressiva läkemedel i fisk
Läkemedel hjälper till att behandla och förebygga sjukdomar hos både djur och människor. Rigorösa utredningar krävs för att kartlägga deras effekter och bieffekter, men när de inte bryts ner helt i kroppen hamnar de i sjöar och vattendrag. Här är deras påverkan på djur och natur inte alls lika kartlagd.
Depression är bland de äldsta sjukdomarna kända för människan. Sedan den antidepressiva läkemedelsgruppen selektiva serotoninåterupptagshämmare (SSRI) introducerades behandlas även mildare former av depression med medicin. Detta har gjort SSRI-mediciner till de mest utskrivna psykofarmakan. Då forskning har visat att psykofarmaka inte helt bryts ner i kroppen är det av största vikt att hitta sätt... (More)
Spår av antidepressiva läkemedel i fisk
Läkemedel hjälper till att behandla och förebygga sjukdomar hos både djur och människor. Rigorösa utredningar krävs för att kartlägga deras effekter och bieffekter, men när de inte bryts ner helt i kroppen hamnar de i sjöar och vattendrag. Här är deras påverkan på djur och natur inte alls lika kartlagd.
Depression är bland de äldsta sjukdomarna kända för människan. Sedan den antidepressiva läkemedelsgruppen selektiva serotoninåterupptagshämmare (SSRI) introducerades behandlas även mildare former av depression med medicin. Detta har gjort SSRI-mediciner till de mest utskrivna psykofarmakan. Då forskning har visat att psykofarmaka inte helt bryts ner i kroppen är det av största vikt att hitta sätt att analysera denna typ av läkemedel i vattenlevande organismer.

Förutom låg koncentration är det alltid komplicerat att analysera ämnen i biologiska prover på grund av mängden andra, oönskade, ämnen som ofta skymmer sikten. Forskning har dock utvecklat metoder som är selektiva och högt anrikande av de önskade molekylerna.

En sådan teknik är hålfiberteknik, eller Hollow Fibre Liquid Phase Membrane Extraction (HF-LPME). Med hjälp av en tunn, porös slang (hålfiber), koncentreras de önskade molekylerna tusenfalt. Detta för också med sig att endast små mängder lösningsmedel behöver användas, vilket gör metoden miljövänlig.

I ett arbete utfört på Centrum för analys och syntes vid Lunds universitet, användes tekniken för att analysera SSRI i ruda från Revingehed. Arbetet visade att då fiskarna exponerades för SSRI i vattnet, kunde de antidepressiva läkemedlen även hittas i fiskvävnaden. (Less)
Please use this url to cite or link to this publication:
author
Engström, Henrik LU
supervisor
organization
course
KEMZ06 20131
year
type
H1 - Master's Degree (One Year)
subject
keywords
HF-LPME, LC-MS, SSRI, selective, fish, analytical chemistry, analytisk kemi
language
English
id
4124524
date added to LUP
2019-02-26 11:06:32
date last changed
2019-02-26 11:06:32
@misc{4124524,
  abstract     = {Selective serotonin reuptake inhibitors (SSRIs) have been found in waste water treatment plant effluents and surface water at detectable concentrations. Although the medical effects and side effects of pharmaceuticals and personal care products are investigated through safety and toxicology studies the potential environmental impacts are less studied, and information concerning ecotoxicological risks and the distribution in sludge, surface water and water living organisms is rather scarce.

In this thesis, sertraline (SER), fluoxetine (FLU) and its N-desmethyl metabolite norfluoxetine (norFLU) were chosen as model substances to develop a method for detection. Fish was chosen as the model matrix because fish is a top predator with possible high analyte concentrations because of biomagnification. Hollow fibre liquid-phase membrane extraction (HF-LPME) was used as the extraction, clean-up and enrichment technique and LC-MS was used to detect the SSRIs in fish muscle tissue.

This analytical method showed enrichment factors ranging 1500-1800 for fish samples and 3000-6300 for water samples. The R2-values of the linearity were 0.936, 0.990, 0.966 for norFLU, FLU and SER, respectively. The detection limits of the method for norFLU, FLU and SER were in the range of 130-280 ng L-1. The method was successfully applied to detect the analytes in exposed crucian carp; 1.7 μg g-1 FLU and 2.8 μg g-1 SER were found after exposure to a 51 μg L-1-mixture for 3 days. The FLU metabolite norFLU was not added in the exposure solution and it was not formed in detectable concentrations during the exposure. In unexposed crucian carp, none of the analytes were detected. The developed analytical method might be extended to estimate the distribution or the fate of norFLU, FLU and SER in other biota or human beings.},
  author       = {Engström, Henrik},
  keyword      = {HF-LPME,LC-MS,SSRI,selective,fish,analytical chemistry,analytisk kemi},
  language     = {eng},
  note         = {Student Paper},
  title        = {Direct hollow fibre liquid phase membrane extraction and LC-MS determination of selective serotonin reuptake inhibitors in fish tissue},
  year         = {2013},
}