Nano- and Micro-sized Molecularly Imprinted Polymer Materials for Analytical Application
(2008)- Abstract
- Specific molecular recognition materials are important in analytical applications. It has been a longstanding dream for scientific explorers to realise a versatile method to generate materials that can recognize biological and chemical substance of interests.
Toward this goal, developing synthetic polymer materials, molecularly imprinted polymers (MIPs) is one of the most promising approaches. Especially development of nano- and micro-sized MIP material is expected to open up a wide range of application opportunity.
New strategies related with preparation of nano- and micro-sized MIP and also their potential in analytical application are described in this thesis.
First, methods for preparing uniformly sized MIP... (More) - Specific molecular recognition materials are important in analytical applications. It has been a longstanding dream for scientific explorers to realise a versatile method to generate materials that can recognize biological and chemical substance of interests.
Toward this goal, developing synthetic polymer materials, molecularly imprinted polymers (MIPs) is one of the most promising approaches. Especially development of nano- and micro-sized MIP material is expected to open up a wide range of application opportunity.
New strategies related with preparation of nano- and micro-sized MIP and also their potential in analytical application are described in this thesis.
First, methods for preparing uniformly sized MIP microspheres and nanoparticles which are selective to the drug compound, propranolol and the neouropeptide, Leu-enkephalin are described, and the binding properties of the MIPs are investigated.
Second, strategies to prepare nano- and micro-sized MIP thin-film and nanofiber composite materials are described.
Finally, the obtained nano- and micro-sized MIP materials are applied in competitive binding assay, sample preparation for preconcentrating the analyte and for constructing a QCM-based chemical sensor.
The applicability in real sample analyses has been demonstrated in two examples.
Preconcentration with the MIP allowed a trace amount of propranolol (3.4 nM) in tap water to be detected by LC/MS/MS, whereas the same drug compound in urine (1 μΜ) could be detected by a rapid non-separation assay based on new scintillation MIP nanofibers. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1243769
- author
- Yoshimatsu, Keiichi LU
- supervisor
-
- Lei Ye LU
- opponent
-
- Prof. Shea J, Kenneth, Department of Chemistry, University of California, USA
- organization
- publishing date
- 2008
- type
- Thesis
- publication status
- published
- subject
- keywords
- spin-coating, solid-phase extraction, composite, electrospinning, precipitation polymerisation, Enkephalin, fluorescent labelling, propranolol, nanoparticles, scintillation proximity assay, Molecular imprinting, Molecularly imprinted polymers, nanofibers, thin-film, microspheres
- publisher
- Pure and Applied Biochemistry, Lund University
- defense location
- Lecture hall B, Kemicentrum, Getingevägen 60, Lund Faculty of Engineering
- defense date
- 2008-10-30 13:15:00
- ISBN
- 978-91-628-7606-7
- language
- English
- LU publication?
- yes
- id
- 2a5efb88-ae3e-467f-81e9-eb221101a5ef (old id 1243769)
- date added to LUP
- 2016-04-04 10:08:47
- date last changed
- 2018-11-21 20:57:02
@phdthesis{2a5efb88-ae3e-467f-81e9-eb221101a5ef,
abstract = {{Specific molecular recognition materials are important in analytical applications. It has been a longstanding dream for scientific explorers to realise a versatile method to generate materials that can recognize biological and chemical substance of interests.<br/><br>
Toward this goal, developing synthetic polymer materials, molecularly imprinted polymers (MIPs) is one of the most promising approaches. Especially development of nano- and micro-sized MIP material is expected to open up a wide range of application opportunity.<br/><br>
New strategies related with preparation of nano- and micro-sized MIP and also their potential in analytical application are described in this thesis.<br/><br>
First, methods for preparing uniformly sized MIP microspheres and nanoparticles which are selective to the drug compound, propranolol and the neouropeptide, Leu-enkephalin are described, and the binding properties of the MIPs are investigated.<br/><br>
Second, strategies to prepare nano- and micro-sized MIP thin-film and nanofiber composite materials are described.<br/><br>
Finally, the obtained nano- and micro-sized MIP materials are applied in competitive binding assay, sample preparation for preconcentrating the analyte and for constructing a QCM-based chemical sensor.<br/><br>
The applicability in real sample analyses has been demonstrated in two examples.<br/><br>
Preconcentration with the MIP allowed a trace amount of propranolol (3.4 nM) in tap water to be detected by LC/MS/MS, whereas the same drug compound in urine (1 μΜ) could be detected by a rapid non-separation assay based on new scintillation MIP nanofibers.}},
author = {{Yoshimatsu, Keiichi}},
isbn = {{978-91-628-7606-7}},
keywords = {{spin-coating; solid-phase extraction; composite; electrospinning; precipitation polymerisation; Enkephalin; fluorescent labelling; propranolol; nanoparticles; scintillation proximity assay; Molecular imprinting; Molecularly imprinted polymers; nanofibers; thin-film; microspheres}},
language = {{eng}},
publisher = {{Pure and Applied Biochemistry, Lund University}},
school = {{Lund University}},
title = {{Nano- and Micro-sized Molecularly Imprinted Polymer Materials for Analytical Application}},
year = {{2008}},
}