A study of nanoparticles interaction with plasma membrane proteins.
(2022) KEMK10 20221Department of Chemistry
- Abstract (Swedish)
- Nanoparticles have gained considerable attention in the last decades, especially in the medical field. The nanoparticles could enter our bodies from anywhere in the surrounding environment and interact with objects in our biological fluids. However, there is little knowledge about this process. The protein competes for the nanoparticle's surface area, forming “corona” made of different proteins that largely defines the particle's biological identity. The composition of the biocorona plays an important role in nanoparticle biocompatibility. This interaction process depends on many factors, such as the size of the nanoparticle, the material of the nanoparticle, surface charge and the protein identity. In this work, we examined the... (More)
- Nanoparticles have gained considerable attention in the last decades, especially in the medical field. The nanoparticles could enter our bodies from anywhere in the surrounding environment and interact with objects in our biological fluids. However, there is little knowledge about this process. The protein competes for the nanoparticle's surface area, forming “corona” made of different proteins that largely defines the particle's biological identity. The composition of the biocorona plays an important role in nanoparticle biocompatibility. This interaction process depends on many factors, such as the size of the nanoparticle, the material of the nanoparticle, surface charge and the protein identity. In this work, we examined the composition of the protein corona formed from the interaction of the nanoparticles with the plasma membrane protein extracted from a mouse cell line. We show that the smaller sizes of carboxylated nanoparticles (PS-COOH) bind to higher amounts of proteins than the larger particles while the larger particles of aminated nanoparticles (PS-NH2) bind to more proteins than the smaller particles. This work also illustrates that the aminated nanoparticles (PS-NH2) are more toxic to cells than the carboxylated nanoparticles (PS-COOH) by using cytotoxicity and MTT cell proliferation studies. (Less)
- Popular Abstract
- Nanoparticles are small particles that the human eye cannot detect. They are particles of matter that are found in the natural world and created as a consequence of human activity. For a particle to be classified as a nanoparticle, it should have a diameter between 1 and 100 nm, larger particles up to 500 nm could be also classified as nanoparticles. Their submicroscopic size allows them to have distinct properties and unique applications. They could also be created or synthesized as a by-product from several chemical reactions to fulfil a specific purpose or specific function. The manufactured particles that are produced in a specific way to play a specific role have a variety of applications in medicine, cosmetic and environmental... (More)
- Nanoparticles are small particles that the human eye cannot detect. They are particles of matter that are found in the natural world and created as a consequence of human activity. For a particle to be classified as a nanoparticle, it should have a diameter between 1 and 100 nm, larger particles up to 500 nm could be also classified as nanoparticles. Their submicroscopic size allows them to have distinct properties and unique applications. They could also be created or synthesized as a by-product from several chemical reactions to fulfil a specific purpose or specific function. The manufactured particles that are produced in a specific way to play a specific role have a variety of applications in medicine, cosmetic and environmental remediation. For example, in the medicinal field, nanoparticles (NPs) have several important roles in the process of target-specific drug delivery.
The nanoparticles are classified into a variety of types according to their properties such as size, shape, and material. Examples of their classifications are, metal NPs, gold NPs and polystyrene NPs. These classifications are also divided up according to their material, for instance, the polystyrene NPs are also divided up into carbon-based nanoparticles (PS-COOH) and amine-based nanoparticles (PS-NH2). These polystyrene nanoparticles can be found in plastics and could penetrate our anatomy by different routes, such as skin, respiratory and food intake. The interactions of these particles with our cells are one of the research fields that is being studied extensively in the last decades. When NPs enter our body, our biological macromolecules such as protein and lipids compete for the nanoparticle's surface, promoting the formation of “corona” made of different proteins. The protein corona will have different protein compositions depending on the size of the nanoparticle and surface chemistry. The composition of the biocorona determines the biological identity of the particle, the cellular uptake of NPs and the way the particle is recognized by the immune system. The biocorona formed indicates the cell's interactions with the surrounding environment. Understanding the composition of the biocorona will help with improving research in the medicinal field, where the nanoparticles are used to deliver substances to specific cells or to enhance the immune response to weak antigens. In this study, the interaction between the plasma membrane proteins extracted from the mouse cell lines “RAW267.4” and the polystyrene carboxylated nanoparticles (PS-COOH) and aminated (PS-NH2) was examined. The proteins were extracted and incubated with these nanoparticles in different sizes to examine the effect of the size on the formation of the protein corona. Additionally, the toxicity of these particles on “RAW 267.4) cells was investigated to determine the level of damage that NPs induce to cells. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9086241
- author
- Snowbar, Abrar LU
- supervisor
-
- Tommy Cedervall LU
- Tania Lima LU
- organization
- course
- KEMK10 20221
- year
- 2022
- type
- M2 - Bachelor Degree
- subject
- keywords
- Nanoparticles, Plasma membrane protein, Carboxylated nanoparticles, Aminated nanoparticles, Biochemistry
- language
- English
- id
- 9086241
- date added to LUP
- 2022-09-06 12:44:47
- date last changed
- 2022-09-06 12:44:47
@misc{9086241,
abstract = {{Nanoparticles have gained considerable attention in the last decades, especially in the medical field. The nanoparticles could enter our bodies from anywhere in the surrounding environment and interact with objects in our biological fluids. However, there is little knowledge about this process. The protein competes for the nanoparticle's surface area, forming “corona” made of different proteins that largely defines the particle's biological identity. The composition of the biocorona plays an important role in nanoparticle biocompatibility. This interaction process depends on many factors, such as the size of the nanoparticle, the material of the nanoparticle, surface charge and the protein identity. In this work, we examined the composition of the protein corona formed from the interaction of the nanoparticles with the plasma membrane protein extracted from a mouse cell line. We show that the smaller sizes of carboxylated nanoparticles (PS-COOH) bind to higher amounts of proteins than the larger particles while the larger particles of aminated nanoparticles (PS-NH2) bind to more proteins than the smaller particles. This work also illustrates that the aminated nanoparticles (PS-NH2) are more toxic to cells than the carboxylated nanoparticles (PS-COOH) by using cytotoxicity and MTT cell proliferation studies.}},
author = {{Snowbar, Abrar}},
language = {{eng}},
note = {{Student Paper}},
title = {{A study of nanoparticles interaction with plasma membrane proteins.}},
year = {{2022}},
}