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Develop a method to detect nanoparticles in tap water using AF4

Zhang, Lingping LU (2019) KLGM01 20191
Food Technology and Nutrition (M.Sc.)
Abstract
40 L tap water was ultra-filtered by M10 lab unit using 10 kDa regenerated membrane and 800 nm syringe filter to get the NPs. The filter NPs are then separated and characterized using asymmetrical flow field-flow fractionation with coupled multi angle light scattering (AF4-MALS). Calibration curve for determining the size distribution is made by polystyrene latex bead (20, 60, 100, 300, and 500 nm) to calculate the size distribution of the NPs in the tap water samples with the size range between 20 to 800 nm. The mean diameter of the NPs in tap water is 177.6 nm and most of them are around 100 to 300 nm. The different kinds of metal ions were analyzed for three different size fraction ranges (20 to 100 nm, 100 to 200 nm and 200 to 300 nm)... (More)
40 L tap water was ultra-filtered by M10 lab unit using 10 kDa regenerated membrane and 800 nm syringe filter to get the NPs. The filter NPs are then separated and characterized using asymmetrical flow field-flow fractionation with coupled multi angle light scattering (AF4-MALS). Calibration curve for determining the size distribution is made by polystyrene latex bead (20, 60, 100, 300, and 500 nm) to calculate the size distribution of the NPs in the tap water samples with the size range between 20 to 800 nm. The mean diameter of the NPs in tap water is 177.6 nm and most of them are around 100 to 300 nm. The different kinds of metal ions were analyzed for three different size fraction ranges (20 to 100 nm, 100 to 200 nm and 200 to 300 nm) by inductively coupled plasma mass spectroscopy (ICP-MS) and optical emission spectrometry (ICP-OES) techniques. As a result, Fe, Cu, Ni, and Ag were not detectable and Ca, Si, Al, Pb and Au have the high concentration in the 20 to 100 nm size range which has the highest possibility of toxicity. Zn and Pb are over the limit compared to Swedish drink tap water regulation which is probably because of our tap water samples from the pilot hall in the Chemical Engineering Department, Lund University is not designed for drinking (called processed water).
Further investigations should be given in the aspect of metal ions behavior in the tap water such as how the metal ions are combing to other materials and how the size distribution is related to their chemical-physical properties. (Less)
Popular Abstract
Nanoparticles (NPs) are day by day rise more concerned by human beings as micro pollutants have confirmed found in human stools. However, because of its extremely small size, relatively low concentrations and so many different sources of which some are hard to determine, qualifying and quantifying becoming extremely hard based on the technology we have now. Tap water in Skåne Sweden is directly drinkable, therefore, once the NPs remain in the water could directly enter human bodies and may cause an unwanted hazard. The toxicity induced by NPs are mainly by the heavy metals which have been detailed analyzed both of their kinds and concentrations.
The experiment we have done has found out that there exist NPs in tap water and the sizes are... (More)
Nanoparticles (NPs) are day by day rise more concerned by human beings as micro pollutants have confirmed found in human stools. However, because of its extremely small size, relatively low concentrations and so many different sources of which some are hard to determine, qualifying and quantifying becoming extremely hard based on the technology we have now. Tap water in Skåne Sweden is directly drinkable, therefore, once the NPs remain in the water could directly enter human bodies and may cause an unwanted hazard. The toxicity induced by NPs are mainly by the heavy metals which have been detailed analyzed both of their kinds and concentrations.
The experiment we have done has found out that there exist NPs in tap water and the sizes are widely distributed among the whole nano size range. The mean diameter of the NPs in tap water is 177.6 nm and most of them are around 100 to 300 nm which is quite concerned because those have less than 100 nm NPs could easily penetrate cell membranes and cause damages to all the cells including organs and brain.
The metals found in tap water has a lower concentration than the limit settled by the Swedish drink water regulation except for zinc and lead which both of them could probably release from the old piping system both in the distribution system or from the building. While some of the common metal ions are not detectable by our experiment settings. These need more experiments to have further investigation.
NPs in tap water (drinkable water) should have more attention as its infinite relation in human being’s daily life, and what we have done is the first step. (Less)
Please use this url to cite or link to this publication:
author
Zhang, Lingping LU
supervisor
organization
course
KLGM01 20191
year
type
H2 - Master's Degree (Two Years)
subject
keywords
tap water, nanoparticles, size distribution, metal ions, asymmetrical flow field-flow fractionation (AF4), food technology, livsmedelsteknologi
language
English
id
8985275
date added to LUP
2019-09-03 15:56:46
date last changed
2019-09-03 15:56:46
@misc{8985275,
  abstract     = {40 L tap water was ultra-filtered by M10 lab unit using 10 kDa regenerated membrane and 800 nm syringe filter to get the NPs. The filter NPs are then separated and characterized using asymmetrical flow field-flow fractionation with coupled multi angle light scattering (AF4-MALS). Calibration curve for determining the size distribution is made by polystyrene latex bead (20, 60, 100, 300, and 500 nm) to calculate the size distribution of the NPs in the tap water samples with the size range between 20 to 800 nm. The mean diameter of the NPs in tap water is 177.6 nm and most of them are around 100 to 300 nm. The different kinds of metal ions were analyzed for three different size fraction ranges (20 to 100 nm, 100 to 200 nm and 200 to 300 nm) by inductively coupled plasma mass spectroscopy (ICP-MS) and optical emission spectrometry (ICP-OES) techniques. As a result, Fe, Cu, Ni, and Ag were not detectable and Ca, Si, Al, Pb and Au have the high concentration in the 20 to 100 nm size range which has the highest possibility of toxicity. Zn and Pb are over the limit compared to Swedish drink tap water regulation which is probably because of our tap water samples from the pilot hall in the Chemical Engineering Department, Lund University is not designed for drinking (called processed water).
Further investigations should be given in the aspect of metal ions behavior in the tap water such as how the metal ions are combing to other materials and how the size distribution is related to their chemical-physical properties.},
  author       = {Zhang, Lingping},
  keyword      = {tap water,nanoparticles,size distribution,metal ions,asymmetrical flow field-flow fractionation (AF4),food technology,livsmedelsteknologi},
  language     = {eng},
  note         = {Student Paper},
  title        = {Develop a method to detect nanoparticles in tap water using AF4},
  year         = {2019},
}