The kinetics of DNA-cationic vesicle complex formation
(2003) In The Journal of Physical Chemistry Part B 107(25). p.6208-6213- Abstract
- Stopped-flow fluorescence studies were performed to investigate the kinetics of formation of complexes between DNA and cationic vesicles. We followed the kinetics as a function of charge ratio and lipid vesicle composition. Binary mixtures of a saturated cationic lipid, dioctadecyldimethlammonium bromide (DODAB), and a zwitterionic lipid, dioleoylphosphatidylethanolamine (DOPE), were used to prepare small unilamellar vesicles at 25 degreesC. The binding followed first-order reactions and occurred stepwise. The results presented indicate the existence of a three-step mechanism for cationic vesicle "binding" to DNA: first DNA rapidly ads
rbs onto the oppositely charged membrane surface (milliseconds), and
thereafter, on a... (More) - Stopped-flow fluorescence studies were performed to investigate the kinetics of formation of complexes between DNA and cationic vesicles. We followed the kinetics as a function of charge ratio and lipid vesicle composition. Binary mixtures of a saturated cationic lipid, dioctadecyldimethlammonium bromide (DODAB), and a zwitterionic lipid, dioleoylphosphatidylethanolamine (DOPE), were used to prepare small unilamellar vesicles at 25 degreesC. The binding followed first-order reactions and occurred stepwise. The results presented indicate the existence of a three-step mechanism for cationic vesicle "binding" to DNA: first DNA rapidly ads
rbs onto the oppositely charged membrane surface (milliseconds), and
thereafter, on a longer time scale (seconds), the formed DNA-lipid
complex continues to aggregate and grow; a third step is also observed,
which seems to be due to rearrangement of the complex with a further
change of the DNA conformation. The time constant of each step was
determined by the relative amounts of DNA and cationic vesicles and the
composition of the lipid bilayer of the
vesicles. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/121939
- author
- Barreleiro, Paula LU and Lindman, Björn LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- The Journal of Physical Chemistry Part B
- volume
- 107
- issue
- 25
- pages
- 6208 - 6213
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000183649500027
- scopus:0038756339
- ISSN
- 1520-5207
- DOI
- 10.1021/jp0277107
- language
- English
- LU publication?
- yes
- id
- 1c80ebb7-699c-4edd-84a1-7e92c15e6268 (old id 121939)
- date added to LUP
- 2016-04-01 16:22:48
- date last changed
- 2022-03-07 05:27:35
@article{1c80ebb7-699c-4edd-84a1-7e92c15e6268, abstract = {{Stopped-flow fluorescence studies were performed to investigate the kinetics of formation of complexes between DNA and cationic vesicles. We followed the kinetics as a function of charge ratio and lipid vesicle composition. Binary mixtures of a saturated cationic lipid, dioctadecyldimethlammonium bromide (DODAB), and a zwitterionic lipid, dioleoylphosphatidylethanolamine (DOPE), were used to prepare small unilamellar vesicles at 25 degreesC. The binding followed first-order reactions and occurred stepwise. The results presented indicate the existence of a three-step mechanism for cationic vesicle "binding" to DNA: first DNA rapidly ads<br/><br> rbs onto the oppositely charged membrane surface (milliseconds), and<br/><br> thereafter, on a longer time scale (seconds), the formed DNA-lipid<br/><br> complex continues to aggregate and grow; a third step is also observed,<br/><br> which seems to be due to rearrangement of the complex with a further<br/><br> change of the DNA conformation. The time constant of each step was<br/><br> determined by the relative amounts of DNA and cationic vesicles and the<br/><br> composition of the lipid bilayer of the<br/><br> vesicles.}}, author = {{Barreleiro, Paula and Lindman, Björn}}, issn = {{1520-5207}}, language = {{eng}}, number = {{25}}, pages = {{6208--6213}}, publisher = {{The American Chemical Society (ACS)}}, series = {{The Journal of Physical Chemistry Part B}}, title = {{The kinetics of DNA-cationic vesicle complex formation}}, url = {{http://dx.doi.org/10.1021/jp0277107}}, doi = {{10.1021/jp0277107}}, volume = {{107}}, year = {{2003}}, }