Differential usage of IκBα and IκBβ in regulation of apoptosis versus gene expression
(2003) In Biochemical and Biophysical Research Communications 301(1). p.204-211- Abstract
- n this study we use the N-substituted benzamides declopramide (3-CPA) and N-acetyl declopramide (Na-3-CPA) to investigate the involvement of the transcription factor NF-κB in the induction of apoptosis and surface immunoglobulin κ (Igκ) expression in the mouse pre-B cell line 70Z/3. We first showed that 3-CPA-induced apoptosis at doses around 500 μM and that the 3-CPA-induced apoptosis could be suppressed by over-expression of the Bcl-2 protein. Na-3-CPA was shown to be non-apoptotic at doses up to 1–2 mM. On the other hand, Na-3-CPA inhibited LPS-induced Igκ expression while 3-CPA had no effect. Further analysis showed that while 3-CPA inhibited breakdown of IκBα, Na-3-CPA inhibited breakdown of IκBβ. In addition, we used a 70Z/3 cell... (More)
- n this study we use the N-substituted benzamides declopramide (3-CPA) and N-acetyl declopramide (Na-3-CPA) to investigate the involvement of the transcription factor NF-κB in the induction of apoptosis and surface immunoglobulin κ (Igκ) expression in the mouse pre-B cell line 70Z/3. We first showed that 3-CPA-induced apoptosis at doses around 500 μM and that the 3-CPA-induced apoptosis could be suppressed by over-expression of the Bcl-2 protein. Na-3-CPA was shown to be non-apoptotic at doses up to 1–2 mM. On the other hand, Na-3-CPA inhibited LPS-induced Igκ expression while 3-CPA had no effect. Further analysis showed that while 3-CPA inhibited breakdown of IκBα, Na-3-CPA inhibited breakdown of IκBβ. In addition, we used a 70Z/3 cell line expressing a dominant negative IκBα (70Z/3ΔNIκBα). The 70Z/3ΔNIκBα cell line was shown to be more sensitive to apoptosis and cytotoxicity induced by 3-CPA as well as by LPS, probably due to a defect in NF-κB rescue mechanism. Taken together, our data implicate distinct roles for IκBα and IκBβ in regulating various NF-κB activities. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/111940
- author
- Lindgren, Hanna ; Olsson, Anders ; Pero, Ronald LU and Leanderson, Tomas LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- NF-κB, Apoptosis, IκB, N-substituted benzamides
- in
- Biochemical and Biophysical Research Communications
- volume
- 301
- issue
- 1
- pages
- 204 - 211
- publisher
- Elsevier
- external identifiers
-
- pmid:12535663
- wos:000181052800033
- scopus:0037473754
- ISSN
- 1090-2104
- DOI
- 10.1016/S0006-291X(02)03012-7
- language
- English
- LU publication?
- yes
- id
- 6bee2753-f53b-4a11-b6b7-ba6e7490503a (old id 111940)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12535663&dopt=Abstract
- date added to LUP
- 2016-04-01 16:51:35
- date last changed
- 2022-01-28 22:39:28
@article{6bee2753-f53b-4a11-b6b7-ba6e7490503a, abstract = {{n this study we use the N-substituted benzamides declopramide (3-CPA) and N-acetyl declopramide (Na-3-CPA) to investigate the involvement of the transcription factor NF-κB in the induction of apoptosis and surface immunoglobulin κ (Igκ) expression in the mouse pre-B cell line 70Z/3. We first showed that 3-CPA-induced apoptosis at doses around 500 μM and that the 3-CPA-induced apoptosis could be suppressed by over-expression of the Bcl-2 protein. Na-3-CPA was shown to be non-apoptotic at doses up to 1–2 mM. On the other hand, Na-3-CPA inhibited LPS-induced Igκ expression while 3-CPA had no effect. Further analysis showed that while 3-CPA inhibited breakdown of IκBα, Na-3-CPA inhibited breakdown of IκBβ. In addition, we used a 70Z/3 cell line expressing a dominant negative IκBα (70Z/3ΔNIκBα). The 70Z/3ΔNIκBα cell line was shown to be more sensitive to apoptosis and cytotoxicity induced by 3-CPA as well as by LPS, probably due to a defect in NF-κB rescue mechanism. Taken together, our data implicate distinct roles for IκBα and IκBβ in regulating various NF-κB activities.}}, author = {{Lindgren, Hanna and Olsson, Anders and Pero, Ronald and Leanderson, Tomas}}, issn = {{1090-2104}}, keywords = {{NF-κB; Apoptosis; IκB; N-substituted benzamides}}, language = {{eng}}, number = {{1}}, pages = {{204--211}}, publisher = {{Elsevier}}, series = {{Biochemical and Biophysical Research Communications}}, title = {{Differential usage of IκBα and IκBβ in regulation of apoptosis versus gene expression}}, url = {{http://dx.doi.org/10.1016/S0006-291X(02)03012-7}}, doi = {{10.1016/S0006-291X(02)03012-7}}, volume = {{301}}, year = {{2003}}, }