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Development of an analytical method for isolation and identification of phenolic compounds in human urine samples

Kaddoura, Wadia LU (2013) KEMR16 20131
Department of Chemistry
Abstract
Polyphenols are a group of compounds that are widely spread in the plant kingdom. These compounds are of great interest for researchers because of their antioxidant properties that are important for both plant life and human health. Sample preparation is a crucial step for the determination of phenolic compounds and their metabolites in biological samples. Methods for isolation and preconcentration of these compounds from human urine matrices by using C-18 HyperSepTM tips associated with micro-solid phase extraction (micro-SPE) technique have been tested in this study. A broad range of polyphenols, namely; phenolic acid, flavonol and anthocyanin standards were separated and identified by Reversed Phase High Temperature Liquid... (More)
Polyphenols are a group of compounds that are widely spread in the plant kingdom. These compounds are of great interest for researchers because of their antioxidant properties that are important for both plant life and human health. Sample preparation is a crucial step for the determination of phenolic compounds and their metabolites in biological samples. Methods for isolation and preconcentration of these compounds from human urine matrices by using C-18 HyperSepTM tips associated with micro-solid phase extraction (micro-SPE) technique have been tested in this study. A broad range of polyphenols, namely; phenolic acid, flavonol and anthocyanin standards were separated and identified by Reversed Phase High Temperature Liquid Chromatography (RP-HTLC) coupled to Ultra Violet/ Visible Light (UV/VIS) and PhotoDiode Array detector (PDA). The RP-HTLC-method has been validated; the limit of detection for the phenolic acids ranged between 0.15 and 0.76 μgmL-1 and the calibration curves showed excellent linearity all with R2 > 0.99. The precision of the overall analytical procedure was estimated as the relative standard deviations (RSD %) through repeating the analysis seven times in one day. The RSDs for all retention times were < 1 % and for all peak areas, < 10 %. The Micro-SPE methods studied showed poor recovery which was at best 25 % recovery of vanillic acid and 27 % recovery of 3, 4-dihydroxyphenylacetic acid. (Less)
Popular Abstract
An analytical method for isolation and identification of phenolic compounds in human urine samples
Polyphenols are a group of compounds that play an important role in both plant life and human health. This importance arises from their antioxidant properties. These antioxidants spread widely in the plant kingdom especially fruits, vegetables and spices and are believed to lower the risk of many chronic diseases and disorders such as cancer, heart disease, stroke, Alzheimer’s disease and Rheumatoid arthritis, in addition to their role in anti-aging and to healthy look. Antioxidants neutralize the reactive radicals produced inside and outside our bodies and prevent them from damaging our cells. Because of these effects scientists and... (More)
An analytical method for isolation and identification of phenolic compounds in human urine samples
Polyphenols are a group of compounds that play an important role in both plant life and human health. This importance arises from their antioxidant properties. These antioxidants spread widely in the plant kingdom especially fruits, vegetables and spices and are believed to lower the risk of many chronic diseases and disorders such as cancer, heart disease, stroke, Alzheimer’s disease and Rheumatoid arthritis, in addition to their role in anti-aging and to healthy look. Antioxidants neutralize the reactive radicals produced inside and outside our bodies and prevent them from damaging our cells. Because of these effects scientists and researchers work actively and continuously in reporting and reviewing scientific literature considering the types and quantities of antioxidants that are needed for healthy life. To achieve these objectives modern and sensitive instruments are used. In this work a broad range of phenolic compounds were separated and identified by using a separation technique coupled to a detector to separate and detect these compounds with good accuracy and at reasonable time. Method for isolation of phenolic compounds from complex matrices like human urine was also developed to meet the objective of this project. This method showed poor recovery and that is due to both complexity of the these matrices and the technique used for isolation. (Less)
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author
Kaddoura, Wadia LU
supervisor
organization
course
KEMR16 20131
year
type
H2 - Master's Degree (Two Years)
subject
keywords
analytisk kemi, analytical chemistry
language
English
id
4646162
date added to LUP
2014-09-12 16:20:36
date last changed
2014-09-12 16:20:36
@misc{4646162,
  abstract     = {Polyphenols are a group of compounds that are widely spread in the plant kingdom. These compounds are of great interest for researchers because of their antioxidant properties that are important for both plant life and human health. Sample preparation is a crucial step for the determination of phenolic compounds and their metabolites in biological samples. Methods for isolation and preconcentration of these compounds from human urine matrices by using C-18 HyperSepTM tips associated with micro-solid phase extraction (micro-SPE) technique have been tested in this study. A broad range of polyphenols, namely; phenolic acid, flavonol and anthocyanin standards were separated and identified by Reversed Phase High Temperature Liquid Chromatography (RP-HTLC) coupled to Ultra Violet/ Visible Light (UV/VIS) and PhotoDiode Array detector (PDA). The RP-HTLC-method has been validated; the limit of detection for the phenolic acids ranged between 0.15 and 0.76 μgmL-1 and the calibration curves showed excellent linearity all with R2 > 0.99. The precision of the overall analytical procedure was estimated as the relative standard deviations (RSD %) through repeating the analysis seven times in one day. The RSDs for all retention times were < 1 % and for all peak areas, < 10 %. The Micro-SPE methods studied showed poor recovery which was at best 25 % recovery of vanillic acid and 27 % recovery of 3, 4-dihydroxyphenylacetic acid.},
  author       = {Kaddoura, Wadia},
  keyword      = {analytisk kemi,analytical chemistry},
  language     = {eng},
  note         = {Student Paper},
  title        = {Development of an analytical method for isolation and identification of phenolic compounds in human urine samples},
  year         = {2013},
}