Microbial transformation of 5-hydroxymethylfurfural (HMF) to 2,5-Bis(hydroxymethyl)furan (BHMF).
(2024) KBTM01 20241Biotechnology (MSc)
Biotechnology (M.Sc.Eng.)
- Abstract
- The chemical industry is particularly resource-intensive, consuming a substantial portion of petroleum resources and significantly contributing to greenhouse gas emissions. Consequently, there is an urgent need to explore sustainable alternatives. Biomass has emerged as a promising renewable resource. The dehydration of biomass-derived carbohydrates has proven to be an effective method, producing key intermediates such as 5-hydroxymethylfurfural (HMF). HMF is a versatile platform chemical that paves the way for various valuable bioproducts, providing a sustainable solution to reduce reliance on fossil fuels and mitigate environmental impacts.
This study focuses on using Mycobacterium MS1601, a bacterium identified for its natural... (More) - The chemical industry is particularly resource-intensive, consuming a substantial portion of petroleum resources and significantly contributing to greenhouse gas emissions. Consequently, there is an urgent need to explore sustainable alternatives. Biomass has emerged as a promising renewable resource. The dehydration of biomass-derived carbohydrates has proven to be an effective method, producing key intermediates such as 5-hydroxymethylfurfural (HMF). HMF is a versatile platform chemical that paves the way for various valuable bioproducts, providing a sustainable solution to reduce reliance on fossil fuels and mitigate environmental impacts.
This study focuses on using Mycobacterium MS1601, a bacterium identified for its natural ability to reduce HMF to BHMF through whole-cell catalysis. This study focused on optimizing the bioconversion of 5-hydroxymethylfurfural (HMF) to 2,5-bis(hydroxymethyl)furan (BHMF) using Mycobacterium sp. MS1601. Key parameters such as incubation duration, carbon source, pH, cell concentration, substrate concentration, and anaerobic conditions were evaluated. Glucose emerged as the optimal carbon source, achieving a 63.2% BHMF yield at pH 7 within 24 hours. A cell concentration of 50 gCDW/L significantly improved BHMF yield to 80.8%, while NADH supplementation did not enhance production. Lower substrate concentrations were found to be optimal, and aerobic conditions favored higher yields. Upscale bioreactor experiments demonstrated significant improvements in BHMF production, achieving a rapid increase from 9.8% to 63.0% over 24 hours. BHMF was successfully purified using liquid-liquid extraction followed by flash chromatography. Additionally, a potential dehydrogenase gene responsible for HMF reduction was identified, amplified, and cloned, providing a basis for future genetic optimization and industrial applications
These findings highlight the potential of Mycobacterium sp. MS1601 as a robust biocatalyst for sustainable BHMF production. The study's optimized conditions and gene identification provide a foundation for future genetic enhancements and industrial applications. This research contributes to the development of environmentally friendly processes, offering a viable alternative to fossil-based chemicals and paving the way for greener industrial practices. (Less) - Popular Abstract
- Our world faces an urgent challenge: reducing our reliance on fossil fuels, which contribute heavily to pollution, climate change, and resource depletion. One promising solution lies in harnessing renewable resources to create biobased materials – materials derived from plants rather than petroleum. In this study, we explored how a bacterium called Mycobacterium sp. MS1601 a remarkable wild-type bacterium, that can convert 5-hydroxymethylfurfural (HMF), a plant-based compound, into 2,5-bis(hydroxymethyl)furan (BHMF) — a valuable building block for sustainable materials. This biobased compound has the potential to revolutionize industries and help create a cleaner, greener future.
BHMF can be used to produce biobased polymers for... (More) - Our world faces an urgent challenge: reducing our reliance on fossil fuels, which contribute heavily to pollution, climate change, and resource depletion. One promising solution lies in harnessing renewable resources to create biobased materials – materials derived from plants rather than petroleum. In this study, we explored how a bacterium called Mycobacterium sp. MS1601 a remarkable wild-type bacterium, that can convert 5-hydroxymethylfurfural (HMF), a plant-based compound, into 2,5-bis(hydroxymethyl)furan (BHMF) — a valuable building block for sustainable materials. This biobased compound has the potential to revolutionize industries and help create a cleaner, greener future.
BHMF can be used to produce biobased polymers for packaging, textiles, and automotive parts, helping to reduce plastic pollution and lower carbon emissions. BHMF-derived biodiesel additives improve fuel efficiency and reduce vehicle emissions, supporting cleaner transportation. Additionally, BHMF can be converted into eco-friendly surfactants for detergents and personal care products, which are safer for aquatic life. It also serves as a non-toxic alternative for plasticizers and flame retardants, replacing harmful chemicals. By utilizing BHMF-based materials, we reduce fossil fuel dependence, pollution, and health risks, promoting a more sustainable future.
In this research, we aimed to optimize the conditions for Mycobacterium sp. MS1601 to efficiently convert HMF into BHMF. This study achieved an 80% HMF to BHMF bioconversion within just 4 hours under ideal conditions. Additionally, we identified and amplified a potential dehydrogenase gene responsible for this conversion, laying the groundwork for future genetic enhancements and industrial applications.
By harnessing the power of Mycobacterium sp. MS1601, an unmodified wild-type bacterium, this study unlocks a natural ally in the quest for a greener future. The ability of this resilient microbe to convert HMF into BHMF and other valuable derivatives offers a tangible solution to reducing our reliance on fossil fuels. These biobased materials have the potential to touch our daily lives — from safer cleaning products to cleaner fuels and healthier, non-toxic alternatives in flexible plastics. By choosing nature's pathways, we take a step toward a healthier planet and a future where innovation works in harmony with the environment. (Less)
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/student-papers/record/9185424
- author
- Hamza, Faqiha Ali LU
- supervisor
- organization
- course
- KBTM01 20241
- year
- 2024
- type
- H2 - Master's Degree (Two Years)
- subject
- keywords
- Biotechnology, Microbiology, Biotransformation
- language
- English
- id
- 9185424
- date added to LUP
- 2025-02-19 14:50:14
- date last changed
- 2025-02-19 14:50:14
@misc{9185424,
abstract = {{The chemical industry is particularly resource-intensive, consuming a substantial portion of petroleum resources and significantly contributing to greenhouse gas emissions. Consequently, there is an urgent need to explore sustainable alternatives. Biomass has emerged as a promising renewable resource. The dehydration of biomass-derived carbohydrates has proven to be an effective method, producing key intermediates such as 5-hydroxymethylfurfural (HMF). HMF is a versatile platform chemical that paves the way for various valuable bioproducts, providing a sustainable solution to reduce reliance on fossil fuels and mitigate environmental impacts.
This study focuses on using Mycobacterium MS1601, a bacterium identified for its natural ability to reduce HMF to BHMF through whole-cell catalysis. This study focused on optimizing the bioconversion of 5-hydroxymethylfurfural (HMF) to 2,5-bis(hydroxymethyl)furan (BHMF) using Mycobacterium sp. MS1601. Key parameters such as incubation duration, carbon source, pH, cell concentration, substrate concentration, and anaerobic conditions were evaluated. Glucose emerged as the optimal carbon source, achieving a 63.2% BHMF yield at pH 7 within 24 hours. A cell concentration of 50 gCDW/L significantly improved BHMF yield to 80.8%, while NADH supplementation did not enhance production. Lower substrate concentrations were found to be optimal, and aerobic conditions favored higher yields. Upscale bioreactor experiments demonstrated significant improvements in BHMF production, achieving a rapid increase from 9.8% to 63.0% over 24 hours. BHMF was successfully purified using liquid-liquid extraction followed by flash chromatography. Additionally, a potential dehydrogenase gene responsible for HMF reduction was identified, amplified, and cloned, providing a basis for future genetic optimization and industrial applications
These findings highlight the potential of Mycobacterium sp. MS1601 as a robust biocatalyst for sustainable BHMF production. The study's optimized conditions and gene identification provide a foundation for future genetic enhancements and industrial applications. This research contributes to the development of environmentally friendly processes, offering a viable alternative to fossil-based chemicals and paving the way for greener industrial practices.}},
author = {{Hamza, Faqiha Ali}},
language = {{eng}},
note = {{Student Paper}},
title = {{Microbial transformation of 5-hydroxymethylfurfural (HMF) to 2,5-Bis(hydroxymethyl)furan (BHMF).}},
year = {{2024}},
}