Carrier detection in hemophilia A : a cooperative international study. II. The efficacy of a universal discriminant
(1986) In Blood 67(6). p.7-1560- Abstract
Factor VIII (F.VIII) and von Willebrand factor (VWF):Ag data collected by eight laboratories on a total of 336 obligatory carriers of hemophilia A and 137 normal women were used to answer several questions concerning the construction of linear discriminants for carrier detection. It was found: that a "universal" linear discriminant can be constructed which is suitable for use in all laboratories and is nearly as effective as laboratory-specific discriminants; that inclusion of age and ABO blood type data improved the efficacy of these discriminants; that substitution of alternative assays for F.VIII and VWF:Ag did not generally improve the efficacy of the discriminants over that obtained using the bioassay for F.VIII:C and Laurell's... (More)
Factor VIII (F.VIII) and von Willebrand factor (VWF):Ag data collected by eight laboratories on a total of 336 obligatory carriers of hemophilia A and 137 normal women were used to answer several questions concerning the construction of linear discriminants for carrier detection. It was found: that a "universal" linear discriminant can be constructed which is suitable for use in all laboratories and is nearly as effective as laboratory-specific discriminants; that inclusion of age and ABO blood type data improved the efficacy of these discriminants; that substitution of alternative assays for F.VIII and VWF:Ag did not generally improve the efficacy of the discriminants over that obtained using the bioassay for F.VIII:C and Laurell's immunoassay for VWF:Ag; that linear discriminants were far more effective than discriminants based on the F.VIII:C/VWF:Ag ratio. A step-wise procedure is given which any laboratory may follow in using the universal discriminant for carrier detection.
(Less)
- author
- Green, P P ; Mannucci, P M ; Briët, E ; Ljung, R LU ; Kasper, C K ; Essien, E M ; Chediak, J ; Rizza, C R and Graham, J B
- organization
- publishing date
- 1986-06
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- ABO Blood-Group System, Adult, Antigens, Enzyme-Linked Immunosorbent Assay, Factor VIII, Female, Hemophilia A, Heterozygote Detection, Humans, Immunoenzyme Techniques, International Cooperation, Phenotype, von Willebrand Factor, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.
- in
- Blood
- volume
- 67
- issue
- 6
- pages
- 7 - 1560
- publisher
- American Society of Hematology
- external identifiers
-
- scopus:0022453895
- pmid:3085744
- ISSN
- 0006-4971
- language
- English
- LU publication?
- yes
- id
- 05fba024-738c-432f-9247-ee00cb4edc69
- date added to LUP
- 2016-10-26 14:01:21
- date last changed
- 2024-01-19 11:21:53
@article{05fba024-738c-432f-9247-ee00cb4edc69, abstract = {{<p>Factor VIII (F.VIII) and von Willebrand factor (VWF):Ag data collected by eight laboratories on a total of 336 obligatory carriers of hemophilia A and 137 normal women were used to answer several questions concerning the construction of linear discriminants for carrier detection. It was found: that a "universal" linear discriminant can be constructed which is suitable for use in all laboratories and is nearly as effective as laboratory-specific discriminants; that inclusion of age and ABO blood type data improved the efficacy of these discriminants; that substitution of alternative assays for F.VIII and VWF:Ag did not generally improve the efficacy of the discriminants over that obtained using the bioassay for F.VIII:C and Laurell's immunoassay for VWF:Ag; that linear discriminants were far more effective than discriminants based on the F.VIII:C/VWF:Ag ratio. A step-wise procedure is given which any laboratory may follow in using the universal discriminant for carrier detection.</p>}}, author = {{Green, P P and Mannucci, P M and Briët, E and Ljung, R and Kasper, C K and Essien, E M and Chediak, J and Rizza, C R and Graham, J B}}, issn = {{0006-4971}}, keywords = {{ABO Blood-Group System; Adult; Antigens; Enzyme-Linked Immunosorbent Assay; Factor VIII; Female; Hemophilia A; Heterozygote Detection; Humans; Immunoenzyme Techniques; International Cooperation; Phenotype; von Willebrand Factor; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.}}, language = {{eng}}, number = {{6}}, pages = {{7--1560}}, publisher = {{American Society of Hematology}}, series = {{Blood}}, title = {{Carrier detection in hemophilia A : a cooperative international study. II. The efficacy of a universal discriminant}}, volume = {{67}}, year = {{1986}}, }