Human monoclonal antibodies against an epitope on the class 5c outer membrane protein common to many pathogenic strains of Neisseria meningitidis
(1992) In Journal of Infectious Diseases 166(6). p.1322-1328- Abstract
Neisseria meningitidis is a causative agent of meningitis. Despite vaccination programs, it still causes a large number of deaths in young children. Early diagnosis followed by passive immunization with human monoclonal antibodies could be an approach to effective therapy. Peripheral blood lymphocytes from normal, healthy blood donors and from vaccinated individuals were immunized in vitro, using outer membrane proteins purified from A. meningitidis B:4:P1,15. The immunized human B cells were Epstein-Barr virus transformed and fused to a heteromyeloma. Several stable human hybridoma cell lines were established and two, secreting antibodies against the 31-kDa class 5c outer membrane protein, were characterized further. The human... (More)
Neisseria meningitidis is a causative agent of meningitis. Despite vaccination programs, it still causes a large number of deaths in young children. Early diagnosis followed by passive immunization with human monoclonal antibodies could be an approach to effective therapy. Peripheral blood lymphocytes from normal, healthy blood donors and from vaccinated individuals were immunized in vitro, using outer membrane proteins purified from A. meningitidis B:4:P1,15. The immunized human B cells were Epstein-Barr virus transformed and fused to a heteromyeloma. Several stable human hybridoma cell lines were established and two, secreting antibodies against the 31-kDa class 5c outer membrane protein, were characterized further. The human antibodies were of IgG1 and IgG3 isotypes, with κ light chains. The recognized epitope was commonly found among pathogenic strains of N. meningitidis; thus, these human monoclonal antibodies may be important in the evaluation of N. meningitidis infections.
(Less)
- author
- De Cossío, Maria E Fernández ; Ohlin, Mats LU ; Llano, Manual ; Selander, Barbro LU ; Cruz, Silian ; Del Valle, Jesus and Borrebaeck, Carl A K LU
- organization
- publishing date
- 1992
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Infectious Diseases
- volume
- 166
- issue
- 6
- pages
- 7 pages
- publisher
- Oxford University Press
- external identifiers
-
- scopus:0026489196
- pmid:1385548
- ISSN
- 0022-1899
- DOI
- 10.1093/infdis/166.6.1322
- language
- English
- LU publication?
- yes
- id
- 052dfd88-b119-4c95-90e6-dae06dc82a4e
- date added to LUP
- 2016-04-20 16:11:50
- date last changed
- 2024-01-04 02:31:56
@article{052dfd88-b119-4c95-90e6-dae06dc82a4e, abstract = {{<p>Neisseria meningitidis is a causative agent of meningitis. Despite vaccination programs, it still causes a large number of deaths in young children. Early diagnosis followed by passive immunization with human monoclonal antibodies could be an approach to effective therapy. Peripheral blood lymphocytes from normal, healthy blood donors and from vaccinated individuals were immunized in vitro, using outer membrane proteins purified from A. meningitidis B:4:P1,15. The immunized human B cells were Epstein-Barr virus transformed and fused to a heteromyeloma. Several stable human hybridoma cell lines were established and two, secreting antibodies against the 31-kDa class 5c outer membrane protein, were characterized further. The human antibodies were of IgG1 and IgG3 isotypes, with κ light chains. The recognized epitope was commonly found among pathogenic strains of N. meningitidis; thus, these human monoclonal antibodies may be important in the evaluation of N. meningitidis infections.</p>}}, author = {{De Cossío, Maria E Fernández and Ohlin, Mats and Llano, Manual and Selander, Barbro and Cruz, Silian and Del Valle, Jesus and Borrebaeck, Carl A K}}, issn = {{0022-1899}}, language = {{eng}}, number = {{6}}, pages = {{1322--1328}}, publisher = {{Oxford University Press}}, series = {{Journal of Infectious Diseases}}, title = {{Human monoclonal antibodies against an epitope on the class 5c outer membrane protein common to many pathogenic strains of Neisseria meningitidis}}, url = {{http://dx.doi.org/10.1093/infdis/166.6.1322}}, doi = {{10.1093/infdis/166.6.1322}}, volume = {{166}}, year = {{1992}}, }