A fibroblast heparan sulphate proteoglycan with a 70 kDa core protein is linked to membrane phosphatidylinositol
(1990) In Glycoconjugate Journal 7(6). p.563-572- Abstract
- Here we present evidence that a fibroblast heparan sulphate proteoglycan of approx. 300 kDa and with a core protein of apparent molecular mass 70 kDa is covalently linked to the plasma membrane via a linkage structure involving phosphatidylinositol. Phosphatidylinositol-specific phospholipase C releases such a heparan sulphate proteoglycan only from cells labelled with [35S]sulphate in the absence of serum. Cell cultures labelled with [3H]myo-inositol in the absence or presence of serum produce a radiolabelled heparan sulphate proteoglycan which was purified by gel-permeation chromatography and ion-exchange chromatography on MonoQ. Digestion with heparan sulphate lyase and analysis by gel-permeation chromatography and sodium... (More)
- Here we present evidence that a fibroblast heparan sulphate proteoglycan of approx. 300 kDa and with a core protein of apparent molecular mass 70 kDa is covalently linked to the plasma membrane via a linkage structure involving phosphatidylinositol. Phosphatidylinositol-specific phospholipase C releases such a heparan sulphate proteoglycan only from cells labelled with [35S]sulphate in the absence of serum. Cell cultures labelled with [3H]myo-inositol in the absence or presence of serum produce a radiolabelled heparan sulphate proteoglycan which was purified by gel-permeation chromatography and ion-exchange chromatography on MonoQ. Digestion with heparan sulphate lyase and analysis by gel-permeation chromatography and sodium dodecylsulphate-polyacrylamide gel-electrophoresis revealed that the 3H-label is associated with a core protein of apparent mass 70 kDa. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1105413
- author
- Schmidtchen, Artur LU ; Sundler, Roger LU and Fransson, Lars-Åke LU
- organization
- publishing date
- 1990
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- heparan sulphate, phosphatidylinositol, fibroblasts, proteoglycan
- in
- Glycoconjugate Journal
- volume
- 7
- issue
- 6
- pages
- 563 - 572
- publisher
- Springer
- external identifiers
-
- pmid:2136353
- scopus:0025696364
- ISSN
- 1573-4986
- DOI
- 10.1007/BF01189077
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Glycobiology (013212006), Macrophage Signalling (013212039), Department of Dermatology and Venereology (Lund) (013006000)
- id
- 0b089702-86ad-49d5-9788-673c26d50649 (old id 1105413)
- date added to LUP
- 2016-04-01 17:07:20
- date last changed
- 2021-01-03 05:15:00
@article{0b089702-86ad-49d5-9788-673c26d50649, abstract = {{Here we present evidence that a fibroblast heparan sulphate proteoglycan of approx. 300 kDa and with a core protein of apparent molecular mass 70 kDa is covalently linked to the plasma membrane via a linkage structure involving phosphatidylinositol. Phosphatidylinositol-specific phospholipase C releases such a heparan sulphate proteoglycan only from cells labelled with [35S]sulphate in the absence of serum. Cell cultures labelled with [3H]myo-inositol in the absence or presence of serum produce a radiolabelled heparan sulphate proteoglycan which was purified by gel-permeation chromatography and ion-exchange chromatography on MonoQ. Digestion with heparan sulphate lyase and analysis by gel-permeation chromatography and sodium dodecylsulphate-polyacrylamide gel-electrophoresis revealed that the 3H-label is associated with a core protein of apparent mass 70 kDa.}}, author = {{Schmidtchen, Artur and Sundler, Roger and Fransson, Lars-Åke}}, issn = {{1573-4986}}, keywords = {{heparan sulphate; phosphatidylinositol; fibroblasts; proteoglycan}}, language = {{eng}}, number = {{6}}, pages = {{563--572}}, publisher = {{Springer}}, series = {{Glycoconjugate Journal}}, title = {{A fibroblast heparan sulphate proteoglycan with a 70 kDa core protein is linked to membrane phosphatidylinositol}}, url = {{http://dx.doi.org/10.1007/BF01189077}}, doi = {{10.1007/BF01189077}}, volume = {{7}}, year = {{1990}}, }