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Examination of bioactive compounds in the liver and venom sac of the ratfish C. monstrosa

Ramm, Ingrid LU (2017) KLGL01 20162
Food Science
Abstract
The deep sea fish C. monstrosa (known as the rat fish) is a common bycatch of shrimp trawling. The rat fish carries on its back a vigorous venom spine. Some of the most toxic organisms on this planet are marine. The active substances differ in the venoms of the toxic organism, but the active substances are in many cases peptides. Natural peptides found in venoms have recently become more common candidates in the search of drug leads. The liver oil from C. monstrosa has in the area around Skagerrak traditionally been used a natural remedy. The liver oil is believed to have a wound healing and analgesic effects. The active substances in the liver and venom of C. monstrosa are therefore of medical interest. To examine the bioactivities of the... (More)
The deep sea fish C. monstrosa (known as the rat fish) is a common bycatch of shrimp trawling. The rat fish carries on its back a vigorous venom spine. Some of the most toxic organisms on this planet are marine. The active substances differ in the venoms of the toxic organism, but the active substances are in many cases peptides. Natural peptides found in venoms have recently become more common candidates in the search of drug leads. The liver oil from C. monstrosa has in the area around Skagerrak traditionally been used a natural remedy. The liver oil is believed to have a wound healing and analgesic effects. The active substances in the liver and venom of C. monstrosa are therefore of medical interest. To examine the bioactivities of the active compounds in the liver and venom sac of C. monstrosa a small scale peptide extraction was first performed, followed by an Artemia assay and an bacteria assay. To obtain more information of the extracted compounds, a LC-MS experiment was performed. The Artemia and bacteria assay showed that the extracted compounds have a lethal effect on A. salina and S.aureus. The Artemia assay also showed that the extracted compound also had an effect on the mortality of the A. salina. The LC-MS result showed that the extracted material contained peptides. The peptide sequences were not determined but the LC-MS result generated a list of proposed peptide sequences. Some of the proposed peptide sequences were found in all extracted samples, both from the liver and the venom sac. (Less)
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author
Ramm, Ingrid LU
supervisor
organization
course
KLGL01 20162
year
type
M2 - Bachelor Degree
subject
keywords
C. monstrosa, bioactive compounds, natural peptides, pharmaceutical technology, läkemedelsteknologi
language
English
id
8925542
date added to LUP
2019-04-08 14:51:56
date last changed
2019-04-08 14:51:56
@misc{8925542,
  abstract     = {The deep sea fish C. monstrosa (known as the rat fish) is a common bycatch of shrimp trawling. The rat fish carries on its back a vigorous venom spine. Some of the most toxic organisms on this planet are marine. The active substances differ in the venoms of the toxic organism, but the active substances are in many cases peptides. Natural peptides found in venoms have recently become more common candidates in the search of drug leads. The liver oil from C. monstrosa has in the area around Skagerrak traditionally been used a natural remedy. The liver oil is believed to have a wound healing and analgesic effects. The active substances in the liver and venom of C. monstrosa are therefore of medical interest. To examine the bioactivities of the active compounds in the liver and venom sac of C. monstrosa a small scale peptide extraction was first performed, followed by an Artemia assay and an bacteria assay. To obtain more information of the extracted compounds, a LC-MS experiment was performed. The Artemia and bacteria assay showed that the extracted compounds have a lethal effect on A. salina and S.aureus. The Artemia assay also showed that the extracted compound also had an effect on the mortality of the A. salina. The LC-MS result showed that the extracted material contained peptides. The peptide sequences were not determined but the LC-MS result generated a list of proposed peptide sequences. Some of the proposed peptide sequences were found in all extracted samples, both from the liver and the venom sac.},
  author       = {Ramm, Ingrid},
  keyword      = {C. monstrosa,bioactive compounds,natural peptides,pharmaceutical technology,läkemedelsteknologi},
  language     = {eng},
  note         = {Student Paper},
  title        = {Examination of bioactive compounds in the liver and venom sac of the ratfish C. monstrosa},
  year         = {2017},
}