Identifying bacteria using DNA binding maps
(2013) 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 1. p.473-475- Abstract
We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces... (More)
We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.
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- author
- Emilsson, Gustav ; Nilsson, Adam LU ; Nyberg, Lena K. LU ; Noble, Charleston LU ; Svensson Stadler, Liselott ; Moore, Edward R.B. ; Ambjörnsson, Tobias LU ; Tegenfeldt, Jonas O. LU and Westerlund, Fredrik
- organization
- publishing date
- 2013-01-01
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Bacterial identification, Nanofluidics, Optical mapping
- host publication
- 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
- volume
- 1
- pages
- 3 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
- conference location
- Freiburg, Germany
- conference dates
- 2013-10-27 - 2013-10-31
- external identifiers
-
- scopus:84907335679
- ISBN
- 9781632666246
- language
- English
- LU publication?
- yes
- id
- 05f18cd8-f5c0-45d0-bc9e-83a96d76a15f
- date added to LUP
- 2018-10-20 08:40:25
- date last changed
- 2024-01-15 04:36:52
@inproceedings{05f18cd8-f5c0-45d0-bc9e-83a96d76a15f, abstract = {{<p>We have developed an assay, based on nanofluidic channels and fluorescence microscopy, for optical mapping of DNA based on competitive binding between two molecules - one fluorescent and one sequence selective. From the experimental data we can extract binding constants for the two competing DNA binders, which may be subsequently used to calculate a theoretical reference map of any DNA with known sequence. The goal is to create a method for fast identification of bacteria from single DNA molecules without the need for additional cultivation or amplification. We here demonstrate a proof-of-principle experiment on phage DNA and furthermore show that the method can be used to distinguish between two strains of E. coli DNA and to map pieces of DNA onto the full genome.</p>}}, author = {{Emilsson, Gustav and Nilsson, Adam and Nyberg, Lena K. and Noble, Charleston and Svensson Stadler, Liselott and Moore, Edward R.B. and Ambjörnsson, Tobias and Tegenfeldt, Jonas O. and Westerlund, Fredrik}}, booktitle = {{17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013}}, isbn = {{9781632666246}}, keywords = {{Bacterial identification; Nanofluidics; Optical mapping}}, language = {{eng}}, month = {{01}}, pages = {{473--475}}, publisher = {{Chemical and Biological Microsystems Society}}, title = {{Identifying bacteria using DNA binding maps}}, volume = {{1}}, year = {{2013}}, }