Different roles of spermine in glucocorticoid- and Fas-induced apoptosis
(2001) In Experimental Cell Research 266(2). p.333-341- Abstract
- Two experimental systems representative of the mitochondrial and death receptor apoptotic pathways are the dexamethasone-induced programmed cell death in mouse thymocytes and the antibody-mediated cross-ligation of the Fas receptor in the human leukemic T-cell line Jurkat, respectively. In both cell systems, caspase-9, -8, and -3 were activated upon induction of apoptosis and a sub-G(1) peak appeared as a sign of ongoing DNA fragmentation. Addition of 1 mM spermine together with dexamethasone inhibited caspase activation and the appearance of the sub-G(1) peak in mouse thymocytes. In contrast, Fas-induced cell death was totally unaffected by spermine addition. Spermine addition significantly elevated the spermine concentration in both... (More)
- Two experimental systems representative of the mitochondrial and death receptor apoptotic pathways are the dexamethasone-induced programmed cell death in mouse thymocytes and the antibody-mediated cross-ligation of the Fas receptor in the human leukemic T-cell line Jurkat, respectively. In both cell systems, caspase-9, -8, and -3 were activated upon induction of apoptosis and a sub-G(1) peak appeared as a sign of ongoing DNA fragmentation. Addition of 1 mM spermine together with dexamethasone inhibited caspase activation and the appearance of the sub-G(1) peak in mouse thymocytes. In contrast, Fas-induced cell death was totally unaffected by spermine addition. Spermine addition significantly elevated the spermine concentration in both thymocytes and Jurkat cells. Thus, spermine per se did not inhibit the caspases but rather their activation. The fact that spermine inhibited caspase activation only in the thymocytes implies that spermine inhibited dexamethasone-induced apoptosis upstream of caspase-9 activation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1120285
- author
- Hegardt, Cecilia LU ; Andersson, Gunnar and Oredsson, Stina LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- glucocorticoid, apoptosis, Fas death receptor, thymocytes, Jurkat cells, sub-G1 peak, cell cycle phase distribution, polyamines, caspases, flow cytometry
- in
- Experimental Cell Research
- volume
- 266
- issue
- 2
- pages
- 333 - 341
- publisher
- Academic Press
- external identifiers
-
- pmid:11399061
- scopus:0034949763
- ISSN
- 1090-2422
- DOI
- 10.1006/excr.2001.5230
- language
- English
- LU publication?
- yes
- id
- 7e34990a-62ec-42cd-a9a1-d1119cd1e118 (old id 1120285)
- date added to LUP
- 2016-04-01 12:37:17
- date last changed
- 2022-04-13 21:28:14
@article{7e34990a-62ec-42cd-a9a1-d1119cd1e118,
abstract = {{Two experimental systems representative of the mitochondrial and death receptor apoptotic pathways are the dexamethasone-induced programmed cell death in mouse thymocytes and the antibody-mediated cross-ligation of the Fas receptor in the human leukemic T-cell line Jurkat, respectively. In both cell systems, caspase-9, -8, and -3 were activated upon induction of apoptosis and a sub-G(1) peak appeared as a sign of ongoing DNA fragmentation. Addition of 1 mM spermine together with dexamethasone inhibited caspase activation and the appearance of the sub-G(1) peak in mouse thymocytes. In contrast, Fas-induced cell death was totally unaffected by spermine addition. Spermine addition significantly elevated the spermine concentration in both thymocytes and Jurkat cells. Thus, spermine per se did not inhibit the caspases but rather their activation. The fact that spermine inhibited caspase activation only in the thymocytes implies that spermine inhibited dexamethasone-induced apoptosis upstream of caspase-9 activation.}},
author = {{Hegardt, Cecilia and Andersson, Gunnar and Oredsson, Stina}},
issn = {{1090-2422}},
keywords = {{glucocorticoid; apoptosis; Fas death receptor; thymocytes; Jurkat cells; sub-G1 peak; cell cycle phase distribution; polyamines; caspases; flow cytometry}},
language = {{eng}},
number = {{2}},
pages = {{333--341}},
publisher = {{Academic Press}},
series = {{Experimental Cell Research}},
title = {{Different roles of spermine in glucocorticoid- and Fas-induced apoptosis}},
url = {{http://dx.doi.org/10.1006/excr.2001.5230}},
doi = {{10.1006/excr.2001.5230}},
volume = {{266}},
year = {{2001}},
}