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Starch based formulation for colon delivery of probiotics

Roxling, Elsa LU (2019) KLGM15 20182
Food Technology and Nutrition (M.Sc.)
Abstract
The interest in probiotics has grown in the last few years. In addition to being effective in treating and preventing acute diarrhea, there are indications that it might be possible to use probiotics to prevent other diseases such as colon cancer. The challenge with probiotics is that for it to have any effect, enough viable bacteria must reach the colon. To deliver live probiotics to the colon, it is necessary to protect the bacteria in some manner as most of them cannot survive the harsh environment in the gastrointestinal tract. A formulation consisting of starch microspheres with the bacteria encapsulated in the starch matrix was investigated. Starch microspheres were made by an aqueous two-phase system, with dissolved starch as the... (More)
The interest in probiotics has grown in the last few years. In addition to being effective in treating and preventing acute diarrhea, there are indications that it might be possible to use probiotics to prevent other diseases such as colon cancer. The challenge with probiotics is that for it to have any effect, enough viable bacteria must reach the colon. To deliver live probiotics to the colon, it is necessary to protect the bacteria in some manner as most of them cannot survive the harsh environment in the gastrointestinal tract. A formulation consisting of starch microspheres with the bacteria encapsulated in the starch matrix was investigated. Starch microspheres were made by an aqueous two-phase system, with dissolved starch as the internal phase and dissolved polyethylene glycol (PEG) as the external phase. To crystallize the starch, the samples were incubated at 6 °C followed by 37 °C. Two different average molecular weight PEGs and several different PEG concentrations, as well as three different acid hydrolyzed waxy starches were investigated to find the best combination. 13 wt% PEG 2 000 000 and acid hydrolyzed waxy maize starch was chosen to produce microspheres containing microorganisms. Microorganisms of the type strain Lactobacillus reuteri DSM 20016 were successfully encapsulated in microspheres; however the freeze drying process of the bacteria prior to encapsulation killed most of them. The microspheres were analyzed with a simulated in vitro intestinal digestion. The work done in this project was very early explorative phase and a lot of work is needed to optimize the production process, and the freeze drying of the microorganisms. (Less)
Abstract (Swedish)
Intresset för probiotika har vuxit de senaste åren. Förutom att vara ha en förebyggande effekt och vara en effektiv behandling mot akut diarré, finns det forskning som indikerar att probiotika kanske kan användas för att förebygga andra sjukdomar, t.ex. tjocktarmscancer. Utmaningen med probiotika är att för att den ska ha någon effekt, krävs det att tillräckligt många levande bakterier når tjocktarmen. För att göra det, måste bakterierna skyddas på något sätt eftersom de flesta inte överlever de tuffa miljöer som finns i mag- och tarmkanalen. En formulering som består av bakterier inkapslade i stärkelsemikrosfärer undersöktes. Stärkelsemikrosfärer var tillverkade med ett vattenbaserat två-fas-system, med upplöst stärkelse i den inre fasen... (More)
Intresset för probiotika har vuxit de senaste åren. Förutom att vara ha en förebyggande effekt och vara en effektiv behandling mot akut diarré, finns det forskning som indikerar att probiotika kanske kan användas för att förebygga andra sjukdomar, t.ex. tjocktarmscancer. Utmaningen med probiotika är att för att den ska ha någon effekt, krävs det att tillräckligt många levande bakterier når tjocktarmen. För att göra det, måste bakterierna skyddas på något sätt eftersom de flesta inte överlever de tuffa miljöer som finns i mag- och tarmkanalen. En formulering som består av bakterier inkapslade i stärkelsemikrosfärer undersöktes. Stärkelsemikrosfärer var tillverkade med ett vattenbaserat två-fas-system, med upplöst stärkelse i den inre fasen och upplöst polyetylenglykol (PEG) i den yttre fasen. För att kristallisera stärkelsen inkuberades proverna i 6 °C, följt av 37 °C. Två sorters PEG med olika genomsnittlig molekylvikt och flera olika PEG-koncentrationer, samt tre olika syrahydrolyserade stärkelser med hög andel amylopektin, testades för att hitta den bästa kombinationen. 13 wt% PEG 2 000 000 och syrahydrolyserad hög amylopektin majsstärkelse valdes för att producera mikrosfärer som innehöll bakterier. Mikroorganismer av typstammen Lactobacillus reuteri DSM 20016 inkapslades framgångsrikt i mikrosfärer; men frystorkningen av bakterierna innan inkapslingen dödade de flesta av dem. Mikrosfärerna var analyserade med hjälp av en in vitro-simulering av tunntarmen. Arbetet som utfördes i detta projekt var väldigt initialt och mycket arbete med att optimera produktionsprocessen, och frystorkningen av bakterierna är nödvändigt. (Less)
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author
Roxling, Elsa LU
supervisor
organization
course
KLGM15 20182
year
type
H2 - Master's Degree (Two Years)
subject
keywords
läkemedelsteknologi, pharmaceutical technology
language
English
id
8967391
date added to LUP
2019-04-02 09:37:52
date last changed
2019-04-02 09:37:52
@misc{8967391,
  abstract     = {The interest in probiotics has grown in the last few years. In addition to being effective in treating and preventing acute diarrhea, there are indications that it might be possible to use probiotics to prevent other diseases such as colon cancer. The challenge with probiotics is that for it to have any effect, enough viable bacteria must reach the colon. To deliver live probiotics to the colon, it is necessary to protect the bacteria in some manner as most of them cannot survive the harsh environment in the gastrointestinal tract. A formulation consisting of starch microspheres with the bacteria encapsulated in the starch matrix was investigated. Starch microspheres were made by an aqueous two-phase system, with dissolved starch as the internal phase and dissolved polyethylene glycol (PEG) as the external phase. To crystallize the starch, the samples were incubated at 6 °C followed by 37 °C. Two different average molecular weight PEGs and several different PEG concentrations, as well as three different acid hydrolyzed waxy starches were investigated to find the best combination. 13 wt% PEG 2 000 000 and acid hydrolyzed waxy maize starch was chosen to produce microspheres containing microorganisms. Microorganisms of the type strain Lactobacillus reuteri DSM 20016 were successfully encapsulated in microspheres; however the freeze drying process of the bacteria prior to encapsulation killed most of them. The microspheres were analyzed with a simulated in vitro intestinal digestion. The work done in this project was very early explorative phase and a lot of work is needed to optimize the production process, and the freeze drying of the microorganisms.},
  author       = {Roxling, Elsa},
  keyword      = {läkemedelsteknologi,pharmaceutical technology},
  language     = {eng},
  note         = {Student Paper},
  title        = {Starch based formulation for colon delivery of probiotics},
  year         = {2019},
}