Affinity partitioning of biotinylated mixed liposomes: effect of charge on biotin-NeutrAvidin interaction
(2000) In Journal of Chromatography. B 743(1-2). p.389-396- Abstract
- The partitioning behaviour of biotinylated mixed liposomes in aqueous poly(ethylene glycol)/dextran two-phase systems containing NeutrAvidin-dextran suggests that the biotin-NeutrAvidin affinity interaction is charge dependent. Biotinylated phosphatidylcholine liposomes with a low negative surface charge distributed in the NeutrAvidin-containing bottom phase at neutral pH, but the introduction of additional negative charges by including phosphatidylserine or the surfactant sodium dodecylsulfate in the liposomes caused them to distribute in the poly(ethylene glycol)-rich top phase instead. By gradually lowering the pH of the affinity two-phase system below the isoelectric point (6.3) of NeutrAvidin, negatively charged... (More)
- The partitioning behaviour of biotinylated mixed liposomes in aqueous poly(ethylene glycol)/dextran two-phase systems containing NeutrAvidin-dextran suggests that the biotin-NeutrAvidin affinity interaction is charge dependent. Biotinylated phosphatidylcholine liposomes with a low negative surface charge distributed in the NeutrAvidin-containing bottom phase at neutral pH, but the introduction of additional negative charges by including phosphatidylserine or the surfactant sodium dodecylsulfate in the liposomes caused them to distribute in the poly(ethylene glycol)-rich top phase instead. By gradually lowering the pH of the affinity two-phase system below the isoelectric point (6.3) of NeutrAvidin, negatively charged phosphatidylserine/phosphatidylcholine liposomes increasingly were attracted by NeutrAvidin to the bottom phase. It is suggested that acidic amino acids present at the rim of the biotin-binding pocket of NeutrAvidin may interact electrostatically with charged residues of the closely apposed liposome surface affecting the affinity interaction. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/125076
- author
- Barinaga-Rementeria Ramírez, Irene LU ; Ekblad, Lars and Jergil, Bengt LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Phospholipids, NeutrAvidin, Biotin
- in
- Journal of Chromatography. B
- volume
- 743
- issue
- 1-2
- pages
- 389 - 396
- publisher
- Elsevier
- external identifiers
-
- scopus:0343517652
- ISSN
- 1387-2273
- DOI
- 10.1016/S0378-4347(00)00065-7
- language
- English
- LU publication?
- yes
- id
- ae60377a-8fa9-4a4d-87fa-fa4cd73ed13f (old id 125076)
- date added to LUP
- 2016-04-01 17:12:35
- date last changed
- 2022-02-13 03:31:29
@article{ae60377a-8fa9-4a4d-87fa-fa4cd73ed13f, abstract = {{The partitioning behaviour of biotinylated mixed liposomes in aqueous poly(ethylene glycol)/dextran two-phase systems containing NeutrAvidin-dextran suggests that the biotin-NeutrAvidin affinity interaction is charge dependent. Biotinylated phosphatidylcholine liposomes with a low negative surface charge distributed in the NeutrAvidin-containing bottom phase at neutral pH, but the introduction of additional negative charges by including phosphatidylserine or the surfactant sodium dodecylsulfate in the liposomes caused them to distribute in the poly(ethylene glycol)-rich top phase instead. By gradually lowering the pH of the affinity two-phase system below the isoelectric point (6.3) of NeutrAvidin, negatively charged phosphatidylserine/phosphatidylcholine liposomes increasingly were attracted by NeutrAvidin to the bottom phase. It is suggested that acidic amino acids present at the rim of the biotin-binding pocket of NeutrAvidin may interact electrostatically with charged residues of the closely apposed liposome surface affecting the affinity interaction.}}, author = {{Barinaga-Rementeria Ramírez, Irene and Ekblad, Lars and Jergil, Bengt}}, issn = {{1387-2273}}, keywords = {{Phospholipids; NeutrAvidin; Biotin}}, language = {{eng}}, number = {{1-2}}, pages = {{389--396}}, publisher = {{Elsevier}}, series = {{Journal of Chromatography. B}}, title = {{Affinity partitioning of biotinylated mixed liposomes: effect of charge on biotin-NeutrAvidin interaction}}, url = {{http://dx.doi.org/10.1016/S0378-4347(00)00065-7}}, doi = {{10.1016/S0378-4347(00)00065-7}}, volume = {{743}}, year = {{2000}}, }