Involvement of CCL25 (TECK) in the generation of the murine small-intestinal CD8alpha alpha+CD3+ intraepithelial lymphocyte compartment.
(2002) In European Journal of Immunology 32(12). p.3488-3497- Abstract
- The CC chemokine CCL25 (TECK) is selectively expressed in the thymus and small intestine, indicating a potential role in T lymphocyte development. In the present study we examined the role of CCL25 in the generation of the small-intestinal CD8alpha alpha(+)CD3(+) intraepithelial lymphocyte (IEL) compartment. CCL25 mRNA expression in the murine small intestine increased at three weeks of age and corresponded with the appearance of CD8alpha alpha(+)CD3(+) lymphocytes in the small-intestinal epithelium. Administration of monoclonal neutralizing anti-CCL25 antibody to two-week-old mice led to a approximately 50% reduction in the total number of CD8alpha alpha(+)TCRgamma delta(+) and CD8alpha alpha(+)TCRalpha beta(+) IEL at four weeks of age.... (More)
- The CC chemokine CCL25 (TECK) is selectively expressed in the thymus and small intestine, indicating a potential role in T lymphocyte development. In the present study we examined the role of CCL25 in the generation of the small-intestinal CD8alpha alpha(+)CD3(+) intraepithelial lymphocyte (IEL) compartment. CCL25 mRNA expression in the murine small intestine increased at three weeks of age and corresponded with the appearance of CD8alpha alpha(+)CD3(+) lymphocytes in the small-intestinal epithelium. Administration of monoclonal neutralizing anti-CCL25 antibody to two-week-old mice led to a approximately 50% reduction in the total number of CD8alpha alpha(+)TCRgamma delta(+) and CD8alpha alpha(+)TCRalpha beta(+) IEL at four weeks of age. Freshly isolated murine CD8alpha alpha(+)CD3(+) IEL migrated in response to CCL25 and expressed the CCL25 receptor, CCR9. Analysis of CCR9 expression on putative IEL precursor populations demonstrated the presence of both CCR9(-) and CCR9(+) cells and indicated that up-regulation of this receptor occurred during IEL precursor differentiation. Finally, data from wild-type and RAG(-/-) mice suggested that the reduction in CD8alpha alpha(+)CD3(+) IEL in anti-CCL25 antibody treated mice resulted primarily from defective maintenance and/or development of IEL precursors rather than a direct effect on mature CD8alpha alpha(+)CD3(+) IEL. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/111089
- author
- Marsal, Jan LU ; Svensson Frej, Marcus LU ; Ericsson, Anna LU ; Feridani, Amir LU ; Carramolino, Laura ; Márquez, Gabriel and Agace, William LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Chemokine, Mucosa, Intraepithelial lymphocyte
- in
- European Journal of Immunology
- volume
- 32
- issue
- 12
- pages
- 3488 - 3497
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000179907000017
- pmid:12442331
- scopus:0036910773
- ISSN
- 1521-4141
- DOI
- 10.1002/1521-4141(200212)32:12<3488::AID-IMMU3488>3.0.CO;2-E
- language
- English
- LU publication?
- yes
- id
- b1e5dcfb-5023-4b3d-ab4f-5d16fecd72a9 (old id 111089)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12442331&dopt=Abstract
- date added to LUP
- 2016-04-01 12:26:50
- date last changed
- 2022-04-13 19:06:21
@article{b1e5dcfb-5023-4b3d-ab4f-5d16fecd72a9,
abstract = {{The CC chemokine CCL25 (TECK) is selectively expressed in the thymus and small intestine, indicating a potential role in T lymphocyte development. In the present study we examined the role of CCL25 in the generation of the small-intestinal CD8alpha alpha(+)CD3(+) intraepithelial lymphocyte (IEL) compartment. CCL25 mRNA expression in the murine small intestine increased at three weeks of age and corresponded with the appearance of CD8alpha alpha(+)CD3(+) lymphocytes in the small-intestinal epithelium. Administration of monoclonal neutralizing anti-CCL25 antibody to two-week-old mice led to a approximately 50% reduction in the total number of CD8alpha alpha(+)TCRgamma delta(+) and CD8alpha alpha(+)TCRalpha beta(+) IEL at four weeks of age. Freshly isolated murine CD8alpha alpha(+)CD3(+) IEL migrated in response to CCL25 and expressed the CCL25 receptor, CCR9. Analysis of CCR9 expression on putative IEL precursor populations demonstrated the presence of both CCR9(-) and CCR9(+) cells and indicated that up-regulation of this receptor occurred during IEL precursor differentiation. Finally, data from wild-type and RAG(-/-) mice suggested that the reduction in CD8alpha alpha(+)CD3(+) IEL in anti-CCL25 antibody treated mice resulted primarily from defective maintenance and/or development of IEL precursors rather than a direct effect on mature CD8alpha alpha(+)CD3(+) IEL.}},
author = {{Marsal, Jan and Svensson Frej, Marcus and Ericsson, Anna and Feridani, Amir and Carramolino, Laura and Márquez, Gabriel and Agace, William}},
issn = {{1521-4141}},
keywords = {{Chemokine; Mucosa; Intraepithelial lymphocyte}},
language = {{eng}},
number = {{12}},
pages = {{3488--3497}},
publisher = {{John Wiley & Sons Inc.}},
series = {{European Journal of Immunology}},
title = {{Involvement of CCL25 (TECK) in the generation of the murine small-intestinal CD8alpha alpha+CD3+ intraepithelial lymphocyte compartment.}},
url = {{http://dx.doi.org/10.1002/1521-4141(200212)32:12<3488::AID-IMMU3488>3.0.CO;2-E}},
doi = {{10.1002/1521-4141(200212)32:12<3488::AID-IMMU3488>3.0.CO;2-E}},
volume = {{32}},
year = {{2002}},
}