Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula retz. fruit
(2002) In Journal of Ethnopharmacology 81(3). p.327-336- Abstract
- A 70% methanol extract of Terminalia chebula fruit, was studied for its effects on growth in several malignant cell lines including a human (MCF-7) and mouse (S115) breast cancer cell line, a human osteosarcoma cell line (HOS-1), a human prostate cancer cell line (PC-3) and a non-tumorigenic, immortalized human prostate cell line (PNT1A) using assays for proliferation ([3H]-thymidine incorporation and coulter counting), cell viability (ATP determination) and cell death (flow cytometry and Hoechst DNA staining). In all cell lines studied, the extract decreased cell viability, inhibited cell proliferation, and induced cell death in a dose dependent manner. Flow cytometry and other analyses showed that some apoptosis was induced by the... (More)
- A 70% methanol extract of Terminalia chebula fruit, was studied for its effects on growth in several malignant cell lines including a human (MCF-7) and mouse (S115) breast cancer cell line, a human osteosarcoma cell line (HOS-1), a human prostate cancer cell line (PC-3) and a non-tumorigenic, immortalized human prostate cell line (PNT1A) using assays for proliferation ([3H]-thymidine incorporation and coulter counting), cell viability (ATP determination) and cell death (flow cytometry and Hoechst DNA staining). In all cell lines studied, the extract decreased cell viability, inhibited cell proliferation, and induced cell death in a dose dependent manner. Flow cytometry and other analyses showed that some apoptosis was induced by the extract at lower concentrations, but at higher concentrations, necrosis was the major mechanism of cell death. ATP assay guided chromatographic fractionation of the extract yielded ellagic acid, 2,4-chebulyl-β-Image -glucopyranose (a new natural product), and chebulinic acid which were tested by ATP assay on HOS-1 cell line in comparison to three known antigrowth phenolics of Terminalia, gallic acid, ethyl gallate, luteolin, and tannic acid. Chebulinic acid (IC50=53.2 μM±0.16)>tannic acid (IC50=59.0 μg/ml±0.19)> and ellagic acid (IC50=78.5 μM±0.24), were the most growth inhibitory phenolics of T. chebula fruit in our study. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1124937
- author
- Saleem, A ; Michael, H ; Härkönen, Pirkko LU and Pihlaja, K
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Hydrolyzable tannins, Phenolics, Apoptosis, Cytotoxicity, Terminalia chebula Retz.
- in
- Journal of Ethnopharmacology
- volume
- 81
- issue
- 3
- pages
- 327 - 336
- publisher
- Elsevier
- external identifiers
-
- scopus:0035996866
- ISSN
- 1872-7573
- DOI
- 10.1016/S0378-8741(02)00099-5
- language
- English
- LU publication?
- no
- additional info
- Department affilation moved from v1000588 (Tumour Biology, Malmö) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:39:27.
- id
- 32b73865-ea1c-4852-a0ec-30e5accd04cf (old id 1124937)
- date added to LUP
- 2016-04-01 12:07:05
- date last changed
- 2022-04-29 00:56:08
@article{32b73865-ea1c-4852-a0ec-30e5accd04cf, abstract = {{A 70% methanol extract of Terminalia chebula fruit, was studied for its effects on growth in several malignant cell lines including a human (MCF-7) and mouse (S115) breast cancer cell line, a human osteosarcoma cell line (HOS-1), a human prostate cancer cell line (PC-3) and a non-tumorigenic, immortalized human prostate cell line (PNT1A) using assays for proliferation ([3H]-thymidine incorporation and coulter counting), cell viability (ATP determination) and cell death (flow cytometry and Hoechst DNA staining). In all cell lines studied, the extract decreased cell viability, inhibited cell proliferation, and induced cell death in a dose dependent manner. Flow cytometry and other analyses showed that some apoptosis was induced by the extract at lower concentrations, but at higher concentrations, necrosis was the major mechanism of cell death. ATP assay guided chromatographic fractionation of the extract yielded ellagic acid, 2,4-chebulyl-β-Image -glucopyranose (a new natural product), and chebulinic acid which were tested by ATP assay on HOS-1 cell line in comparison to three known antigrowth phenolics of Terminalia, gallic acid, ethyl gallate, luteolin, and tannic acid. Chebulinic acid (IC50=53.2 μM±0.16)>tannic acid (IC50=59.0 μg/ml±0.19)> and ellagic acid (IC50=78.5 μM±0.24), were the most growth inhibitory phenolics of T. chebula fruit in our study.}}, author = {{Saleem, A and Michael, H and Härkönen, Pirkko and Pihlaja, K}}, issn = {{1872-7573}}, keywords = {{Hydrolyzable tannins; Phenolics; Apoptosis; Cytotoxicity; Terminalia chebula Retz.}}, language = {{eng}}, number = {{3}}, pages = {{327--336}}, publisher = {{Elsevier}}, series = {{Journal of Ethnopharmacology}}, title = {{Inhibition of cancer cell growth by crude extract and the phenolics of Terminalia chebula retz. fruit}}, url = {{http://dx.doi.org/10.1016/S0378-8741(02)00099-5}}, doi = {{10.1016/S0378-8741(02)00099-5}}, volume = {{81}}, year = {{2002}}, }