NEM and filipin increase albumin transport in lung microvessels
(2001) In American Journal of Physiology - Heart and Circulatory Physiology 280(1). p.34-41- Abstract
- This study was undertaken to evaluate the role of transcytosis as a bulk transfer mechanism for the passage of albumin from blood to tissue. Isolated rat lungs were continuously weighed and perfused with an albumin-serum buffer solution under strictly controlled hemodynamic conditions, which allowed measurements of microvascular pressure and of the capillary filtration coefficient (LpS). With the use of a tissue uptake technique, it was possible to determine lung albumin clearance under isogravimetric conditions (Cliso), or at elevated filtration rates, to obtain an "apparent albumin reflection coefficient" (sigma alb). Experiments were performed during control and after reducing lung temperature from 35° to 22°C and after infusions of the... (More)
- This study was undertaken to evaluate the role of transcytosis as a bulk transfer mechanism for the passage of albumin from blood to tissue. Isolated rat lungs were continuously weighed and perfused with an albumin-serum buffer solution under strictly controlled hemodynamic conditions, which allowed measurements of microvascular pressure and of the capillary filtration coefficient (LpS). With the use of a tissue uptake technique, it was possible to determine lung albumin clearance under isogravimetric conditions (Cliso), or at elevated filtration rates, to obtain an "apparent albumin reflection coefficient" (sigma alb). Experiments were performed during control and after reducing lung temperature from 35° to 22°C and after infusions of the transcytosis inhibitors N-ethylmaleimide (NEM) or filipin. Cooling moderately increased vascular resistance and reduced LpS and Cliso largely in proportion to the induced increases in viscosity. At 35°C, NEM (0.13 mM) caused a marked increase in Lp5 and in Cl150 and also caused a reduction in sigma alb. Furthermore, Cliso increased for the highest dose of filipin tested (1.8 µg/ml). The demonstrated relative cooling insensitivity of the transfer of albumin across the endothelium in rat lungs does not support the contention of transcytosis of proteins across the endothelium. Furthermore, neither NEM nor filipin inhibited lung microvascular albumin transport, but actually increased lung endothelial permeability. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1260180
- author
- Rippe, Bengt LU and Taylor, Aubrey
- organization
- publishing date
- 2001
- type
- Contribution to journal
- publication status
- published
- subject
- in
- American Journal of Physiology - Heart and Circulatory Physiology
- volume
- 280
- issue
- 1
- pages
- 34 - 41
- publisher
- American Physiological Society
- external identifiers
-
- scopus:0035007306
- ISSN
- 1522-1539
- language
- English
- LU publication?
- yes
- id
- 70b42a0f-f68a-42be-b0a1-1f49fd276935 (old id 1260180)
- alternative location
- http://ajpheart.physiology.org/cgi/content/abstract/280/1/H34
- date added to LUP
- 2016-04-04 11:25:16
- date last changed
- 2022-01-29 21:53:12
@article{70b42a0f-f68a-42be-b0a1-1f49fd276935,
abstract = {{This study was undertaken to evaluate the role of transcytosis as a bulk transfer mechanism for the passage of albumin from blood to tissue. Isolated rat lungs were continuously weighed and perfused with an albumin-serum buffer solution under strictly controlled hemodynamic conditions, which allowed measurements of microvascular pressure and of the capillary filtration coefficient (LpS). With the use of a tissue uptake technique, it was possible to determine lung albumin clearance under isogravimetric conditions (Cliso), or at elevated filtration rates, to obtain an "apparent albumin reflection coefficient" (sigma alb). Experiments were performed during control and after reducing lung temperature from 35° to 22°C and after infusions of the transcytosis inhibitors N-ethylmaleimide (NEM) or filipin. Cooling moderately increased vascular resistance and reduced LpS and Cliso largely in proportion to the induced increases in viscosity. At 35°C, NEM (0.13 mM) caused a marked increase in Lp5 and in Cl150 and also caused a reduction in sigma alb. Furthermore, Cliso increased for the highest dose of filipin tested (1.8 µg/ml). The demonstrated relative cooling insensitivity of the transfer of albumin across the endothelium in rat lungs does not support the contention of transcytosis of proteins across the endothelium. Furthermore, neither NEM nor filipin inhibited lung microvascular albumin transport, but actually increased lung endothelial permeability.}},
author = {{Rippe, Bengt and Taylor, Aubrey}},
issn = {{1522-1539}},
language = {{eng}},
number = {{1}},
pages = {{34--41}},
publisher = {{American Physiological Society}},
series = {{American Journal of Physiology - Heart and Circulatory Physiology}},
title = {{NEM and filipin increase albumin transport in lung microvessels}},
url = {{http://ajpheart.physiology.org/cgi/content/abstract/280/1/H34}},
volume = {{280}},
year = {{2001}},
}