Profily endometrialni exprese mRNA u vybranych matrix metaloproteinaz a tkanoveho inhibitoru metaloproteinaz-1
(2004) In Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne 69(6). p.472-476- Abstract
- OBJECTIVE: To examine the endometrial expression pattern of messenger RNA (mRNA) for selected matrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases-1 (TIMP-1). DESIGN: Experimental study. SETTING: Department of Obstetrics and gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obsterics and Gynecology, University Hospital, Luna, Sweden. METHODS: We studied MMP-1, -3, -7, -10, -11, -12, -13, -14, -16, -26 and TIMP-1 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Based on histological examination, all specimens were classified according to an ideal 28 day menstrual cycle as early (n=8), mid (n=6) and late (n=7) proliferative... (More)
- OBJECTIVE: To examine the endometrial expression pattern of messenger RNA (mRNA) for selected matrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases-1 (TIMP-1). DESIGN: Experimental study. SETTING: Department of Obstetrics and gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obsterics and Gynecology, University Hospital, Luna, Sweden. METHODS: We studied MMP-1, -3, -7, -10, -11, -12, -13, -14, -16, -26 and TIMP-1 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Based on histological examination, all specimens were classified according to an ideal 28 day menstrual cycle as early (n=8), mid (n=6) and late (n=7) proliferative phase, early (n=4), mid (n=4) and late (n=8) secretory phase and menstrual (n=3) phase. mRNA extracted from frozen tissue samples was quantitated using real time PCR. RESULTS: Four distinct patterns of MMP mRNA expression were detected in cycling endometrium. MMP-1, -3, -10, and -12 were expressed predominantly in perimenstrual period, MMP-7 and -11 had highest levels in proliferative phase, MMP-13, -14 and -16 were expressed throughout the cycle and MMP-26 was found to be maximal in periovulatory period. Levels of TIMP-1 mRNA remained unchanged during the cycle. CONCLUSION: The specific endometrial expression profiles of MMPs during menstrual cycle point to their specific biologic roles during the cycle. MMP-26 exhibits a unique expression pattern. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1129629
- author
- Pilka, Radovan LU ; Kudela, M ; Eriksson, P and Casslén, Bertil LU
- organization
- alternative title
- Endometrial expression profiles of mRNA for selected matrix metalloproteinases and tissue inhibitor of metalloproteinases-1
- publishing date
- 2004
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne
- volume
- 69
- issue
- 6
- pages
- 472 - 476
- publisher
- Ceska Lekarska Spolecnost J. E. Purkyne
- external identifiers
-
- pmid:15633417
- scopus:9644278158
- ISSN
- 1210-7832
- language
- Czech
- LU publication?
- yes
- id
- ae807576-c7de-4554-87f1-61b73d49f9e4 (old id 1129629)
- date added to LUP
- 2016-04-01 15:50:40
- date last changed
- 2022-06-20 13:29:40
@article{ae807576-c7de-4554-87f1-61b73d49f9e4, abstract = {{OBJECTIVE: To examine the endometrial expression pattern of messenger RNA (mRNA) for selected matrix metalloproteinases (MMP) and tissue inhibitor of metalloproteinases-1 (TIMP-1). DESIGN: Experimental study. SETTING: Department of Obstetrics and gynecology of the Palacky University Medical School and University Hospital, Olomouc, Czech Republic, Department of Obsterics and Gynecology, University Hospital, Luna, Sweden. METHODS: We studied MMP-1, -3, -7, -10, -11, -12, -13, -14, -16, -26 and TIMP-1 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Based on histological examination, all specimens were classified according to an ideal 28 day menstrual cycle as early (n=8), mid (n=6) and late (n=7) proliferative phase, early (n=4), mid (n=4) and late (n=8) secretory phase and menstrual (n=3) phase. mRNA extracted from frozen tissue samples was quantitated using real time PCR. RESULTS: Four distinct patterns of MMP mRNA expression were detected in cycling endometrium. MMP-1, -3, -10, and -12 were expressed predominantly in perimenstrual period, MMP-7 and -11 had highest levels in proliferative phase, MMP-13, -14 and -16 were expressed throughout the cycle and MMP-26 was found to be maximal in periovulatory period. Levels of TIMP-1 mRNA remained unchanged during the cycle. CONCLUSION: The specific endometrial expression profiles of MMPs during menstrual cycle point to their specific biologic roles during the cycle. MMP-26 exhibits a unique expression pattern.}}, author = {{Pilka, Radovan and Kudela, M and Eriksson, P and Casslén, Bertil}}, issn = {{1210-7832}}, language = {{cze}}, number = {{6}}, pages = {{472--476}}, publisher = {{Ceska Lekarska Spolecnost J. E. Purkyne}}, series = {{Ceska gynekologie / Ceska lekarska spolecnost J. Ev. Purkyne}}, title = {{Profily endometrialni exprese mRNA u vybranych matrix metaloproteinaz a tkanoveho inhibitoru metaloproteinaz-1}}, volume = {{69}}, year = {{2004}}, }