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Ancient DNA fragments inside Classical Greek amphoras reveal cargo of 2400-year-old shipwreck

Hansson, Maria LU and Foley, Brendan LU (2008) In Journal of Archaeological Science 35(5). p.1169-1176
Abstract
The origins and spread of eastern Mediterranean civilizations 4000-2000 years ago constitute defining events in human development. Interregional connections across the sea played critical roles in building increasingly sophisticated economies and societies. Research of trade and exchange among these first centers has relied upon ancient societies' archaeological artifacts. The most ubiquitous artifacts recovered from shipwreck sites are ceramic transport jars, amphoras. However, for archaeologists and historians determining the original contents of these containers has been problematic, aided only occasionally by physical evidence (e.g. olive pits, resins) found inside excavated jars. Here, we investigate whether modern DNA analyses can... (More)
The origins and spread of eastern Mediterranean civilizations 4000-2000 years ago constitute defining events in human development. Interregional connections across the sea played critical roles in building increasingly sophisticated economies and societies. Research of trade and exchange among these first centers has relied upon ancient societies' archaeological artifacts. The most ubiquitous artifacts recovered from shipwreck sites are ceramic transport jars, amphoras. However, for archaeologists and historians determining the original contents of these containers has been problematic, aided only occasionally by physical evidence (e.g. olive pits, resins) found inside excavated jars. Here, we investigate whether modern DNA analyses can reveal original contents of amphoras containing no visible physical remains. Using chloroplast DNA markers and PCR we analyzed the walls of two amphoras recovered front a 2400 year-old shipwreck off the Greek island of Chios. Our results show that short (<= 100 bp) ancient DNA fragments can be extracted front scrapings taken from amphoras' interior walls. These DNA fragments identify the amphoras' original contents. Our analyses indicate that one of the amphoras most likely contained olive oil and oregano, even though no physical traces of remains are visible inside the jar. The second amphora might have contained mastic resin; resins of various types were preservatives commonly added to ancient wine. Our analyses are the first to demonstrate that ancient DNA fragments can be extracted from the walls of amphoras recovered front underwater shipwreck sites. This opens a new field of molecular archaeology analyses, and provides a powerful tool for obtaining information about the agricultural production, contact networks, and economies of the early civilizations. (Less)
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author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
amphoras, shipwreck, ancient DNA, Greece
in
Journal of Archaeological Science
volume
35
issue
5
pages
1169 - 1176
publisher
Academic Press
external identifiers
  • wos:000255607800004
  • scopus:40749116799
ISSN
1095-9238
DOI
10.1016/j.jas.2007.08.009
project
Ancient DNA inside archaeological ceramics from Greek shipwrecks
language
English
LU publication?
yes
id
cb9264cd-e20f-444f-ad23-de8830e9f03c (old id 1204663)
date added to LUP
2016-04-01 11:53:08
date last changed
2022-02-10 22:58:26
@article{cb9264cd-e20f-444f-ad23-de8830e9f03c,
  abstract     = {{The origins and spread of eastern Mediterranean civilizations 4000-2000 years ago constitute defining events in human development. Interregional connections across the sea played critical roles in building increasingly sophisticated economies and societies. Research of trade and exchange among these first centers has relied upon ancient societies' archaeological artifacts. The most ubiquitous artifacts recovered from shipwreck sites are ceramic transport jars, amphoras. However, for archaeologists and historians determining the original contents of these containers has been problematic, aided only occasionally by physical evidence (e.g. olive pits, resins) found inside excavated jars. Here, we investigate whether modern DNA analyses can reveal original contents of amphoras containing no visible physical remains. Using chloroplast DNA markers and PCR we analyzed the walls of two amphoras recovered front a 2400 year-old shipwreck off the Greek island of Chios. Our results show that short (&lt;= 100 bp) ancient DNA fragments can be extracted front scrapings taken from amphoras' interior walls. These DNA fragments identify the amphoras' original contents. Our analyses indicate that one of the amphoras most likely contained olive oil and oregano, even though no physical traces of remains are visible inside the jar. The second amphora might have contained mastic resin; resins of various types were preservatives commonly added to ancient wine. Our analyses are the first to demonstrate that ancient DNA fragments can be extracted from the walls of amphoras recovered front underwater shipwreck sites. This opens a new field of molecular archaeology analyses, and provides a powerful tool for obtaining information about the agricultural production, contact networks, and economies of the early civilizations.}},
  author       = {{Hansson, Maria and Foley, Brendan}},
  issn         = {{1095-9238}},
  keywords     = {{amphoras; shipwreck; ancient DNA; Greece}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{1169--1176}},
  publisher    = {{Academic Press}},
  series       = {{Journal of Archaeological Science}},
  title        = {{Ancient DNA fragments inside Classical Greek amphoras reveal cargo of 2400-year-old shipwreck}},
  url          = {{http://dx.doi.org/10.1016/j.jas.2007.08.009}},
  doi          = {{10.1016/j.jas.2007.08.009}},
  volume       = {{35}},
  year         = {{2008}},
}