Sodium Butyrate Enhancement of Protein Expression
(2005) 2. p.625-627- Abstract
- We studied in chimeric EGFP-Photinus luciferase HeLa cells the expression of fluorescence and luminescence in different cell culture conditions (medium with or without Serum, static or dynamic conditions, in suspension or on microcarriers) as a model to follow the productivity of a recombinant protein. By studying the effect of Butyrate known as general but unpredictable stimulating agent for protein expression, we were able to establish the optimal conditions for efficacy ( concentration, time of addition, ...)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1132291
- author
- Bassens, C ; Rigaux, P ; Hendrick, V ; Cherlet, M ; Sato, K ; Kotarsky, Knut LU and Werenne, J
- organization
- publishing date
- 2005
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- host publication
- Animal Cell Technology Meets Genomics
- volume
- 2
- pages
- 625 - 627
- publisher
- Springer
- ISBN
- 978-1-4020-2791-8
- DOI
- 10.1007/1-4020-3103-3_126
- language
- English
- LU publication?
- yes
- id
- 7ff79651-cec3-4884-9e94-cdef75379eff (old id 1132291)
- date added to LUP
- 2016-04-04 10:28:41
- date last changed
- 2018-11-21 20:58:59
@inbook{7ff79651-cec3-4884-9e94-cdef75379eff, abstract = {{We studied in chimeric EGFP-Photinus luciferase HeLa cells the expression of fluorescence and luminescence in different cell culture conditions (medium with or without Serum, static or dynamic conditions, in suspension or on microcarriers) as a model to follow the productivity of a recombinant protein. By studying the effect of Butyrate known as general but unpredictable stimulating agent for protein expression, we were able to establish the optimal conditions for efficacy ( concentration, time of addition, ...)}}, author = {{Bassens, C and Rigaux, P and Hendrick, V and Cherlet, M and Sato, K and Kotarsky, Knut and Werenne, J}}, booktitle = {{Animal Cell Technology Meets Genomics}}, isbn = {{978-1-4020-2791-8}}, language = {{eng}}, pages = {{625--627}}, publisher = {{Springer}}, title = {{Sodium Butyrate Enhancement of Protein Expression}}, url = {{http://dx.doi.org/10.1007/1-4020-3103-3_126}}, doi = {{10.1007/1-4020-3103-3_126}}, volume = {{2}}, year = {{2005}}, }