A divalent antibody format is required for neutralization of human cytomegalovirus via antigenic domain 2 on glycoprotein B
(2002) In Journal of General Virology 83(Pt 8). p.2001-2005- Abstract
- Glycoprotein B (gB) of human cytomegalovirus (HCMV) is the dominating protein in the envelope of this virus and gives rise to virus-neutralizing antibodies in most infected individuals. We have previously isolated a neutralizing human antibody specific for antigenic domain 2 (AD-2) on gB, a poorly immunogenic epitope, which nevertheless is capable of eliciting potent neutralizing antibodies. In order to define parameters important for the neutralization of HCMV via gB, we have investigated the virus-neutralizing capacity and the kinetics of the interaction with AD-2 of the monomeric and dimeric forms of a single chain variable fragment (scFv) corresponding to this antibody. We demonstrate here that neutralization of HCMV via AD-2 on gB can... (More)
- Glycoprotein B (gB) of human cytomegalovirus (HCMV) is the dominating protein in the envelope of this virus and gives rise to virus-neutralizing antibodies in most infected individuals. We have previously isolated a neutralizing human antibody specific for antigenic domain 2 (AD-2) on gB, a poorly immunogenic epitope, which nevertheless is capable of eliciting potent neutralizing antibodies. In order to define parameters important for the neutralization of HCMV via gB, we have investigated the virus-neutralizing capacity and the kinetics of the interaction with AD-2 of the monomeric and dimeric forms of a single chain variable fragment (scFv) corresponding to this antibody. We demonstrate here that neutralization of HCMV via AD-2 on gB can be mediated by dimeric scFv, while monomeric fragments cannot mediate neutralization of the virus, despite a slow dissociation from the intact glycoprotein. This finding is discussed in the context of possible mechanisms for antibody-mediated virus neutralization. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/109429
- author
- Lantto, Johan LU ; Fletcher, Jean M and Ohlin, Mats LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of General Virology
- volume
- 83
- issue
- Pt 8
- pages
- 2001 - 2005
- publisher
- Microbiology Society
- external identifiers
-
- pmid:12124464
- wos:000176989900019
- scopus:0036322438
- ISSN
- 1465-2099
- language
- English
- LU publication?
- yes
- id
- dd6176c6-8f80-467a-89c6-467693d42e6e (old id 109429)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12124464&dopt=Abstract
- date added to LUP
- 2016-04-01 15:25:53
- date last changed
- 2022-03-30 01:15:09
@article{dd6176c6-8f80-467a-89c6-467693d42e6e, abstract = {{Glycoprotein B (gB) of human cytomegalovirus (HCMV) is the dominating protein in the envelope of this virus and gives rise to virus-neutralizing antibodies in most infected individuals. We have previously isolated a neutralizing human antibody specific for antigenic domain 2 (AD-2) on gB, a poorly immunogenic epitope, which nevertheless is capable of eliciting potent neutralizing antibodies. In order to define parameters important for the neutralization of HCMV via gB, we have investigated the virus-neutralizing capacity and the kinetics of the interaction with AD-2 of the monomeric and dimeric forms of a single chain variable fragment (scFv) corresponding to this antibody. We demonstrate here that neutralization of HCMV via AD-2 on gB can be mediated by dimeric scFv, while monomeric fragments cannot mediate neutralization of the virus, despite a slow dissociation from the intact glycoprotein. This finding is discussed in the context of possible mechanisms for antibody-mediated virus neutralization.}}, author = {{Lantto, Johan and Fletcher, Jean M and Ohlin, Mats}}, issn = {{1465-2099}}, language = {{eng}}, number = {{Pt 8}}, pages = {{2001--2005}}, publisher = {{Microbiology Society}}, series = {{Journal of General Virology}}, title = {{A divalent antibody format is required for neutralization of human cytomegalovirus via antigenic domain 2 on glycoprotein B}}, url = {{https://lup.lub.lu.se/search/files/4391149/623636.pdf}}, volume = {{83}}, year = {{2002}}, }