Electroconvulsive seizures increase hippocampal neurogenesis after chronic corticosterone treatment.
(2002) In European Journal of Neuroscience 16(2). p.283-290- Abstract
- Major depression is often associated with elevated glucocorticoid levels. High levels of glucocorticoids reduce neurogenesis in the adult rat hippocampus. Electroconvulsive seizures (ECS) can enhance neurogenesis, and we investigated the effects of ECS in rats where glucocorticoid levels were elevated in order to mimic conditions seen in depression. Rats given injections of corticosterone or vehicle for 21 days were at the end of this period treated with either a single or five daily ECSs. Proliferating cells were labelled with bromodeoxyuridine (BrdU). After 3 weeks, BrdU-positive cells in the dentate gyrus were quantified and analyzed for co-labelling with the neuronal marker neuron-specific nuclear protein (NeuN). In... (More)
- Major depression is often associated with elevated glucocorticoid levels. High levels of glucocorticoids reduce neurogenesis in the adult rat hippocampus. Electroconvulsive seizures (ECS) can enhance neurogenesis, and we investigated the effects of ECS in rats where glucocorticoid levels were elevated in order to mimic conditions seen in depression. Rats given injections of corticosterone or vehicle for 21 days were at the end of this period treated with either a single or five daily ECSs. Proliferating cells were labelled with bromodeoxyuridine (BrdU). After 3 weeks, BrdU-positive cells in the dentate gyrus were quantified and analyzed for co-labelling with the neuronal marker neuron-specific nuclear protein (NeuN). In corticosterone-treated rats, neurogenesis was decreased by 75%. This was counteracted by a single ECS. Multiple ECS further increased neurogenesis and no significant differences in BrdU/NeuN positive cells were detected between corticosterone- and vehicle-treated rats given five ECS. Approximately 80% of the cells within the granule cell layer and 10% of the hilar cells were double-labelled with BrdU and NeuN. We therefore conclude that electroconvulsive seizures can increase hippocampal neurogenesis even in the presence of elevated levels of glucocorticoids. This further supports the hypothesis that induction of neurogenesis is an important event in the action of antidepressant treatment. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/109852
- author
- Hellsten, Johan LU ; Wennström, Malin LU ; Mohapel, Paul LU ; Ekdahl Clementson, Christine LU ; Bengzon, Johan LU and Tingström, Anders LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Neuroscience
- volume
- 16
- issue
- 2
- pages
- 283 - 290
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000177364000011
- pmid:12169110
- scopus:0036974526
- ISSN
- 1460-9568
- DOI
- 10.1046/j.1460-9568.2002.02093.x
- language
- English
- LU publication?
- yes
- id
- d270a588-7ff2-46b5-949a-4afae2f317c1 (old id 109852)
- alternative location
- http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=12169110&dopt=Abstract
- date added to LUP
- 2016-04-01 11:42:30
- date last changed
- 2022-02-03 03:23:38
@article{d270a588-7ff2-46b5-949a-4afae2f317c1,
abstract = {{Major depression is often associated with elevated glucocorticoid levels. High levels of glucocorticoids reduce neurogenesis in the adult rat hippocampus. Electroconvulsive seizures (ECS) can enhance neurogenesis, and we investigated the effects of ECS in rats where glucocorticoid levels were elevated in order to mimic conditions seen in depression. Rats given injections of corticosterone or vehicle for 21 days were at the end of this period treated with either a single or five daily ECSs. Proliferating cells were labelled with bromodeoxyuridine (BrdU). After 3 weeks, BrdU-positive cells in the dentate gyrus were quantified and analyzed for co-labelling with the neuronal marker neuron-specific nuclear protein (NeuN). In corticosterone-treated rats, neurogenesis was decreased by 75%. This was counteracted by a single ECS. Multiple ECS further increased neurogenesis and no significant differences in BrdU/NeuN positive cells were detected between corticosterone- and vehicle-treated rats given five ECS. Approximately 80% of the cells within the granule cell layer and 10% of the hilar cells were double-labelled with BrdU and NeuN. We therefore conclude that electroconvulsive seizures can increase hippocampal neurogenesis even in the presence of elevated levels of glucocorticoids. This further supports the hypothesis that induction of neurogenesis is an important event in the action of antidepressant treatment.}},
author = {{Hellsten, Johan and Wennström, Malin and Mohapel, Paul and Ekdahl Clementson, Christine and Bengzon, Johan and Tingström, Anders}},
issn = {{1460-9568}},
language = {{eng}},
number = {{2}},
pages = {{283--290}},
publisher = {{Wiley-Blackwell}},
series = {{European Journal of Neuroscience}},
title = {{Electroconvulsive seizures increase hippocampal neurogenesis after chronic corticosterone treatment.}},
url = {{https://lup.lub.lu.se/search/files/2605177/623642.pdf}},
doi = {{10.1046/j.1460-9568.2002.02093.x}},
volume = {{16}},
year = {{2002}},
}