In vivo protection of nigral dopamine neurons by lentiviral gene transfer of the novel GDNF-family member neublastin/artemin
Rosenblad, Carl ; Gronborg, M ; Hansen, C ; Blom, N ; Meyer, M ; Johansen, J ; Dago, L ; Kirik, Deniz LU ; Patel, U A and Lundberg, Cecilia LU
, et al.
(2000)
In Molecular and Cellular Neuroscience
15(2).
p.199-214
- Abstract
- The glial cell line-derived neurotrophic factor (GDNF)-family of neurotrophic factors consisted until recently of three members, GDNF, neurturin, and persephin. We describe here the cloning of a new GDNF-family member, neublastin (NBN), identical to artemin (ART), recently published (Baloh et al., 1998). Addition of NBN/ART to cultures of fetal mesencephalic dopamine (DA) neurons increased the number of surviving tyrosine hydroxylase (TH)-immunoreactive neurons by approximately 70%, similar to the maximal effect obtained with GDNF. To investigate the neuroprotective effects in vivo, lentiviral vectors carrying the cDNA for NBN/ART or GDNF were injected into the striatum and ventral midbrain. Three weeks after an intrastriatal... (More)
- The glial cell line-derived neurotrophic factor (GDNF)-family of neurotrophic factors consisted until recently of three members, GDNF, neurturin, and persephin. We describe here the cloning of a new GDNF-family member, neublastin (NBN), identical to artemin (ART), recently published (Baloh et al., 1998). Addition of NBN/ART to cultures of fetal mesencephalic dopamine (DA) neurons increased the number of surviving tyrosine hydroxylase (TH)-immunoreactive neurons by approximately 70%, similar to the maximal effect obtained with GDNF. To investigate the neuroprotective effects in vivo, lentiviral vectors carrying the cDNA for NBN/ART or GDNF were injected into the striatum and ventral midbrain. Three weeks after an intrastriatal 6-hydroxydopamine lesion only about 20% of the nigral DA neurons were left in the control group, while 80-90% of the DA neurons remained in the NBN/ART and GDNF treatment groups, and the striatal TH-immunoreactive innervation was partly spared. We conclude that NBN/ART, similarly to GDNF, is a potent neuroprotective factor for the nigrostriatal DA neurons in vivo. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1117182
- author
- Rosenblad, Carl
; Gronborg, M
; Hansen, C
; Blom, N
; Meyer, M
; Johansen, J
; Dago, L
; Kirik, Deniz
LU
; Patel, U A
and Lundberg, Cecilia
LU
, et al. (More)
- Rosenblad, Carl
; Gronborg, M
; Hansen, C
; Blom, N
; Meyer, M
; Johansen, J
; Dago, L
; Kirik, Deniz
LU
; Patel, U A
; Lundberg, Cecilia
LU
; Trono, D
; Bjorklund, A
and Johansen, T E
(Less)
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular and Cellular Neuroscience
- volume
- 15
- issue
- 2
- pages
- 199 - 214
- publisher
- Elsevier
- external identifiers
-
- pmid:10673327
- scopus:0033978389
- pmid:10673327
- ISSN
- 1044-7431
- DOI
- 10.1006/mcne.1999.0817
- language
- English
- LU publication?
- yes
- id
- 44e66185-9cdf-49de-aff5-4ee7e4b6ff73 (old id 1117182)
- date added to LUP
- 2016-04-01 11:48:23
- date last changed
- 2022-03-13 01:01:16
@article{44e66185-9cdf-49de-aff5-4ee7e4b6ff73,
abstract = {{The glial cell line-derived neurotrophic factor (GDNF)-family of neurotrophic factors consisted until recently of three members, GDNF, neurturin, and persephin. We describe here the cloning of a new GDNF-family member, neublastin (NBN), identical to artemin (ART), recently published (Baloh et al., 1998). Addition of NBN/ART to cultures of fetal mesencephalic dopamine (DA) neurons increased the number of surviving tyrosine hydroxylase (TH)-immunoreactive neurons by approximately 70%, similar to the maximal effect obtained with GDNF. To investigate the neuroprotective effects in vivo, lentiviral vectors carrying the cDNA for NBN/ART or GDNF were injected into the striatum and ventral midbrain. Three weeks after an intrastriatal 6-hydroxydopamine lesion only about 20% of the nigral DA neurons were left in the control group, while 80-90% of the DA neurons remained in the NBN/ART and GDNF treatment groups, and the striatal TH-immunoreactive innervation was partly spared. We conclude that NBN/ART, similarly to GDNF, is a potent neuroprotective factor for the nigrostriatal DA neurons in vivo.}},
author = {{Rosenblad, Carl and Gronborg, M and Hansen, C and Blom, N and Meyer, M and Johansen, J and Dago, L and Kirik, Deniz and Patel, U A and Lundberg, Cecilia and Trono, D and Bjorklund, A and Johansen, T E}},
issn = {{1044-7431}},
language = {{eng}},
number = {{2}},
pages = {{199--214}},
publisher = {{Elsevier}},
series = {{Molecular and Cellular Neuroscience}},
title = {{In vivo protection of nigral dopamine neurons by lentiviral gene transfer of the novel GDNF-family member neublastin/artemin}},
url = {{http://dx.doi.org/10.1006/mcne.1999.0817}},
doi = {{10.1006/mcne.1999.0817}},
volume = {{15}},
year = {{2000}},
}