Darmin is a novel secreted protein expressed during endoderm development in Xenopus.
(2003) In Gene Expression Patterns 3(2). p.147-152- Abstract
- Endoderm development is an area of intense interest in developmental biology, but progress has been hampered by the lack of specific markers for differentiated endodermal cells. In an unbiased secretion cloning screen of Xenopus gastrula embryos we isolated a novel gene, designated Darmin. Darmin encodes a secreted protein of 56 kDa containing a peptidase M20 domain characteristic of the glutamate carboxypeptidase group of zinc metalloproteases. We also identified homologous Darmin genes in other eukaryotes and in prokaryotes suggesting that Darmin is the founding member of a family of evolutionarily conserved proteins. Xenopus Darmin showed zygotic expression in the early endoderm and later became restricted to the midgut. By secretion... (More)
- Endoderm development is an area of intense interest in developmental biology, but progress has been hampered by the lack of specific markers for differentiated endodermal cells. In an unbiased secretion cloning screen of Xenopus gastrula embryos we isolated a novel gene, designated Darmin. Darmin encodes a secreted protein of 56 kDa containing a peptidase M20 domain characteristic of the glutamate carboxypeptidase group of zinc metalloproteases. We also identified homologous Darmin genes in other eukaryotes and in prokaryotes suggesting that Darmin is the founding member of a family of evolutionarily conserved proteins. Xenopus Darmin showed zygotic expression in the early endoderm and later became restricted to the midgut. By secretion cloning of Xenopus cleavage-stage embryos we isolated another novel protein, designated Darmin-related (Darmin-r) due to its sequence similarity with Darmin. Darmin-r was maternally expressed and showed at later stages expression in the lens and pronephric glomus. The endoderm-specific expression of Darmin makes this gene a useful marker for the study of endoderm development. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1127320
- author
- Pera, Edgar LU ; Martinez, Sarah L ; Flanagan, John J ; Brechner, Mariel ; Wessely, Oliver and De Robertis, E M
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- M20 peptidase, Darmin-related, Darmin, Endoderm, Gut, Lens, Kidney, Glutamate carboxypeptidase, Zinc metalloprotease, Endodermin, Secretion cloning, Xenopus
- in
- Gene Expression Patterns
- volume
- 3
- issue
- 2
- pages
- 147 - 152
- publisher
- Elsevier
- external identifiers
-
- scopus:0037407020
- ISSN
- 1567-133X
- DOI
- 10.1016/S1567-133X(03)00011-5
- language
- English
- LU publication?
- no
- id
- 7c35156d-03ac-4c59-81c3-5ef9a151a9e1 (old id 1127320)
- date added to LUP
- 2016-04-01 16:09:42
- date last changed
- 2022-01-28 17:44:34
@article{7c35156d-03ac-4c59-81c3-5ef9a151a9e1,
abstract = {{Endoderm development is an area of intense interest in developmental biology, but progress has been hampered by the lack of specific markers for differentiated endodermal cells. In an unbiased secretion cloning screen of Xenopus gastrula embryos we isolated a novel gene, designated Darmin. Darmin encodes a secreted protein of 56 kDa containing a peptidase M20 domain characteristic of the glutamate carboxypeptidase group of zinc metalloproteases. We also identified homologous Darmin genes in other eukaryotes and in prokaryotes suggesting that Darmin is the founding member of a family of evolutionarily conserved proteins. Xenopus Darmin showed zygotic expression in the early endoderm and later became restricted to the midgut. By secretion cloning of Xenopus cleavage-stage embryos we isolated another novel protein, designated Darmin-related (Darmin-r) due to its sequence similarity with Darmin. Darmin-r was maternally expressed and showed at later stages expression in the lens and pronephric glomus. The endoderm-specific expression of Darmin makes this gene a useful marker for the study of endoderm development.}},
author = {{Pera, Edgar and Martinez, Sarah L and Flanagan, John J and Brechner, Mariel and Wessely, Oliver and De Robertis, E M}},
issn = {{1567-133X}},
keywords = {{M20 peptidase; Darmin-related; Darmin; Endoderm; Gut; Lens; Kidney; Glutamate carboxypeptidase; Zinc metalloprotease; Endodermin; Secretion cloning; Xenopus}},
language = {{eng}},
number = {{2}},
pages = {{147--152}},
publisher = {{Elsevier}},
series = {{Gene Expression Patterns}},
title = {{Darmin is a novel secreted protein expressed during endoderm development in Xenopus.}},
url = {{http://dx.doi.org/10.1016/S1567-133X(03)00011-5}},
doi = {{10.1016/S1567-133X(03)00011-5}},
volume = {{3}},
year = {{2003}},
}