Protein Release from Galactoglucomannan Hydrogels: Influence of Substitutions and Enzymatic Hydrolysis by beta-Mannanase.
(2008) In Biomacromolecules 9(8). p.2104-2110- Abstract
- O-Acetyl-galactoglucomannan (AcGGM) is the major soft-wood hemicellulose. Structurally modified AcGGM and hydrogels of AcGGM were prepared. The degree of substitution (DS) of AcGGM was modified enzymatically with alpha-galactosidase, and chemically with an acrylate derivative, 2-hydroxyethylmethacrylate (HEMA). The hydrolysis of AcGGM with beta-mannanase was shown to increase with decreasing DS. AcGGM hydrogels were prepared from chemically modified AcGGM with varying DS of HEMA. Bovine serum albumin (BSA) was encapsulated in hydrogels. A spontaneous burst release of BSA was decreased with increased DS of HEMA. The addition of beta-mannanase significantly enhanced the BSA release from hydrogels with a DS of 0.36, reaching a maximum of 95%... (More)
- O-Acetyl-galactoglucomannan (AcGGM) is the major soft-wood hemicellulose. Structurally modified AcGGM and hydrogels of AcGGM were prepared. The degree of substitution (DS) of AcGGM was modified enzymatically with alpha-galactosidase, and chemically with an acrylate derivative, 2-hydroxyethylmethacrylate (HEMA). The hydrolysis of AcGGM with beta-mannanase was shown to increase with decreasing DS. AcGGM hydrogels were prepared from chemically modified AcGGM with varying DS of HEMA. Bovine serum albumin (BSA) was encapsulated in hydrogels. A spontaneous burst release of BSA was decreased with increased DS of HEMA. The addition of beta-mannanase significantly enhanced the BSA release from hydrogels with a DS of 0.36, reaching a maximum of 95% released BSA after eight hours compared to 60% without enzyme. Thus, both the pendant group composition and the enzyme action are valuable tools in the tailoring of hydrogel release profiles of potential interest for intestine drug delivery. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1181741
- author
- Andersson, Alexandra LU ; Edlund, Ulrica ; Sjöberg, John ; Albertsson, Ann-Christine and Stålbrand, Henrik LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biomacromolecules
- volume
- 9
- issue
- 8
- pages
- 2104 - 2110
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000258400200003
- pmid:18590309
- scopus:52649167335
- ISSN
- 1526-4602
- DOI
- 10.1021/bm701399m
- language
- English
- LU publication?
- yes
- id
- a7ef8330-b365-4fdf-ba81-896cb5a04f70 (old id 1181741)
- date added to LUP
- 2016-04-01 12:27:28
- date last changed
- 2022-02-18 22:49:25
@article{a7ef8330-b365-4fdf-ba81-896cb5a04f70, abstract = {{O-Acetyl-galactoglucomannan (AcGGM) is the major soft-wood hemicellulose. Structurally modified AcGGM and hydrogels of AcGGM were prepared. The degree of substitution (DS) of AcGGM was modified enzymatically with alpha-galactosidase, and chemically with an acrylate derivative, 2-hydroxyethylmethacrylate (HEMA). The hydrolysis of AcGGM with beta-mannanase was shown to increase with decreasing DS. AcGGM hydrogels were prepared from chemically modified AcGGM with varying DS of HEMA. Bovine serum albumin (BSA) was encapsulated in hydrogels. A spontaneous burst release of BSA was decreased with increased DS of HEMA. The addition of beta-mannanase significantly enhanced the BSA release from hydrogels with a DS of 0.36, reaching a maximum of 95% released BSA after eight hours compared to 60% without enzyme. Thus, both the pendant group composition and the enzyme action are valuable tools in the tailoring of hydrogel release profiles of potential interest for intestine drug delivery.}}, author = {{Andersson, Alexandra and Edlund, Ulrica and Sjöberg, John and Albertsson, Ann-Christine and Stålbrand, Henrik}}, issn = {{1526-4602}}, language = {{eng}}, number = {{8}}, pages = {{2104--2110}}, publisher = {{The American Chemical Society (ACS)}}, series = {{Biomacromolecules}}, title = {{Protein Release from Galactoglucomannan Hydrogels: Influence of Substitutions and Enzymatic Hydrolysis by beta-Mannanase.}}, url = {{http://dx.doi.org/10.1021/bm701399m}}, doi = {{10.1021/bm701399m}}, volume = {{9}}, year = {{2008}}, }