IGF-I/insulin hybrid receptors in human endothelial cells
(2005) In Molecular and Cellular Endocrinology 229(1-2). p.31-37- Abstract
- Vascular complications are common in diabetes. IGF-I receptors (IGF-IR) and insulin receptors (IR) in endothelial cells might respond to altered levels of IGF-I and insulin, resulting in altered endothelial function in diabetes. We therefore studied IGF-IR and IR gene expression, ligand binding, receptor protein, and phosphorylation in human umbilical vein endothelial cells (HUVEC). IGF-IR mRNA was more abundant than IR mRNA in freshly isolated HUVEC (IGF-IR/IR ratio 7.1 +/- 1.5) and in cultured HUVEC (ratio 3.5 +/- 0.51). Accordingly, specific binding of (125)I-IGF-I (0.64 +/- 0.25%) was higher than that of (125)I-insulin (0.25 +/- 0.09%). Protein was detected for both receptors and IGF-I/insulin hybrid receptors. IGF-IR phosphorylation... (More)
- Vascular complications are common in diabetes. IGF-I receptors (IGF-IR) and insulin receptors (IR) in endothelial cells might respond to altered levels of IGF-I and insulin, resulting in altered endothelial function in diabetes. We therefore studied IGF-IR and IR gene expression, ligand binding, receptor protein, and phosphorylation in human umbilical vein endothelial cells (HUVEC). IGF-IR mRNA was more abundant than IR mRNA in freshly isolated HUVEC (IGF-IR/IR ratio 7.1 +/- 1.5) and in cultured HUVEC (ratio 3.5 +/- 0.51). Accordingly, specific binding of (125)I-IGF-I (0.64 +/- 0.25%) was higher than that of (125)I-insulin (0.25 +/- 0.09%). Protein was detected for both receptors and IGF-I/insulin hybrid receptors. IGF-IR phosphorylation was stimulated by 10(-10) to 10(-8) M IGF-I. IR were activated by 10(-9) to 10(-8) M insulin and IGF-I. We conclude that HUVEC express more IGF-IR than IR, and also express hybrid receptors. Both IGF-I and insulin phosphorylate their own receptors but only IGF-I seems to phosphorylate hybrid receptors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1134306
- author
- Dekker Nitert, Marloes LU ; Chisalita, Simona I ; Olsson, Karolina ; Bornfeldt, Karin E and Arnqvist, Hans J
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Insulin/IGF-I hybrid receptor, Endothelial cells, Human, Insulin receptor, IGF-I receptor
- in
- Molecular and Cellular Endocrinology
- volume
- 229
- issue
- 1-2
- pages
- 31 - 37
- publisher
- Elsevier
- external identifiers
-
- pmid:15607526
- scopus:10944245848
- ISSN
- 1872-8057
- DOI
- 10.1016/j.mce.2004.10.003
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Molecular Metabolism (013212001)
- id
- 77bad997-cac5-42ea-af37-1c0d96b8ebad (old id 1134306)
- date added to LUP
- 2016-04-01 12:29:11
- date last changed
- 2022-04-13 19:38:56
@article{77bad997-cac5-42ea-af37-1c0d96b8ebad, abstract = {{Vascular complications are common in diabetes. IGF-I receptors (IGF-IR) and insulin receptors (IR) in endothelial cells might respond to altered levels of IGF-I and insulin, resulting in altered endothelial function in diabetes. We therefore studied IGF-IR and IR gene expression, ligand binding, receptor protein, and phosphorylation in human umbilical vein endothelial cells (HUVEC). IGF-IR mRNA was more abundant than IR mRNA in freshly isolated HUVEC (IGF-IR/IR ratio 7.1 +/- 1.5) and in cultured HUVEC (ratio 3.5 +/- 0.51). Accordingly, specific binding of (125)I-IGF-I (0.64 +/- 0.25%) was higher than that of (125)I-insulin (0.25 +/- 0.09%). Protein was detected for both receptors and IGF-I/insulin hybrid receptors. IGF-IR phosphorylation was stimulated by 10(-10) to 10(-8) M IGF-I. IR were activated by 10(-9) to 10(-8) M insulin and IGF-I. We conclude that HUVEC express more IGF-IR than IR, and also express hybrid receptors. Both IGF-I and insulin phosphorylate their own receptors but only IGF-I seems to phosphorylate hybrid receptors.}}, author = {{Dekker Nitert, Marloes and Chisalita, Simona I and Olsson, Karolina and Bornfeldt, Karin E and Arnqvist, Hans J}}, issn = {{1872-8057}}, keywords = {{Insulin/IGF-I hybrid receptor; Endothelial cells; Human; Insulin receptor; IGF-I receptor}}, language = {{eng}}, number = {{1-2}}, pages = {{31--37}}, publisher = {{Elsevier}}, series = {{Molecular and Cellular Endocrinology}}, title = {{IGF-I/insulin hybrid receptors in human endothelial cells}}, url = {{http://dx.doi.org/10.1016/j.mce.2004.10.003}}, doi = {{10.1016/j.mce.2004.10.003}}, volume = {{229}}, year = {{2005}}, }