The gut microbial composition in humans in hot occupational settings and the effects of drinking buttermilk
(2015) KLGM01 20151Food Technology and Nutrition (M.Sc.)
- Abstract
- The principal aims of the present study were to examine the latent heat stress relieving capacity of drinking buttermilk and to observe the human gut microbiota in hot occupational condition. A human crossover intervention study with 12 volunteers was studied, each of them performed 3 hours of physical work in a heat chamber. During the working period, the volunteers were given buttermilk, water or no liquid. Rectal and saliva samples were obtained after and during 3 hours of physical work.
The results showed no significant differences in diversity indices of three treatment groups, we detected, by means of salivary cortisol level analysis, significantly differences between the Buttermilk and Dehydrated groups, as well as Water and... (More) - The principal aims of the present study were to examine the latent heat stress relieving capacity of drinking buttermilk and to observe the human gut microbiota in hot occupational condition. A human crossover intervention study with 12 volunteers was studied, each of them performed 3 hours of physical work in a heat chamber. During the working period, the volunteers were given buttermilk, water or no liquid. Rectal and saliva samples were obtained after and during 3 hours of physical work.
The results showed no significant differences in diversity indices of three treatment groups, we detected, by means of salivary cortisol level analysis, significantly differences between the Buttermilk and Dehydrated groups, as well as Water and Dehydrated groups at the last time-point (P = 0.017). When it comes to qPCR results, Lactobacillus and Enterobacteriaceae were detected in 9 (75%) and 12 (100%) subjects, with the medians of 4.039 (Buttermilk), 4.02 (Dehydrated), 4.081 (Water) and 7.407 (Buttermilk), 6.763 (Dehydrated), 7.64 (Water) log 16S rRNA gene copies/g rectal samples respectively.
The PCA score and scatter plots indicated that the microbiota differed widely between individuals with regard to both composition and diversity. While the PLS score and loading scatter plots based on cortisol, core temperature and T-RFLP data explicated that the observations separated in groups which representing three treatments of same subject. However, the relationship between the stress indicators (core temperature and salivary cortisol level) and the gut microbial diversity was not clearly shown. (Less) - Popular Abstract
- In our gastrointestinal tract lives millions of bacteria and these living microbes are called “microbiota”. You may have not noticed that these living creatures are so important to our body that a lot of diseases such as obesity, type 2 diabetes, inflammatory bowel diseases and even psychological diseases are associated tightly with them. Moreover, a growing body of evidence has proved that interaction between microbiota and human bodies is essential for the development of nervous system and regulation of neural functions.
Heat stress is common issue in workers such as constructors, minors, soldiers and fire fighters across warm climate counties. When the ambient temperature reaches or exceeds 38 degree Celsius, it can deteriorate... (More) - In our gastrointestinal tract lives millions of bacteria and these living microbes are called “microbiota”. You may have not noticed that these living creatures are so important to our body that a lot of diseases such as obesity, type 2 diabetes, inflammatory bowel diseases and even psychological diseases are associated tightly with them. Moreover, a growing body of evidence has proved that interaction between microbiota and human bodies is essential for the development of nervous system and regulation of neural functions.
Heat stress is common issue in workers such as constructors, minors, soldiers and fire fighters across warm climate counties. When the ambient temperature reaches or exceeds 38 degree Celsius, it can deteriorate efficiency and productivity and even threaten survival. In this study, we studied a dairy product called “buttermilk”, which is a traditional dairy drink in summer to deal with the heat stress across the warm climate countries. It is a diluted form of plain yogurt and some spices, such as curry, ginger etc., are usually added according a traditional recipe in India.
In order to find the possible effects of drinking buttermilk on heat stress. We studied the response of the microbiota to buttermilk, water and no liquid ingestion during 3 hours’ physical work in a heat chamber (37 degree Celsius). The level of stress marker, cortisol (a hormone), was also measured during the test.
We found significant differences in cortisol levels between Buttermilk and Water treatment group, as well as Water and No liquid group at the end of test. The significantly differences between treatment groups indicate that buttermilk had better relieving effect on heat stress compared with water. As for the water and dehydrated group, water was more helpful dealing with heat stress, which was a very obviously answer.
When it comes to the microbiota, although no significant differences were found among three treatments, we still observed that there was a relation between stress and microbiota by the means of relating core temperature and microbiota, as well as cortisol level and microbiota. In a word, the test confirmed the theory that there is a relation between stress and gut microbiota. However, more studies have to be done to get further proof. On the other hand, the human trial is very complex system and many factors including personal and environmental perspectives have to be considered in order to get creditable results. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/8055578
- author
- Song, Danping LU
- supervisor
- organization
- course
- KLGM01 20151
- year
- 2015
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- Gut microbiota, Buttermilk, heat stress, livsmedelsteknologi, food technology
- language
- English
- id
- 8055578
- date added to LUP
- 2015-10-15 16:25:08
- date last changed
- 2015-10-21 08:57:41
@misc{8055578,
abstract = {{The principal aims of the present study were to examine the latent heat stress relieving capacity of drinking buttermilk and to observe the human gut microbiota in hot occupational condition. A human crossover intervention study with 12 volunteers was studied, each of them performed 3 hours of physical work in a heat chamber. During the working period, the volunteers were given buttermilk, water or no liquid. Rectal and saliva samples were obtained after and during 3 hours of physical work.
The results showed no significant differences in diversity indices of three treatment groups, we detected, by means of salivary cortisol level analysis, significantly differences between the Buttermilk and Dehydrated groups, as well as Water and Dehydrated groups at the last time-point (P = 0.017). When it comes to qPCR results, Lactobacillus and Enterobacteriaceae were detected in 9 (75%) and 12 (100%) subjects, with the medians of 4.039 (Buttermilk), 4.02 (Dehydrated), 4.081 (Water) and 7.407 (Buttermilk), 6.763 (Dehydrated), 7.64 (Water) log 16S rRNA gene copies/g rectal samples respectively.
The PCA score and scatter plots indicated that the microbiota differed widely between individuals with regard to both composition and diversity. While the PLS score and loading scatter plots based on cortisol, core temperature and T-RFLP data explicated that the observations separated in groups which representing three treatments of same subject. However, the relationship between the stress indicators (core temperature and salivary cortisol level) and the gut microbial diversity was not clearly shown.}},
author = {{Song, Danping}},
language = {{eng}},
note = {{Student Paper}},
title = {{The gut microbial composition in humans in hot occupational settings and the effects of drinking buttermilk}},
year = {{2015}},
}