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TOXICOLOGICAL EFFECTS OF SILVER AND GOLD NANOPARTICLES IN THE RETINA (in vivo)

Abdulla, Nada LU (2017) KEMT10 20162
Department of Chemistry
Abstract
Abstract
The retina is the inner most layer of the eye. It is responsible for conversion of light into an electrical signal that pass through the fibers of the optic nerve to the visual centers in the brain. Most of the neo-vascular retinal diseases lead to legal vision loss. Silver and gold nanoparticles have got a great interest in ocular research due to their anti-bacterial effects and their ability to inhibit retinal neovascularization and pass retinal blood barrier. Here we studied toxicological effects (apoptosis and microglial activation) of silver and gold nanoparticles, 20 and 80 nm in adult mouse retina after different times of intravitreal injection. Different staining methods were used, includingTUNEL staining, Iba1 and ED1... (More)
Abstract
The retina is the inner most layer of the eye. It is responsible for conversion of light into an electrical signal that pass through the fibers of the optic nerve to the visual centers in the brain. Most of the neo-vascular retinal diseases lead to legal vision loss. Silver and gold nanoparticles have got a great interest in ocular research due to their anti-bacterial effects and their ability to inhibit retinal neovascularization and pass retinal blood barrier. Here we studied toxicological effects (apoptosis and microglial activation) of silver and gold nanoparticles, 20 and 80 nm in adult mouse retina after different times of intravitreal injection. Different staining methods were used, includingTUNEL staining, Iba1 and ED1 double immunostaining. Also, autometallography-based silver staining was used to detect Au and Ag nanoparticles (NPs). Most of the apoptotic cells were detected in the outer nuclear layer (ONL), AuNPs 20nm had the highest toxic effect followed by AuNPs, 80nm. It was also found AuNPs 20 nm cause the highest inflammation effect which cause degeneration of microglial cells at the late activation state. (Less)
Popular Abstract
Small particles are everywhere!
In our life style, we daily use many products like sun screen, anti -scratch frying pan, make up and pacifiers for babies. All of these products contain tiny small particles called nanoparticles. They are defined as sub 100 nm structures. There are different types of nanoparticles depending on the material source. Some nanoparticles are used as a vehicle to deliver drugs to cells (e.g. cancer cells). Due to their large surface area relative to their volume, they can carry large amount of drugs. Gold (Au) nanoparticles have many applications in the medical field especially in the cancer treatment, Silver (Ag) nanoparticles also got great attention as they have the ability to kill the bacteria. Besides having... (More)
Small particles are everywhere!
In our life style, we daily use many products like sun screen, anti -scratch frying pan, make up and pacifiers for babies. All of these products contain tiny small particles called nanoparticles. They are defined as sub 100 nm structures. There are different types of nanoparticles depending on the material source. Some nanoparticles are used as a vehicle to deliver drugs to cells (e.g. cancer cells). Due to their large surface area relative to their volume, they can carry large amount of drugs. Gold (Au) nanoparticles have many applications in the medical field especially in the cancer treatment, Silver (Ag) nanoparticles also got great attention as they have the ability to kill the bacteria. Besides having many treatment applications in the medical field, they also have side effects so it is a double-edged weapon. Many factors control these effects such as the particles’ size, type, concentration, the administration route, the nature of the target etc. One of the most sensitive organs that are exposed daily to these particles is the eye. Exposure to nanoparticles can have different side effects, such as cell death and inflammation. Therefore, we here studied effects of Ag- and Au nanoparticles on the retina in vivo, by analyzing cell death and inflammation using specific stainings. By studying the side effects of nanoparticles, more progress in the medical field will be carried out. In this study, we found that 20 nm Au nanoparticles had the highest toxic effect while no effect was found for Ag nanoparticles. (Less)
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author
Abdulla, Nada LU
supervisor
organization
course
KEMT10 20162
year
type
H1 - Master's Degree (One Year)
subject
keywords
Silver and Gold Nanoparticles, Retina, protein science, proteinvetenskap
language
English
id
8925143
date added to LUP
2018-02-01 11:47:24
date last changed
2018-02-01 11:47:24
@misc{8925143,
  abstract     = {Abstract
The retina is the inner most layer of the eye. It is responsible for conversion of light into an electrical signal that pass through the fibers of the optic nerve to the visual centers in the brain. Most of the neo-vascular retinal diseases lead to legal vision loss. Silver and gold nanoparticles have got a great interest in ocular research due to their anti-bacterial effects and their ability to inhibit retinal neovascularization and pass retinal blood barrier. Here we studied toxicological effects (apoptosis and microglial activation) of silver and gold nanoparticles, 20 and 80 nm in adult mouse retina after different times of intravitreal injection. Different staining methods were used, includingTUNEL staining, Iba1 and ED1 double immunostaining. Also, autometallography-based silver staining was used to detect Au and Ag nanoparticles (NPs). Most of the apoptotic cells were detected in the outer nuclear layer (ONL), AuNPs 20nm had the highest toxic effect followed by AuNPs, 80nm. It was also found AuNPs 20 nm cause the highest inflammation effect which cause degeneration of microglial cells at the late activation state.},
  author       = {Abdulla, Nada},
  keyword      = {Silver and Gold Nanoparticles,Retina,protein science,proteinvetenskap},
  language     = {eng},
  note         = {Student Paper},
  title        = {TOXICOLOGICAL EFFECTS OF SILVER AND GOLD NANOPARTICLES IN THE RETINA (in vivo)},
  year         = {2017},
}