Enhancement of hepatitis A propagation in tissue culture with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole
(1988) In Journal of Medical Virology 24(4). p.369-376- Abstract
- The adenosine analog 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) was found to increase the production of hepatitis A (HAV) antigen in two monkey kidney cell lines (Frhk-4 and Vero cells). DRB, a known inhibitor of the synthesis of messenger RNA, caused moderate changes in cell morphology. However, Frhk-4 cells could be maintained for several weeks at 80 microM of DRB, the concentration that caused maximal enhancement on HAV. DRB should be present from about the time of virus inoculation and its strongest effect was seen at low multiplicities of infection. Using radioimmunofocus assay it could be shown that DRB increased the amount of infectious virus. DRB treatment was applied in primary isolation of HAV from feces. In nine of... (More)
- The adenosine analog 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) was found to increase the production of hepatitis A (HAV) antigen in two monkey kidney cell lines (Frhk-4 and Vero cells). DRB, a known inhibitor of the synthesis of messenger RNA, caused moderate changes in cell morphology. However, Frhk-4 cells could be maintained for several weeks at 80 microM of DRB, the concentration that caused maximal enhancement on HAV. DRB should be present from about the time of virus inoculation and its strongest effect was seen at low multiplicities of infection. Using radioimmunofocus assay it could be shown that DRB increased the amount of infectious virus. DRB treatment was applied in primary isolation of HAV from feces. In nine of ten strains HAV antigen expression was strongly increased and in six of the ten strains infectivity of harvested material increased by one 10log or more. DRB thus seems to be a useful enhancer of HAV growth in tissue culture. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1104058
- author
- Widell, Anders LU ; Hansson, Bengt-Göran LU ; Nordenfelt, Erik and Öberg, Bo
- organization
- publishing date
- 1988
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- virus enhancement, hepatitis A virus, HAV in tissue culture
- in
- Journal of Medical Virology
- volume
- 24
- issue
- 4
- pages
- 369 - 376
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:2835431
- scopus:0023941497
- ISSN
- 1096-9071
- DOI
- 10.1002/jmv.1890240403
- language
- English
- LU publication?
- yes
- id
- 21944b41-7237-4272-ae8f-2f3a021b0151 (old id 1104058)
- date added to LUP
- 2016-04-01 11:42:13
- date last changed
- 2021-01-03 03:42:25
@article{21944b41-7237-4272-ae8f-2f3a021b0151, abstract = {{The adenosine analog 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) was found to increase the production of hepatitis A (HAV) antigen in two monkey kidney cell lines (Frhk-4 and Vero cells). DRB, a known inhibitor of the synthesis of messenger RNA, caused moderate changes in cell morphology. However, Frhk-4 cells could be maintained for several weeks at 80 microM of DRB, the concentration that caused maximal enhancement on HAV. DRB should be present from about the time of virus inoculation and its strongest effect was seen at low multiplicities of infection. Using radioimmunofocus assay it could be shown that DRB increased the amount of infectious virus. DRB treatment was applied in primary isolation of HAV from feces. In nine of ten strains HAV antigen expression was strongly increased and in six of the ten strains infectivity of harvested material increased by one 10log or more. DRB thus seems to be a useful enhancer of HAV growth in tissue culture.}}, author = {{Widell, Anders and Hansson, Bengt-Göran and Nordenfelt, Erik and Öberg, Bo}}, issn = {{1096-9071}}, keywords = {{virus enhancement; hepatitis A virus; HAV in tissue culture}}, language = {{eng}}, number = {{4}}, pages = {{369--376}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Medical Virology}}, title = {{Enhancement of hepatitis A propagation in tissue culture with 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole}}, url = {{http://dx.doi.org/10.1002/jmv.1890240403}}, doi = {{10.1002/jmv.1890240403}}, volume = {{24}}, year = {{1988}}, }