How to optimize the extraction of active substance from polymeric materials
(2021) KMBM05 20211Applied Microbiology
- Abstract
- The aim of this thesis was to evaluate influencing parameters of extraction through polymers and find effective extraction methods. A second purpose was to compare differences and similarities of various polymers consisting of Ethylene Vinyl Acetate (EVA) when extracted with identical parameters. Vaginal rings made of EVA which were loaded with active substance were used for the study. The rings are used as a local drug delivery system for women treating uterine fibroids and endometriosis. Effective extraction methods for the rings are important with the purpose to ensure the rings are loaded with correct amount of active substance. The products must contain the desired amount of drug in order to work efficiently for the patient and treat... (More)
- The aim of this thesis was to evaluate influencing parameters of extraction through polymers and find effective extraction methods. A second purpose was to compare differences and similarities of various polymers consisting of Ethylene Vinyl Acetate (EVA) when extracted with identical parameters. Vaginal rings made of EVA which were loaded with active substance were used for the study. The rings are used as a local drug delivery system for women treating uterine fibroids and endometriosis. Effective extraction methods for the rings are important with the purpose to ensure the rings are loaded with correct amount of active substance. The products must contain the desired amount of drug in order to work efficiently for the patient and treat diseases without the risk of being an inefficient drug.
Active substance is capsuled in the core of a fiber with a cover of a polymer film of various skin thicknesses of different EVA-material. Different types of EVA were also used in the core, and a variety of extraction methods were applied to extract the active substance. The factors analyzed for this thesis was different EVA-material, skin thicknesses, temperature, shaking speed, time, preparation of fiber i.e., slicing and cutting. After the extraction with varied parameters the extracted amounts were identified and quantified with High Pressure Liquid Chromatography (HPLC).
Results indicate that increased temperature and load percentage of API in the core give rise to higher recoveries of extraction, while increased thickness of polymer film causes a decreased diffusion flux and lowers recoveries. Fibers extracted in different shaking rates resulted in equal recoveries, therefore it was concluded that the shaking rates did not affect the extraction. Parameters as slicing the fiber results in higher recoveries compared to using the fiber whole or cutting the fiber. In a general comparison between EVA33 and EVA40, it is concluded that the skin thickness of the film covering the ring have a greater impact on the recoveries compared to the different types of EVA-material used in the film. Another important parameter is the extraction medium as solvent, drug and polymer must have similar polarities for an effective extraction. (Less) - Popular Abstract (Swedish)
- Extraktion är en separationsteknik som används för att avskilja önskade ämnen från en blandning. Syftet är att utvärdera hur man kan påverka extraktionen av läkemedel genom vaginala ringar. De vaginala ringarna som användes till studien är tillverkade av materialet Etylenvinylacetat (EVA) blandat med läkemedel. Därefter har ringarna blivit inkapslade av filmer som endast består av EVA-material.
Materialets struktur ändras beroende på hur tätt packade molekylerna är. Materialet blir mer packat med molekyler när mängden av ämnet vinylacetat minskar i EVA-materialen, vilket medför svårare passering av läkemedlet genom ringarna. Två olika sorters EVA-material användes, EVA33 och EVA40. EVA33 har 33% av vinylacetat och EVA40 har 40%... (More) - Extraktion är en separationsteknik som används för att avskilja önskade ämnen från en blandning. Syftet är att utvärdera hur man kan påverka extraktionen av läkemedel genom vaginala ringar. De vaginala ringarna som användes till studien är tillverkade av materialet Etylenvinylacetat (EVA) blandat med läkemedel. Därefter har ringarna blivit inkapslade av filmer som endast består av EVA-material.
Materialets struktur ändras beroende på hur tätt packade molekylerna är. Materialet blir mer packat med molekyler när mängden av ämnet vinylacetat minskar i EVA-materialen, vilket medför svårare passering av läkemedlet genom ringarna. Två olika sorters EVA-material användes, EVA33 och EVA40. EVA33 har 33% av vinylacetat och EVA40 har 40% vinylacetat, som innebär att EVA33 är svårare att passera genom i jämförelse med EVA40.
Ringarna klipptes i jämnlånga cylindrar, så kallade fibrer. En del fibrer klipptes i mindre bitar och en del blev klippta i mitten längs med den avlånga sidan av fibern. Därefter exponerades fibrerna för ändringar såsom lösningsmedel, temperatur, skakhastighet, tid och beredning av fiber. Varje enskild fiber tillsattes i separata flaskor med lösningsmedel. Lösningen samlades efter extraktionen och analyserades för att fastställa mängden av det önskade ämnet i lösningen.
Resultaten från försöken visade att tjockleken av filmen runt fibern påverkade passagen av läkemedel på grund av längre väg som passeras innan läkemedlet når lösningsmedlet. Ökad temperatur och tid gav bättre resultat, men ökad skakhastighet hade ingen effekt på extraktionen. Aceton var effektivare som lösningsmedel än etanol och metanol. Resultaten visade även att fibrerna som blev klippta i mitten längs med den avlånga sidan av fibern gav bättre resultat i jämförelse med klippta i mindre bitar och hela fibrer. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9058282
- author
- Puro, Dilek LU
- supervisor
- organization
- course
- KMBM05 20211
- year
- 2021
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- Ethylene vinyl acetate, Extraction, HPLC, Applied microbiology
- language
- English
- id
- 9058282
- date added to LUP
- 2021-06-28 13:40:46
- date last changed
- 2021-07-19 12:14:11
@misc{9058282,
abstract = {{The aim of this thesis was to evaluate influencing parameters of extraction through polymers and find effective extraction methods. A second purpose was to compare differences and similarities of various polymers consisting of Ethylene Vinyl Acetate (EVA) when extracted with identical parameters. Vaginal rings made of EVA which were loaded with active substance were used for the study. The rings are used as a local drug delivery system for women treating uterine fibroids and endometriosis. Effective extraction methods for the rings are important with the purpose to ensure the rings are loaded with correct amount of active substance. The products must contain the desired amount of drug in order to work efficiently for the patient and treat diseases without the risk of being an inefficient drug.
Active substance is capsuled in the core of a fiber with a cover of a polymer film of various skin thicknesses of different EVA-material. Different types of EVA were also used in the core, and a variety of extraction methods were applied to extract the active substance. The factors analyzed for this thesis was different EVA-material, skin thicknesses, temperature, shaking speed, time, preparation of fiber i.e., slicing and cutting. After the extraction with varied parameters the extracted amounts were identified and quantified with High Pressure Liquid Chromatography (HPLC).
Results indicate that increased temperature and load percentage of API in the core give rise to higher recoveries of extraction, while increased thickness of polymer film causes a decreased diffusion flux and lowers recoveries. Fibers extracted in different shaking rates resulted in equal recoveries, therefore it was concluded that the shaking rates did not affect the extraction. Parameters as slicing the fiber results in higher recoveries compared to using the fiber whole or cutting the fiber. In a general comparison between EVA33 and EVA40, it is concluded that the skin thickness of the film covering the ring have a greater impact on the recoveries compared to the different types of EVA-material used in the film. Another important parameter is the extraction medium as solvent, drug and polymer must have similar polarities for an effective extraction.}},
author = {{Puro, Dilek}},
language = {{eng}},
note = {{Student Paper}},
title = {{How to optimize the extraction of active substance from polymeric materials}},
year = {{2021}},
}