Ultrastructural characterization of dissociated embryonic ventral mesencephalic tissue treated with neuroprotectants.
(2003) In Cell Transplantation 12(3). p.235-241- Abstract
- Poor survival and differentiation of grafted dopamine neurons limits the application of clinical transplantation in Parkinson’s disease. The survival of grafted dopamine neurons is only improved by a factor of 2–3 by adding neuroprotectants during tissue preparation. We used dye exclusion cell viability and electron microscopy to investigate the effects of the caspase inhibitor ac-YVAD-cmk and the lazaroid tirilazad mesylate on ultrastructural changes in dissociated embryonic mesencephalic cells. In addition, we examined whether the neuroprotectants selectively counteracted specific signs of neurodegeneration. Cell viability decreased significantly over time in both control and treated cell suspensions, but the number of viable cells... (More)
- Poor survival and differentiation of grafted dopamine neurons limits the application of clinical transplantation in Parkinson’s disease. The survival of grafted dopamine neurons is only improved by a factor of 2–3 by adding neuroprotectants during tissue preparation. We used dye exclusion cell viability and electron microscopy to investigate the effects of the caspase inhibitor ac-YVAD-cmk and the lazaroid tirilazad mesylate on ultrastructural changes in dissociated embryonic mesencephalic cells. In addition, we examined whether the neuroprotectants selectively counteracted specific signs of neurodegeneration. Cell viability decreased significantly over time in both control and treated cell suspensions, but the number of viable cells remaining was significantly higher in tirilazad mesylate-treated cell suspensions. In control samples, the proportion of cells with an ultrastructure consistent with healthy cells decreased from 70%, immediately after dissociation, to 30% after 8 h of incubation. Similar changes were also observed in cell suspensions treated with neuroprotectants. Thus, the neuroprotectants examined did not block the development of specific morphological signs of neurodegeneration. However, when also taking into account that dead cells lysed and disappeared from each cell suspension with time, we found that the total number of remaining viable cells with healthy nuclear chromatin or intact membrane integrity was significantly higher in the tirilazad mesylate-treated group. The results indicate that tirilazad mesylate protects only a small subpopulation of embryonic mesencephalic cells from degeneration induced by mechanical trauma during tissue dissection and dissociation. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/116044
- author
- Ahn, Young Hwan LU ; Emgård-Mattson, Mia LU and Brundin, Patrik LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Embryonic mesencephalic, Ultrastructure, Lazaroid, Caspase inhibitor, Viability, Survival
- in
- Cell Transplantation
- volume
- 12
- issue
- 3
- pages
- 235 - 241
- publisher
- Cognizant Communication Corporation
- external identifiers
-
- pmid:12797378
- wos:000182958900005
- scopus:0038025359
- ISSN
- 1555-3892
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuronal Survival (013212041), Molecular Medicine and Gene Therapy (0131000135), Department of Experimental Medical Science (013210000)
- id
- 420a2db4-e09f-449f-ae1f-292399bf809f (old id 116044)
- alternative location
- http://www.ingentaconnect.com/content/cog/ct/2003/00000012/00000003/ct388
- date added to LUP
- 2016-04-01 12:28:30
- date last changed
- 2022-04-21 07:54:14
@article{420a2db4-e09f-449f-ae1f-292399bf809f,
abstract = {{Poor survival and differentiation of grafted dopamine neurons limits the application of clinical transplantation in Parkinson’s disease. The survival of grafted dopamine neurons is only improved by a factor of 2–3 by adding neuroprotectants during tissue preparation. We used dye exclusion cell viability and electron microscopy to investigate the effects of the caspase inhibitor ac-YVAD-cmk and the lazaroid tirilazad mesylate on ultrastructural changes in dissociated embryonic mesencephalic cells. In addition, we examined whether the neuroprotectants selectively counteracted specific signs of neurodegeneration. Cell viability decreased significantly over time in both control and treated cell suspensions, but the number of viable cells remaining was significantly higher in tirilazad mesylate-treated cell suspensions. In control samples, the proportion of cells with an ultrastructure consistent with healthy cells decreased from 70%, immediately after dissociation, to 30% after 8 h of incubation. Similar changes were also observed in cell suspensions treated with neuroprotectants. Thus, the neuroprotectants examined did not block the development of specific morphological signs of neurodegeneration. However, when also taking into account that dead cells lysed and disappeared from each cell suspension with time, we found that the total number of remaining viable cells with healthy nuclear chromatin or intact membrane integrity was significantly higher in the tirilazad mesylate-treated group. The results indicate that tirilazad mesylate protects only a small subpopulation of embryonic mesencephalic cells from degeneration induced by mechanical trauma during tissue dissection and dissociation.}},
author = {{Ahn, Young Hwan and Emgård-Mattson, Mia and Brundin, Patrik}},
issn = {{1555-3892}},
keywords = {{Embryonic mesencephalic; Ultrastructure; Lazaroid; Caspase inhibitor; Viability; Survival}},
language = {{eng}},
number = {{3}},
pages = {{235--241}},
publisher = {{Cognizant Communication Corporation}},
series = {{Cell Transplantation}},
title = {{Ultrastructural characterization of dissociated embryonic ventral mesencephalic tissue treated with neuroprotectants.}},
url = {{http://www.ingentaconnect.com/content/cog/ct/2003/00000012/00000003/ct388}},
volume = {{12}},
year = {{2003}},
}