False positive results in class-specific rheumatoid factor (RF) assays due to interaction between RF and Fc fragments of anti-immunoglobulin indicator reagents
(1983) In Journal of Immunological Methods 58(1-2). p.171-182- Abstract
- A recently developed, simple immunological method, diffusion-in-gel ELISA, has been adapted for class-specific determination of rheumatoid factor (RF). Evidence was obtained of an analytical error due to interaction between RF and antigenic determinants on the Fc part of the indicator immunoglobulin (Ig) molecules used. This was eliminated by replacing the usual indicator reagent, complete Ig molecules, by conjugated Fab or F(ab')2 fragments. The findings imply that unless the RF-Fc interaction mentioned is avoided in the assaying technique, false positive results may be obtained for, e.g. IgG RF and IgA RF in sera containing IgM RF.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1103008
- author
- Tarkowski, Andrej ; Bjursten, Lars Magnus LU ; Nilsson, Lars-Åke and Nygren, Håkan
- publishing date
- 1983
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- rheumatoid factor, immunoassay, immunoglobulin class
- in
- Journal of Immunological Methods
- volume
- 58
- issue
- 1-2
- pages
- 171 - 182
- publisher
- Elsevier
- external identifiers
-
- pmid:6601157
- scopus:0020672078
- ISSN
- 1872-7905
- DOI
- 10.1016/0022-1759(83)90273-9
- language
- English
- LU publication?
- no
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Bioimplant Research (013242910)
- id
- e24f121c-58c3-4fa9-bec5-73311b5f5f05 (old id 1103008)
- date added to LUP
- 2016-04-01 16:24:52
- date last changed
- 2021-01-03 06:07:59
@article{e24f121c-58c3-4fa9-bec5-73311b5f5f05, abstract = {{A recently developed, simple immunological method, diffusion-in-gel ELISA, has been adapted for class-specific determination of rheumatoid factor (RF). Evidence was obtained of an analytical error due to interaction between RF and antigenic determinants on the Fc part of the indicator immunoglobulin (Ig) molecules used. This was eliminated by replacing the usual indicator reagent, complete Ig molecules, by conjugated Fab or F(ab')2 fragments. The findings imply that unless the RF-Fc interaction mentioned is avoided in the assaying technique, false positive results may be obtained for, e.g. IgG RF and IgA RF in sera containing IgM RF.}}, author = {{Tarkowski, Andrej and Bjursten, Lars Magnus and Nilsson, Lars-Åke and Nygren, Håkan}}, issn = {{1872-7905}}, keywords = {{rheumatoid factor; immunoassay; immunoglobulin class}}, language = {{eng}}, number = {{1-2}}, pages = {{171--182}}, publisher = {{Elsevier}}, series = {{Journal of Immunological Methods}}, title = {{False positive results in class-specific rheumatoid factor (RF) assays due to interaction between RF and Fc fragments of anti-immunoglobulin indicator reagents}}, url = {{http://dx.doi.org/10.1016/0022-1759(83)90273-9}}, doi = {{10.1016/0022-1759(83)90273-9}}, volume = {{58}}, year = {{1983}}, }