Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

New mechanism for amino acid influx into human epidermal Langerhans cells: L-dopa/proton counter-transport system.

Falck, Bengt LU ; Bendsöe, Niels LU and Ronquist, Gunnar (2003) In Experimental Dermatology 12(5). p.602-609
Abstract
We have characterized a stereospecific transport mechanism for L-dopa into human epidermal Langerhans cells (LCs). It is different from any other amino acid transport system. It is highly concentrative, largely pH-independent, and independent of exogenous Na+, glucose and oxygen, and fuelled by a renewable intracellular energy source inhibited by iodoacetate but not by arsenate. We propose that the mechanism is a unidirectional L-dopa/proton counter-transport system. We have recently demonstrated anaerobic glycolysis in human epidermis, which substantiates the need of proton pumps for resident LCs. The findings prompt a re-evaluation of the profound changes LCs undergo when exposed to oxygen in aerobic culture. L-dopa is not metabolized by... (More)
We have characterized a stereospecific transport mechanism for L-dopa into human epidermal Langerhans cells (LCs). It is different from any other amino acid transport system. It is highly concentrative, largely pH-independent, and independent of exogenous Na+, glucose and oxygen, and fuelled by a renewable intracellular energy source inhibited by iodoacetate but not by arsenate. We propose that the mechanism is a unidirectional L-dopa/proton counter-transport system. We have recently demonstrated anaerobic glycolysis in human epidermis, which substantiates the need of proton pumps for resident LCs. The findings prompt a re-evaluation of the profound changes LCs undergo when exposed to oxygen in aerobic culture. L-dopa is not metabolized by LCs but can rapidly be dislocated to the intercellular space by certain extracellular amino acids, i.e. LCs can profit by L-dopa in a dualistic way, altogether a remarkable biological phenomenon. (Less)
Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Experimental Dermatology
volume
12
issue
5
pages
602 - 609
publisher
Wiley-Blackwell
external identifiers
  • wos:000185198900009
  • pmid:14705800
  • scopus:0142106496
ISSN
0906-6705
DOI
10.1034/j.1600-0625.2003.00019.x
language
English
LU publication?
yes
id
128d559e-8cec-4ff4-8368-2701c08cb4f6 (old id 120344)
alternative location
http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&list_uids=14705800&dopt=Abstract
date added to LUP
2016-04-01 11:32:50
date last changed
2022-04-20 18:35:22
@article{128d559e-8cec-4ff4-8368-2701c08cb4f6,
  abstract     = {{We have characterized a stereospecific transport mechanism for L-dopa into human epidermal Langerhans cells (LCs). It is different from any other amino acid transport system. It is highly concentrative, largely pH-independent, and independent of exogenous Na+, glucose and oxygen, and fuelled by a renewable intracellular energy source inhibited by iodoacetate but not by arsenate. We propose that the mechanism is a unidirectional L-dopa/proton counter-transport system. We have recently demonstrated anaerobic glycolysis in human epidermis, which substantiates the need of proton pumps for resident LCs. The findings prompt a re-evaluation of the profound changes LCs undergo when exposed to oxygen in aerobic culture. L-dopa is not metabolized by LCs but can rapidly be dislocated to the intercellular space by certain extracellular amino acids, i.e. LCs can profit by L-dopa in a dualistic way, altogether a remarkable biological phenomenon.}},
  author       = {{Falck, Bengt and Bendsöe, Niels and Ronquist, Gunnar}},
  issn         = {{0906-6705}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{602--609}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Experimental Dermatology}},
  title        = {{New mechanism for amino acid influx into human epidermal Langerhans cells: L-dopa/proton counter-transport system.}},
  url          = {{https://lup.lub.lu.se/search/files/2538616/623944.pdf}},
  doi          = {{10.1034/j.1600-0625.2003.00019.x}},
  volume       = {{12}},
  year         = {{2003}},
}