Mesenchymal stem cell content of human vertebral bone marrow
(2004) In Transplantation 78(6). p.925-929- Abstract
- Mesenchymal stem cells (MSCs) are capable of down-regulating alloimmune responses and promoting the engraftment of hematopoietic stem cells. MSCs may therefore be suitable for improving donor-specific tolerance induction in solid-organ transplantation. Cells from cadaveric vertebral bone marrow (V-BM), aspirated iliac crest-BM, and peripheral blood progenitor cells were compared. Cells were characterized by flow cytometry and colony assays. MSCs generated from V-BM were assayed for differentiation capacity and immunomodulatory function. A median 5.7 x 10(8) nucleated cells (NCs) were recovered per vertebral body. The mesenchymal progenitor, colony-forming unit-fibroblast, frequency in V-BM (11.6/10(5) NC, range: 6.0-20.0) was considerably... (More)
- Mesenchymal stem cells (MSCs) are capable of down-regulating alloimmune responses and promoting the engraftment of hematopoietic stem cells. MSCs may therefore be suitable for improving donor-specific tolerance induction in solid-organ transplantation. Cells from cadaveric vertebral bone marrow (V-BM), aspirated iliac crest-BM, and peripheral blood progenitor cells were compared. Cells were characterized by flow cytometry and colony assays. MSCs generated from V-BM were assayed for differentiation capacity and immunomodulatory function. A median 5.7 x 10(8) nucleated cells (NCs) were recovered per vertebral body. The mesenchymal progenitor, colony-forming unit-fibroblast, frequency in V-BM (11.6/10(5) NC, range: 6.0-20.0) was considerably higher than in iliac crest-BM (1.4/10(5) NC, range: 0.4-2.6) and peripheral blood progenitor cells (not detectable). MSC generated from V-BM had the typical MSC phenotype (CD105(pos)CD73(pos)CD45(neg)CD34(neg)), displayed multilineage differentiation potential, and suppressed alloreactivity in mixed lymphocyte reactions. V-BM may be an excellent source for MSC cotransplantation approaches. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1129096
- author
- Ahrens, Norbert ; Tormin, Ariane ; Paulus, Margit ; Roosterman, Daniela ; Salama, Abdulgabar ; Krenn, Veit ; Neumann, Ulf and Scheding, Stefan LU
- organization
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Transplantation
- volume
- 78
- issue
- 6
- pages
- 925 - 929
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- pmid:15385815
- scopus:4644276628
- ISSN
- 1534-6080
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Hematology/Transplantation (013022014)
- id
- 8ed14f5c-44d1-4b54-8e0c-7c752a885c84 (old id 1129096)
- date added to LUP
- 2016-04-01 15:49:05
- date last changed
- 2022-07-09 08:58:03
@article{8ed14f5c-44d1-4b54-8e0c-7c752a885c84, abstract = {{Mesenchymal stem cells (MSCs) are capable of down-regulating alloimmune responses and promoting the engraftment of hematopoietic stem cells. MSCs may therefore be suitable for improving donor-specific tolerance induction in solid-organ transplantation. Cells from cadaveric vertebral bone marrow (V-BM), aspirated iliac crest-BM, and peripheral blood progenitor cells were compared. Cells were characterized by flow cytometry and colony assays. MSCs generated from V-BM were assayed for differentiation capacity and immunomodulatory function. A median 5.7 x 10(8) nucleated cells (NCs) were recovered per vertebral body. The mesenchymal progenitor, colony-forming unit-fibroblast, frequency in V-BM (11.6/10(5) NC, range: 6.0-20.0) was considerably higher than in iliac crest-BM (1.4/10(5) NC, range: 0.4-2.6) and peripheral blood progenitor cells (not detectable). MSC generated from V-BM had the typical MSC phenotype (CD105(pos)CD73(pos)CD45(neg)CD34(neg)), displayed multilineage differentiation potential, and suppressed alloreactivity in mixed lymphocyte reactions. V-BM may be an excellent source for MSC cotransplantation approaches.}}, author = {{Ahrens, Norbert and Tormin, Ariane and Paulus, Margit and Roosterman, Daniela and Salama, Abdulgabar and Krenn, Veit and Neumann, Ulf and Scheding, Stefan}}, issn = {{1534-6080}}, language = {{eng}}, number = {{6}}, pages = {{925--929}}, publisher = {{Lippincott Williams & Wilkins}}, series = {{Transplantation}}, title = {{Mesenchymal stem cell content of human vertebral bone marrow}}, volume = {{78}}, year = {{2004}}, }