On the Enzymatic Activation of NADH
(2001) In Journal of Biological Chemistry 276(12). p.9316-9321- Abstract
- Atomic (1 Å) resolution x-ray structures of horse liver alcohol dehydrogenase in complex with NADH revealed the formation of an adduct in the active site between a metal-bound water and NADH. Furthermore, a pronounced distortion of the pyridine ring of NADH was observed. A series of quantum chemical calculations on the water-nicotinamide adduct showed that the puckering of the pyridine ring in the crystal structures can only be reproduced when the water is considered a hydroxide ion. These observations provide fundamental insight into the enzymatic activation of NADH for hydride transfer.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/125030
- author
- Meijers, Rob ; Morris, Richard J ; Adolph, Hans W ; Merli, Angelo ; Lamzin, Victor S and Cedergren, Eila LU
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 276
- issue
- 12
- pages
- 9316 - 9321
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- scopus:0035937753
- pmid:11134046
- ISSN
- 1083-351X
- DOI
- 10.1074/jbc.M010870200
- language
- English
- LU publication?
- yes
- id
- 69733d3b-43a8-41b9-96c2-b16ec8d829b4 (old id 125030)
- date added to LUP
- 2016-04-01 12:38:44
- date last changed
- 2022-01-27 07:55:56
@article{69733d3b-43a8-41b9-96c2-b16ec8d829b4, abstract = {{Atomic (1 Å) resolution x-ray structures of horse liver alcohol dehydrogenase in complex with NADH revealed the formation of an adduct in the active site between a metal-bound water and NADH. Furthermore, a pronounced distortion of the pyridine ring of NADH was observed. A series of quantum chemical calculations on the water-nicotinamide adduct showed that the puckering of the pyridine ring in the crystal structures can only be reproduced when the water is considered a hydroxide ion. These observations provide fundamental insight into the enzymatic activation of NADH for hydride transfer.}}, author = {{Meijers, Rob and Morris, Richard J and Adolph, Hans W and Merli, Angelo and Lamzin, Victor S and Cedergren, Eila}}, issn = {{1083-351X}}, language = {{eng}}, number = {{12}}, pages = {{9316--9321}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Biological Chemistry}}, title = {{On the Enzymatic Activation of NADH}}, url = {{http://dx.doi.org/10.1074/jbc.M010870200}}, doi = {{10.1074/jbc.M010870200}}, volume = {{276}}, year = {{2001}}, }