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Rapid DNA library construction for functional genomic and metagenomic screening

Schmitz, Jonathan E ; Daniel, Anu ; Collin, Mattias LU orcid ; Schuch, Raymond and Fischetti, Vincent A (2008) In Applied and Environmental Microbiology 74(5). p.1649-1652
Abstract
A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.
Please use this url to cite or link to this publication:
author
; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
74
issue
5
pages
1649 - 1652
publisher
American Society for Microbiology
external identifiers
  • pmid:18083885
  • scopus:40549144318
ISSN
0099-2240
DOI
10.1128/AEM.01864-07
language
English
LU publication?
no
id
107b3865-d187-43ea-9878-a055552ca567 (old id 1144726)
date added to LUP
2016-04-01 11:45:47
date last changed
2022-04-05 04:39:13
@article{107b3865-d187-43ea-9878-a055552ca567,
  abstract     = {{A rapid protocol was developed for constructing plasmid libraries from small quantities of genomic/metagenomic DNA. The technique utilizes linker amplification with topoisomerase cloning and allows for inducible transcription in Escherichia coli. As proof of principle, several anti-Bacillus lysins were cloned from bacteriophage genomes and an aerolysin was cloned from a metagenomic sample.}},
  author       = {{Schmitz, Jonathan E and Daniel, Anu and Collin, Mattias and Schuch, Raymond and Fischetti, Vincent A}},
  issn         = {{0099-2240}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1649--1652}},
  publisher    = {{American Society for Microbiology}},
  series       = {{Applied and Environmental Microbiology}},
  title        = {{Rapid DNA library construction for functional genomic and metagenomic screening}},
  url          = {{http://dx.doi.org/10.1128/AEM.01864-07}},
  doi          = {{10.1128/AEM.01864-07}},
  volume       = {{74}},
  year         = {{2008}},
}