Antibody responses of rats after immunization with organic acid anhydrides as a model of predictive testing
(1998) In Scandinavian Journal of Work, Environment and Health 24(3). p.220-227- Abstract
- OBJECTIVES: The sensitizing properties of organic acid anhydrides (OAA) were evaluated in a rat model. METHODS: The development of specific immunoglobulin (Ig)E and Ig G in serum was investigated after immunization with 14 OAA and 3 OAA conjugates. Brown Norway rats were injected intradermally with 0.1 ml of 0.2 M OAA in liquid paraffin or 1.4 mg of rat serum albumin conjugate in saline. Serum samples were collected after 4 weeks. Antibodies were analyzed with enzyme-linked immunosorbent assay. RESULTS: The serum titers of specific Ig E after immunization with the different free OAA varied from <50 to 6400. The rats immunized with 4-methylphthalic anhydride exhibited the highest titers. The specificity of Ig E was demonstrated by... (More)
- OBJECTIVES: The sensitizing properties of organic acid anhydrides (OAA) were evaluated in a rat model. METHODS: The development of specific immunoglobulin (Ig)E and Ig G in serum was investigated after immunization with 14 OAA and 3 OAA conjugates. Brown Norway rats were injected intradermally with 0.1 ml of 0.2 M OAA in liquid paraffin or 1.4 mg of rat serum albumin conjugate in saline. Serum samples were collected after 4 weeks. Antibodies were analyzed with enzyme-linked immunosorbent assay. RESULTS: The serum titers of specific Ig E after immunization with the different free OAA varied from <50 to 6400. The rats immunized with 4-methylphthalic anhydride exhibited the highest titers. The specificity of Ig E was demonstrated by enzyme-linked immunosorbent assay inhibition tests. A good correlation was observed between the Ig E and Ig G titers. Immunization with OAA conjugates showed results parallel to the findings for the free compounds. Importantly, the Ig E titers for the OAA agreed well with findings from guinea pigs and with literature data from epidemiologic studies of exposed workers. CONCLUSIONS: The present animal model may be a valuable tool for predicting the sensitizing potential of OAA and possibly the sensitizing potential of low-molecular-weight compounds in general. Furthermore, the antibody specificity of the haptens and the variations in the magnitude of the antibody titers indicate a valuable approach for studies of quantitative structure-activity relationships. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1112937
- author
- Zhang, X D ; Welinder, Hans LU ; Jönsson, Bo A LU and Skerfving, Staffan LU
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- occupational allergy, immunoglobulin G, animal model, immunoglobulin E, structure-activity relationship
- in
- Scandinavian Journal of Work, Environment and Health
- volume
- 24
- issue
- 3
- pages
- 220 - 227
- publisher
- Finnish Institute of Occupational Health
- external identifiers
-
- pmid:9710375
- scopus:0031871016
- ISSN
- 0355-3140
- language
- English
- LU publication?
- yes
- id
- ae711adf-87eb-454b-b7de-9ac84ad4f1fb (old id 1112937)
- date added to LUP
- 2016-04-01 12:19:36
- date last changed
- 2022-01-27 02:01:51
@article{ae711adf-87eb-454b-b7de-9ac84ad4f1fb, abstract = {{OBJECTIVES: The sensitizing properties of organic acid anhydrides (OAA) were evaluated in a rat model. METHODS: The development of specific immunoglobulin (Ig)E and Ig G in serum was investigated after immunization with 14 OAA and 3 OAA conjugates. Brown Norway rats were injected intradermally with 0.1 ml of 0.2 M OAA in liquid paraffin or 1.4 mg of rat serum albumin conjugate in saline. Serum samples were collected after 4 weeks. Antibodies were analyzed with enzyme-linked immunosorbent assay. RESULTS: The serum titers of specific Ig E after immunization with the different free OAA varied from <50 to 6400. The rats immunized with 4-methylphthalic anhydride exhibited the highest titers. The specificity of Ig E was demonstrated by enzyme-linked immunosorbent assay inhibition tests. A good correlation was observed between the Ig E and Ig G titers. Immunization with OAA conjugates showed results parallel to the findings for the free compounds. Importantly, the Ig E titers for the OAA agreed well with findings from guinea pigs and with literature data from epidemiologic studies of exposed workers. CONCLUSIONS: The present animal model may be a valuable tool for predicting the sensitizing potential of OAA and possibly the sensitizing potential of low-molecular-weight compounds in general. Furthermore, the antibody specificity of the haptens and the variations in the magnitude of the antibody titers indicate a valuable approach for studies of quantitative structure-activity relationships.}}, author = {{Zhang, X D and Welinder, Hans and Jönsson, Bo A and Skerfving, Staffan}}, issn = {{0355-3140}}, keywords = {{occupational allergy; immunoglobulin G; animal model; immunoglobulin E; structure-activity relationship}}, language = {{eng}}, number = {{3}}, pages = {{220--227}}, publisher = {{Finnish Institute of Occupational Health}}, series = {{Scandinavian Journal of Work, Environment and Health}}, title = {{Antibody responses of rats after immunization with organic acid anhydrides as a model of predictive testing}}, volume = {{24}}, year = {{1998}}, }