Recovery of recombinant cutinase using detergent foam
(2002) In Biotechnology Progress 18(1). p.116-123- Abstract
- Foam generated by vigorous stirring of a nonionic detergent, Triton X-114, was used for the recovery of recombinant cutinase expressed by Saccharomyces cerevisiae. The enzyme with a hydrophobic fusion tag, (Trp-Pro)(4), was recovered with a higher yield as compared to the wild-type cutinase, indicating the involvement of hydrophobic interactions in protein isolation with the foam. The influence of various factors including volume, dilution, pH, different additives, and cell concentration in the medium on enzyme recovery was investigated. Interaction of the enzyme with detergent was monitored using fluorescence spectroscopy. No significant changes in protein conformation after the isolation procedure were observed using circular dichroism.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106246
- author
- Fernandes, Sheryl ; Mattiasson, Bo LU and Hatti-Kaul, Rajni LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biotechnology Progress
- volume
- 18
- issue
- 1
- pages
- 116 - 123
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000173819100017
- scopus:0036008327
- ISSN
- 1520-6033
- DOI
- 10.1021/bp010161m
- language
- English
- LU publication?
- yes
- id
- 702388dc-d9c3-4ad2-a98e-2cc646b15713 (old id 106246)
- date added to LUP
- 2016-04-01 16:41:24
- date last changed
- 2022-02-27 22:55:39
@article{702388dc-d9c3-4ad2-a98e-2cc646b15713, abstract = {{Foam generated by vigorous stirring of a nonionic detergent, Triton X-114, was used for the recovery of recombinant cutinase expressed by Saccharomyces cerevisiae. The enzyme with a hydrophobic fusion tag, (Trp-Pro)(4), was recovered with a higher yield as compared to the wild-type cutinase, indicating the involvement of hydrophobic interactions in protein isolation with the foam. The influence of various factors including volume, dilution, pH, different additives, and cell concentration in the medium on enzyme recovery was investigated. Interaction of the enzyme with detergent was monitored using fluorescence spectroscopy. No significant changes in protein conformation after the isolation procedure were observed using circular dichroism.}}, author = {{Fernandes, Sheryl and Mattiasson, Bo and Hatti-Kaul, Rajni}}, issn = {{1520-6033}}, language = {{eng}}, number = {{1}}, pages = {{116--123}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Biotechnology Progress}}, title = {{Recovery of recombinant cutinase using detergent foam}}, url = {{http://dx.doi.org/10.1021/bp010161m}}, doi = {{10.1021/bp010161m}}, volume = {{18}}, year = {{2002}}, }