Spermine prevents cytochrome c release in glucocorticoid-induced apoptosis in mouse thymocytes.
(2003) In Cell Biology International 27(2). p.115-121- Abstract
- Apoptosis can be induced in primary cultures of mouse thymocytes using the glucocorticoid dexamethasone. Addition of the polyamine spermine simultaneously with dexamethasone reduces the induction of apoptosis compared to treatment with dexamethasone alone. We investigated the signal transduction pathway at the mitochondrial level in order to elucidate spermine's protective effect. Mitochondrial involvement is evident due to the loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol and activation of caspase-9 in dexamethasone-treated thymocytes. The addition of spermine inhibited the release of cytochrome c from the mitochondria into the cytosol, and also the activation of caspase-9. When the mitogen... (More)
- Apoptosis can be induced in primary cultures of mouse thymocytes using the glucocorticoid dexamethasone. Addition of the polyamine spermine simultaneously with dexamethasone reduces the induction of apoptosis compared to treatment with dexamethasone alone. We investigated the signal transduction pathway at the mitochondrial level in order to elucidate spermine's protective effect. Mitochondrial involvement is evident due to the loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol and activation of caspase-9 in dexamethasone-treated thymocytes. The addition of spermine inhibited the release of cytochrome c from the mitochondria into the cytosol, and also the activation of caspase-9. When the mitogen concanavalin A (Con A) was added to dexamethasone- plus spermine-treated thymocytes, the number of apoptotic cells in the pre-G1peak was reduced compared to thymocytes treated with only dexamethasone plus spermine. Comparing concanavalin A added to dexamethasone-treated or to dexamethasone plus spermine-treated thymocytes, showed a markedly reduced pre-G1peak in the latter. Thus, the spermine-induced inhibition of cytochrome c release confers a survival advantage on thymocytes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/112464
- author
- Hegardt, Cecilia LU ; Andersson, Gunnar and Oredsson, Stina LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cell Biology International
- volume
- 27
- issue
- 2
- pages
- 115 - 121
- publisher
- Elsevier
- external identifiers
-
- wos:000181700300004
- pmid:12662968
- scopus:0037299620
- ISSN
- 1095-8355
- DOI
- 10.1016/S1065-6995(02)00291-3
- language
- English
- LU publication?
- yes
- id
- ff4dc913-41bd-4865-8f44-d48c32e5f7b0 (old id 112464)
- alternative location
- http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12662968
- date added to LUP
- 2016-04-01 11:50:03
- date last changed
- 2022-01-26 18:57:55
@article{ff4dc913-41bd-4865-8f44-d48c32e5f7b0,
abstract = {{Apoptosis can be induced in primary cultures of mouse thymocytes using the glucocorticoid dexamethasone. Addition of the polyamine spermine simultaneously with dexamethasone reduces the induction of apoptosis compared to treatment with dexamethasone alone. We investigated the signal transduction pathway at the mitochondrial level in order to elucidate spermine's protective effect. Mitochondrial involvement is evident due to the loss of mitochondrial transmembrane potential, release of cytochrome c into the cytosol and activation of caspase-9 in dexamethasone-treated thymocytes. The addition of spermine inhibited the release of cytochrome c from the mitochondria into the cytosol, and also the activation of caspase-9. When the mitogen concanavalin A (Con A) was added to dexamethasone- plus spermine-treated thymocytes, the number of apoptotic cells in the pre-G1peak was reduced compared to thymocytes treated with only dexamethasone plus spermine. Comparing concanavalin A added to dexamethasone-treated or to dexamethasone plus spermine-treated thymocytes, showed a markedly reduced pre-G1peak in the latter. Thus, the spermine-induced inhibition of cytochrome c release confers a survival advantage on thymocytes.}},
author = {{Hegardt, Cecilia and Andersson, Gunnar and Oredsson, Stina}},
issn = {{1095-8355}},
language = {{eng}},
number = {{2}},
pages = {{115--121}},
publisher = {{Elsevier}},
series = {{Cell Biology International}},
title = {{Spermine prevents cytochrome c release in glucocorticoid-induced apoptosis in mouse thymocytes.}},
url = {{http://dx.doi.org/10.1016/S1065-6995(02)00291-3}},
doi = {{10.1016/S1065-6995(02)00291-3}},
volume = {{27}},
year = {{2003}},
}