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Endocardial expression and functional characterization of endothelin-1

Saetrum Opgaard, Ole ; Adner, Mikael LU ; Peters, Theodorus H.F. ; Xu, Cang-Bao LU ; Stavenow, Lars ; Gulbenkian, Sergio ; Erlinge, David LU orcid ; Edvinsson, Lars LU and Sharma, Hari S. (2001) In Molecular and Cellular Biochemistry 224(1-2). p.151-158
Abstract
Endothelin-1 (ET-1), a 21 amino acid peptide exerts a wide range of biological activities including vasoconstriction, mitogenesis and inotropic effects on the heart. In this study, we examined whether endocardial endothelial cells express ET-1 and evaluated its functional properties. Using immunofluorescence localization method, we demonstrated cytoplasmic staining of ET-1 in the human endocardial endothelial cells from the right atrium and left ventricle. Employing reverse transcriptase polymerase chain reaction (RT-PCR) expression of ET-1 mRNA and its receptors ET(A) and ET(B) mRNAs were found in human myocardial as well as in endocardial endothelial cells. Biological activity of endocardial endothelial cells derived ET-1 was established... (More)
Endothelin-1 (ET-1), a 21 amino acid peptide exerts a wide range of biological activities including vasoconstriction, mitogenesis and inotropic effects on the heart. In this study, we examined whether endocardial endothelial cells express ET-1 and evaluated its functional properties. Using immunofluorescence localization method, we demonstrated cytoplasmic staining of ET-1 in the human endocardial endothelial cells from the right atrium and left ventricle. Employing reverse transcriptase polymerase chain reaction (RT-PCR) expression of ET-1 mRNA and its receptors ET(A) and ET(B) mRNAs were found in human myocardial as well as in endocardial endothelial cells. Biological activity of endocardial endothelial cells derived ET-1 was established as the conditioned media obtained from cultured porcine endocardial endothelial cells induced a slowly developing, strong and long-lasting contraction of circular rat aortic segments, with similar characteristics to that obtained with exogenous ET-1. Furthermore, the selective endothelin-A receptor antagonist, FR 139317, blocked the conditioned media induced contractions. Our results suggest that endocardial endothelial cells express and release biologically active ET-1 which could play a pivotal role in the regulation of myocardial contractility as well as a circulatory peptide may further act in other peripheral target organs. (Less)
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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
endothelin1, endocardial endothelial cells, immunofluorescence, in vitro pharmacology, FR139317
in
Molecular and Cellular Biochemistry
volume
224
issue
1-2
pages
151 - 158
publisher
Springer
external identifiers
  • pmid:11693192
  • scopus:0034800119
ISSN
0300-8177
DOI
10.1023/A:1011952504093
language
English
LU publication?
yes
id
2838ae92-45d8-42b0-a7b3-723d8fdf050a (old id 1121355)
date added to LUP
2016-04-01 16:51:02
date last changed
2024-01-11 16:03:07
@article{2838ae92-45d8-42b0-a7b3-723d8fdf050a,
  abstract     = {{Endothelin-1 (ET-1), a 21 amino acid peptide exerts a wide range of biological activities including vasoconstriction, mitogenesis and inotropic effects on the heart. In this study, we examined whether endocardial endothelial cells express ET-1 and evaluated its functional properties. Using immunofluorescence localization method, we demonstrated cytoplasmic staining of ET-1 in the human endocardial endothelial cells from the right atrium and left ventricle. Employing reverse transcriptase polymerase chain reaction (RT-PCR) expression of ET-1 mRNA and its receptors ET(A) and ET(B) mRNAs were found in human myocardial as well as in endocardial endothelial cells. Biological activity of endocardial endothelial cells derived ET-1 was established as the conditioned media obtained from cultured porcine endocardial endothelial cells induced a slowly developing, strong and long-lasting contraction of circular rat aortic segments, with similar characteristics to that obtained with exogenous ET-1. Furthermore, the selective endothelin-A receptor antagonist, FR 139317, blocked the conditioned media induced contractions. Our results suggest that endocardial endothelial cells express and release biologically active ET-1 which could play a pivotal role in the regulation of myocardial contractility as well as a circulatory peptide may further act in other peripheral target organs.}},
  author       = {{Saetrum Opgaard, Ole and Adner, Mikael and Peters, Theodorus H.F. and Xu, Cang-Bao and Stavenow, Lars and Gulbenkian, Sergio and Erlinge, David and Edvinsson, Lars and Sharma, Hari S.}},
  issn         = {{0300-8177}},
  keywords     = {{endothelin1; endocardial endothelial cells; immunofluorescence; in vitro pharmacology; FR139317}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{151--158}},
  publisher    = {{Springer}},
  series       = {{Molecular and Cellular Biochemistry}},
  title        = {{Endocardial expression and functional characterization of endothelin-1}},
  url          = {{http://dx.doi.org/10.1023/A:1011952504093}},
  doi          = {{10.1023/A:1011952504093}},
  volume       = {{224}},
  year         = {{2001}},
}