Polymerase chain reaction for detection of Mycobacterium tuberculosis
(1990) In Journal of Clinical Microbiology 28(10). p.2200-2204- Abstract
- A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1105184
- author
- Sjöbring, Ulf LU ; Mecklenburg, Michael ; Bengård Andersen, Åse and Miörner, Håkan LU
- organization
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Clinical Microbiology
- volume
- 28
- issue
- 10
- pages
- 2200 - 2204
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:2121782
- scopus:0025002931
- ISSN
- 1098-660X
- language
- English
- LU publication?
- yes
- id
- c7fa6385-7680-4655-ab44-9dd2d3400f18 (old id 1105184)
- alternative location
- http://jcm.asm.org/cgi/reprint/28/10/2200
- http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=268147&blobtype=pdf
- date added to LUP
- 2016-04-01 16:21:43
- date last changed
- 2021-09-26 03:28:58
@article{c7fa6385-7680-4655-ab44-9dd2d3400f18, abstract = {{A polymerase chain reaction for the specific detection of mycobacteria belonging to the Mycobacterium tuberculosis complex was developed. Using a single primer pair derived from the nucleotide sequence of protein antigen b of M. tuberculosis, we achieved specific amplification of a 419-base-pair DNA fragment in M. tuberculosis and M. bovis. After DNA was extracted from mycobacteria by using a simple, safe lysis procedure, we detected the 419-base-pair sequence in samples containing few mycobacteria. Preliminary data suggested that this technique could be applied to clinical specimens for early and specific diagnosis of tuberculosis.}}, author = {{Sjöbring, Ulf and Mecklenburg, Michael and Bengård Andersen, Åse and Miörner, Håkan}}, issn = {{1098-660X}}, language = {{eng}}, number = {{10}}, pages = {{2200--2204}}, publisher = {{American Society for Microbiology}}, series = {{Journal of Clinical Microbiology}}, title = {{Polymerase chain reaction for detection of Mycobacterium tuberculosis}}, url = {{http://jcm.asm.org/cgi/reprint/28/10/2200}}, volume = {{28}}, year = {{1990}}, }