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Development of a high throuput global lipidomics platform and its applicationin studies on metabolic disease

Armeni, Marina LU (2016) KEMR36 20161
Department of Chemistry
Abstract
Current drawback in mass spectrometry-based lipidomics is the absence of a global and comprehensive lipid mass spectra library. Thus, the construction of such library was necessary, serving as a tool in different research projects. A liquid-liquid extraction (LLE) method for extraction of lipids from bovine serum and human blood plasma has been developed. Moreover, two different approaches were used for lipid identification with two different mass analyzers (a) Orbitrap for untargeted analysis and (b) quadrupole Time-Of-Flight (qTOF) for targeted analysis. The final library includes more than 226 lipids of different classes. This library has been used in a screening of columns for supercritical fluid chromatography based lipidomics and... (More)
Current drawback in mass spectrometry-based lipidomics is the absence of a global and comprehensive lipid mass spectra library. Thus, the construction of such library was necessary, serving as a tool in different research projects. A liquid-liquid extraction (LLE) method for extraction of lipids from bovine serum and human blood plasma has been developed. Moreover, two different approaches were used for lipid identification with two different mass analyzers (a) Orbitrap for untargeted analysis and (b) quadrupole Time-Of-Flight (qTOF) for targeted analysis. The final library includes more than 226 lipids of different classes. This library has been used in a screening of columns for supercritical fluid chromatography based lipidomics and will be used in multiple research projects, including studies of the metabolic response to a multifunctional diet. (Less)
Popular Abstract
Development of a high, throughput mass spectral library for lipids and its application on metabolic disease.
A drawback in mass spectrometry-based lipidomics concerns the available information of the databases. Current online databases in lipidomics are inconsistent in content and if someone wants to examine a large amount of lipids, many online databases are utilized since there is no single database which contains all the lipids. That makes data analyses a time consuming process. For all these reasons, the construction of a library that contains as many as possible lipids is an essential tool for conducting research.
Lipidomics has emerged due to the realization of importance of lipidome. Lipids are vital for diverse processes in all... (More)
Development of a high, throughput mass spectral library for lipids and its application on metabolic disease.
A drawback in mass spectrometry-based lipidomics concerns the available information of the databases. Current online databases in lipidomics are inconsistent in content and if someone wants to examine a large amount of lipids, many online databases are utilized since there is no single database which contains all the lipids. That makes data analyses a time consuming process. For all these reasons, the construction of a library that contains as many as possible lipids is an essential tool for conducting research.
Lipidomics has emerged due to the realization of importance of lipidome. Lipids are vital for diverse processes in all living organisms, and play a key role in many diseases. Lipidomics is a research field dedicated to the global study of the lipidome, i.e. the study of all lipids in a biological system. For these analyses lipid libraries are important. Thus the aim of this project was to develop a library containing the experimental and theoretical masses of as many lipids as possible, their respective elution times and their peaks on MS2 spectra. To format this library a method development for lipid extraction and lipid analysis with HPLC-MS and UHPLC-MS instrumentation was conducted.
The first step of the workflow is the development of a protocol for extraction of lipids from bovine serum and human blood plasma. Then a UPLC-MS method is developed to putatively identify the lipids followed by an UPLC-MS/MS method development aiming the structural characterization of lipids. In this phase the masses of the most abundant lipids in human blood plasma are chosen to be triggered (targeted analysis).
The final result is the construction of a library of 226 identified lipids, containing information about their theoretical and experimental masses, their retention times, matrix, mode of the ion source, their peaks. Reference information are also included in But this only the beginning; the library will be expanded with more lipids.
Supervisors: Peter Spégel, Claudia Balderas, Said Al Hamimi (Less)
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author
Armeni, Marina LU
supervisor
organization
course
KEMR36 20161
year
type
H2 - Master's Degree (Two Years)
subject
keywords
analytisk kemi, analytical chemistry, lipid extraction, lipid library, Mass Spectrometry, lipidomics
language
English
id
8885329
date added to LUP
2016-07-01 14:12:51
date last changed
2016-07-01 14:12:51
@misc{8885329,
  abstract     = {Current drawback in mass spectrometry-based lipidomics is the absence of a global and comprehensive lipid mass spectra library. Thus, the construction of such library was necessary, serving as a tool in different research projects. A liquid-liquid extraction (LLE) method for extraction of lipids from bovine serum and human blood plasma has been developed. Moreover, two different approaches were used for lipid identification with two different mass analyzers (a) Orbitrap for untargeted analysis and (b) quadrupole Time-Of-Flight (qTOF) for targeted analysis. The final library includes more than 226 lipids of different classes. This library has been used in a screening of columns for supercritical fluid chromatography based lipidomics and will be used in multiple research projects, including studies of the metabolic response to a multifunctional diet.},
  author       = {Armeni, Marina},
  keyword      = {analytisk kemi,analytical chemistry,lipid extraction,lipid library,Mass Spectrometry,lipidomics},
  language     = {eng},
  note         = {Student Paper},
  title        = {Development of a high throuput global lipidomics platform and its applicationin studies on metabolic disease},
  year         = {2016},
}